Study for genetic diversity of 'uzu'semi-dwarfbarley varieties in evolutionary, morphological and physiological aspects.

uzu半矮化大麦品种进化、形态和生理方面的遗传多样性研究。

• 批准号: 16380008
• 负责人: TAKEDA Kazuyoshi
• 金额: $ 9.98万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380008/
• 关键词: barley; uzu; diversity; barley; uzu; diversity

In order to dissect the diversity of semi-dwarf barley variety 'uzu' in evolutionary, morphological and physiological aspects , we analyzed the genetic diversity of barley uzu varieties from three view points:(1) phylogeny of 'uzu' accessions,(2) genetic dissection of the 'uzu' accession specific morphological trait including the emergence of projection on coleoptile under high temperature condition, and (3) the physiological diversity of tissue culture traits including callus growth and shoot regeneration capacity. Results from these studies are:(1) Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in 282 barley accessions including 19 wild barley lines reveal that landraces distributed in South and East Asia are genetically distinct from those in 'Occidental' region. The six 'uzu' accessions investigated in this study possessed almost same haplotype among the five loci. These evidence strongly indicated that the origin of 'uzu' accessions was si … More ngle mutation even.(2) One of the 'uzu'-specific morphological traits, the emergence of projection on coleoptile under high temperature germination condition, is caused by ectopic cell division of coleoptiles apart from embryo genesis. Evaluation of this trait among 266 'uzu' accessions and segregation analysis of five F2 populations indicate that genetic factor (s) concerning to this character are genetically diverse within 'uzu' accessions. As the result of diallel analysis using 10 x 10 half diallel table, it is suggested that this trait is controlled by several additive genes.(3) Effects of exogenous auxin and cytokinin on the emergence of projection on coleoptile were examined in three independent isogenic lines of barley with uzu gene. Although the emergence of projection was reduced in uzu lines under low temperature condition, both auxin and cytokinin enhanced the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection on coleoptile Less

为了阐述进化，形态和物理方面的半矮大麦品种“ uzu”的多样性，我们分析了三个观点的大麦uzu变化的遗传多样性：（1）“ UZU”的系统发育，“ UZU”附件的遗传学因素，包括“ Uzu”访问量的概念，包括特定的辅助特定培训范围，这些概念的出现了专门的培训，该概念的良好型号的出现了，这些概念的概念是专门的培训，该遗传学的遗传范围是投影范围的投入，这些概念的投入量是概述的概念。 （3）组织培养特征的身体多样性包括愈伤组织的生长和射击能力。这些研究的结果是：（1）对282个大麦入口的五个核局部和形态评估的植物地理分析，包括19种野生大麦线，表明在南亚和东亚分布在遗传上与“西部”地区的陆地差异不同。在这项研究中研究的六个“ Uzu”配件在五个基因座中假定了几乎相同的单倍型。这些证据强烈表明，“ Uzu”加入的起源是Si…甚至更多的NGLE突变。（2）“ Uzu”特异性的形态学特征之一，高温发芽条件下的鞘翅目投影的出现，是由与Embryo Genesis的生态细胞分裂引起的。对266个“ Uzu”加入和五个F2种群的隔离分析的评估表明，与此特征有关的遗传因素在“ Uzu”附属中是遗传多样的。通过使用10 x 10半拨号表进行了拨号线分析的结果，建议该性状受几个其他基因控制。（3）外源生长素和细胞分裂素对投射出现对鞘翅目的影响的影响，在三种独立的大麦源性线中，用uzu Gene在三种独立的同源线中进行了检查。尽管在低温条件下UZU线的投影出现降低，但生长素和细胞分裂素都增强了投影的出现。这些结果表明生长素和细胞分裂素调节投影的出现。这些结果表明，生长素和细胞分裂素调节了对鞘翅目投影的出现