Study for genetic diversity of 'uzu'semi-dwarfbarley varieties in evolutionary, morphological and physiological aspects.

uzu半矮化大麦品种进化、形态和生理方面的遗传多样性研究。

• 批准号: 16380008
• 负责人: TAKEDA Kazuyoshi
• 金额: $ 9.98万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380008/
• 关键词: barley; uzu; diversity

In order to dissect the diversity of semi-dwarf barley variety 'uzu' in evolutionary, morphological and physiological aspects , we analyzed the genetic diversity of barley uzu varieties from three view points:(1) phylogeny of 'uzu' accessions,(2) genetic dissection of the 'uzu' accession specific morphological trait including the emergence of projection on coleoptile under high temperature condition, and (3) the physiological diversity of tissue culture traits including callus growth and shoot regeneration capacity. Results from these studies are:(1) Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in 282 barley accessions including 19 wild barley lines reveal that landraces distributed in South and East Asia are genetically distinct from those in 'Occidental' region. The six 'uzu' accessions investigated in this study possessed almost same haplotype among the five loci. These evidence strongly indicated that the origin of 'uzu' accessions was si … More ngle mutation even.(2) One of the 'uzu'-specific morphological traits, the emergence of projection on coleoptile under high temperature germination condition, is caused by ectopic cell division of coleoptiles apart from embryo genesis. Evaluation of this trait among 266 'uzu' accessions and segregation analysis of five F2 populations indicate that genetic factor (s) concerning to this character are genetically diverse within 'uzu' accessions. As the result of diallel analysis using 10 x 10 half diallel table, it is suggested that this trait is controlled by several additive genes.(3) Effects of exogenous auxin and cytokinin on the emergence of projection on coleoptile were examined in three independent isogenic lines of barley with uzu gene. Although the emergence of projection was reduced in uzu lines under low temperature condition, both auxin and cytokinin enhanced the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection on coleoptile Less

为了剖析半矮化大麦品种‘uzu’在进化、形态和生理方面的多样性，我们从三个角度分析了大麦uzu品种的遗传多样性：(1)‘uzu’种质的系统发育，(2) “uzu”种质特定形态特征的遗传解析，包括高温条件下胚芽鞘上突起的出现，以及（3）组织培养特征的生理多样性，包括愈伤组织生长和芽再生这些研究的结果是：(1) 对 282 个大麦品种（包括 19 个野生大麦品系）的 5 个核基因座的系统发育地理学分析和两个性状的形态学评估表明，分布在南亚和东亚的地方品种在遗传上与“西方”品种不同。本研究中调查的六个“uzu”种质在五个基因座中具有几乎相同的单倍型。这些证据表明“uzu”种质的起源是…更多。 (2)'uzu'特有的形态特征之一是在高温萌发条件下胚芽鞘上出现突起，这是由胚芽鞘的异位细胞分裂引起的，而不是胚胎发生。对该性状在266'中的评价。 uzu' 种质和五个 F2 群体的分离分析表明，使用 10 x 的双列分析结果表明，与该性状相关的遗传因子在 'uzu' 种质中具有遗传多样性。从图10半双列表可以看出，该性状是由几个附加基因控制的。(3)在带有uzu基因的大麦的三个独立的同基因系中检测了外源生长素和细胞分裂素对胚芽鞘上突起的出现的影响。低温条件下uzu品系中突起的出现减少，生长素和细胞分裂素均增强了突起的出现。这些结果表明，生长素和细胞分裂素调节突起的出现。细胞分裂素调节胚芽鞘上突起的出现 Less

项目成果

Geographical differentiation and diallel analysis of seed dormancy in barley.

大麦种子休眠的地理分化和双列分析。

• 发表时间: 2006
• 期刊: Euphytica
• 影响因子: 1.9
• 作者: Taketa;S.;S. Amano;Y. Tsujino;T. Sato;D. Saisho;K. Kakeda;M. Nomura;T. Suzuki;T. Matsumoto;K. Sato;H. Kanamori;S. Kawasaki and K. Takeda.;Saisho D.;Hori K.;Takeda K.
• 通讯作者: Takeda K.

QTL analysis of Fusarium head blight resistance severity in recombinant inbread population derived from a cross between two-rowed barley varieties.

对源自两行大麦品种之间杂交的重组内面包群体中镰刀菌赤霉病抗性严重程度进行 QTL 分析。

• 发表时间: 2006
• 期刊: Breed. Sci. 56
• 作者: Taketa;S.;S. Amano;Y. Tsujino;T. Sato;D. Saisho;K. Kakeda;M. Nomura;T. Suzuki;T. Matsumoto;K. Sato;H. Kanamori;S. Kawasaki and K. Takeda.;Saisho D.;Hori K.
• 通讯作者: Hori K.

Differentiation of β-amylase phenotypes in cultivated barley.

栽培大麦中β-淀粉酶表型的分化。

• 发表时间: 2004
• 期刊: Crop Science 44
• 作者: Zhang;W.;T.Kaneko;M.Ishii;K.Takeda
• 通讯作者: K.Takeda