Development of a Novel Evaluation Technology of Cell Attachment Ability onto Polymeric Materials Based on Quartz Crystal Microbalance (QCM) Technique

基于石英晶体微天平（QCM）技术的高分子材料细胞附着能力评价新技术的开发

基本信息

批准号：
16360417
负责人：
KANAMORI Toshiyuki
金额：
$ 6.85万
依托单位：
National Institute of Advanced Industrial Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

We have already developed a novel photo-responsive cell culture surface (POPS) and been successful to control the cell attachment ability of the POPS by light irradiation. In this study, we were aiming to reveal the mechanism switching the cell attachment property using quartz crystal microbalance (QCM) technique.Based on the Sauerbrey law, the change in the frequency of a quartz sensor of QCM is assumed to be proportional to the change in the weight of the sensor, so that QCM can detect extremely little adsorption onto the sensor. As the result of this study in 2004, we have found that we can evaluate the attachment and detachment of a small number of cells on the PCPS under light irradiation using QCM. In 2005, we applied the QCM technique to not only the PCPS but also several materials and have found that cell attachment on the surface of a material is dominated by physicochemical interaction between the cells and material, which occurs just after the cells approach the surface. However, it was found that the conventional QCM technique based on the Sauerbrey law is not applicable to this system, because the PCPS and cells are well-swelled elastic bodies. Then, we paid attention to QCM-D, which can detect the change in the elasticity of a material on a quartz sensor, and have had several new and valuable information about cell-surface interaction using a demonstration machine of QCM-D briefly. Now, we are preparing a manuscript about this result for publication. Incidentally, we purchased the QCM-D machine with the other budget and are proceeding with further experiments.Based on the results of this project, it has been possible for us to design PCPS much better, and we have started a new project concerning to "Development of Two-Dimensional Cell Manipulation System" with the budget of the Creation and Support Program for Start-ups from Universities in 2005 of Japan Science and Technology Agency

我们已经开发出一种新型光响应细胞培养表面（POPS），并成功通过光照射控制POPS的细胞附着能力。在本研究中，我们旨在揭示使用石英晶体微天平（QCM）技术切换细胞附着特性的机制。根据Sauerbrey定律，假设QCM石英传感器的频率变化与变化成正比。传感器的重量，因此 QCM 可以检测到传感器上的吸附极少。作为2004年这项研究的结果，我们发现我们可以使用QCM评估光照射下PCPS上少量细胞的附着和脱离。 2005年，我们将QCM技术不仅应用于PCPS，还应用于几种材料，发现细胞在材料表面的附着主要是细胞与材料之间的物理化学相互作用，这种相互作用发生在细胞接近表面之后。然而，人们发现基于Sauerbrey定律的传统QCM技术不适用于该系统，因为PCPS和细胞是充分膨胀的弹性体。然后，我们关注了QCM-D，它可以在石英传感器上检测材料弹性的变化，并简要地使用QCM-D的演示机获得了一些关于细胞与表面相互作用的新的和有价值的信息。现在，我们正在准备一份关于这一结果的手稿以供出版。顺便说一句，我们用其他预算购买了 QCM-D 机器，并正在进行进一步的实验。根据这个项目的结果，我们可以更好地设计 PCPS，并且我们已经开始了一个关于“日本科学技术振兴机构2005年大学创业支援计划预算的“二维细胞操作系统的开发”