Development of a Novel Evaluation Technology of Cell Attachment Ability onto Polymeric Materials Based on Quartz Crystal Microbalance (QCM) Technique

基于石英晶体微天平（QCM）技术的高分子材料细胞附着能力评价新技术的开发

• 批准号: 16360417
• 负责人: KANAMORI Toshiyuki
• 金额: $ 6.85万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16360417/
• 关键词: photo-responsive cell culture surface; two-dimensional cell manipulation; quartz crystal microbalance technique; elasticity change; cell-surface interaction; physicochemical interaction; 二次元培養細胞マニピュレー; 水晶振動子微小重力測定法; 光応答性材料; ポリ(N-イソプロピルアクリルアミド); ; photo-responsive cell culture surface; two-dimensional cell manipulation; quartz crystal microbalance technique; elasticity change; cell-surface interaction; physicochemical interaction; 二次元培養細胞マニピュレー; 水晶振動子微小重力測定法; 光応答性材料; ポリ(N-イソプロピルアクリルアミド)

We have already developed a novel photo-responsive cell culture surface (POPS) and been successful to control the cell attachment ability of the POPS by light irradiation. In this study, we were aiming to reveal the mechanism switching the cell attachment property using quartz crystal microbalance (QCM) technique.Based on the Sauerbrey law, the change in the frequency of a quartz sensor of QCM is assumed to be proportional to the change in the weight of the sensor, so that QCM can detect extremely little adsorption onto the sensor. As the result of this study in 2004, we have found that we can evaluate the attachment and detachment of a small number of cells on the PCPS under light irradiation using QCM. In 2005, we applied the QCM technique to not only the PCPS but also several materials and have found that cell attachment on the surface of a material is dominated by physicochemical interaction between the cells and material, which occurs just after the cells approach the surface. However, it was found that the conventional QCM technique based on the Sauerbrey law is not applicable to this system, because the PCPS and cells are well-swelled elastic bodies. Then, we paid attention to QCM-D, which can detect the change in the elasticity of a material on a quartz sensor, and have had several new and valuable information about cell-surface interaction using a demonstration machine of QCM-D briefly. Now, we are preparing a manuscript about this result for publication. Incidentally, we purchased the QCM-D machine with the other budget and are proceeding with further experiments.Based on the results of this project, it has been possible for us to design PCPS much better, and we have started a new project concerning to "Development of Two-Dimensional Cell Manipulation System" with the budget of the Creation and Support Program for Start-ups from Universities in 2005 of Japan Science and Technology Agency

我们已经开发了一种新型的照片响应性细胞培养表面（POPS），并成功地通过光照射来控制流行的细胞附着能力。在这项研究中，我们旨在揭示使用石英晶体微量平衡（QCM）技术切换细胞附着特性的机制。基于熟伏法律，QCM的石英传感器频率的变化被认为与QCM的重量变化相比，因此可以检测到QCM的变化，从而可以检测到极少的ADSORSORSORTORS。作为2004年这项研究的结果，我们发现我们可以使用QCM在光照射下评估少数细胞在PCP上的附着和分离。在2005年，我们不仅将QCM技术应用于PCP，还将其应用于几种材料，发现材料表面的细胞附着是由细胞和材料之间的物理化学相互作用支配的，该细胞和材料之间发生在细胞接近表面之后。然而，发现基于酸菜定律的常规QCM技术不适用于该系统，因为PCP和细胞是良好的弹性体。然后，我们注意QCM-D，该QCM-D可以检测石英传感器上材料的弹性的变化，并使用QCM-D的演示机简要介绍了几个有关细胞表面相互作用的新的且有价值的信息。现在，我们正在准备有关此结果的手稿。顺便说一句，我们购买了带有其他预算的QCM-D机器，并正在进行进一步的实验。基于该项目的结果，我们有可能更好地设计PCP，我们已经开始了一个新的项目，以“开发二维细胞操作系统”，并通过2005年日本科学和技术机构的创建计划的预算和支持计划开发了日本科学和技术机构的预算计划。