Development of Low-temperature Sterilization Technique Using Microwave Plasma and Its Medical Application

微波等离子体低温灭菌技术的发展及其医学应用

• 批准号: 16340180
• 负责人: NAGATSU Masaaki
• 金额: $ 9.79万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16340180/
• 关键词: Plasma sterilization; Surface wave plasma; volume wave plasma; Oxygen radicals; Microwave plasma; Low temperature sterilization; Ultra violet emission; Medical application; プラズマ減菌; パルス変調

In this research, we aimed at developing a novel low-temperature sterilization technique using large-volume microwave plasma and finally applying the results to the medical field. In the fiscal year 2004, we have carried out the experiments to clarify the mechanism of plasma sterilization and to study the possibility of improving the characteristics of rapid sterilization at low temperature. In the fiscal years 2005 and 2006, we have performed the experimental verification of low-temperature internal sterilization for the wrapped medical instruments using the volume-wave plasma in order to confirm the sterilization of specific medical equipments such as catheter. In the following, we listed the main results of the present research.(1) Clarification of the sterilization mechanism of oxygen plasma: It was experimentally confirmed that the etching effect due to the oxygen radicals was the main sterilizing factor in the oxygen plasma. While no strong UV emission was observed in the oxygen … More plasma, it was shown that the UV emissions were main factor in the nitrogen plasma, since there were several strong UV emissions in the range of 290〜400 nm.(2) Reduction of thermal effect by means of on-off control of microwave plasma: It is required to avoid thermal effect in the case of plastic medical instruments, because of non-heat resist materials. By introducing the time-modulated operation of microwave plasma, we succeeded to reduce the temperature of the samples less than 70 ℃.(3) Experiment of volume-wave plasma production: To sterilize inside the wrapped articles, we have carried out the sterilization experiment using the volume-wave plasma. We confirmed that the plasma was produced inside the plastic bottle. By using the pulsed-microwave plasma, we confirmed the sterilization of biological indicators (Bacillus Atrophaous) wrapped by glassine paper within 20 min.(4) Effect of OH radicals: We have investigated the effect of water vapor addition on the sterilization performance using the microwave plasma with air-simulated nitrogen-oxygen gas mixture. From the results, it was deduced that the water vapor played an important role on the improvement of sterilization speed. The collapse of spore cells might be attributed to the OH radicals generated in the plasma.(5) Summary of results: We reported 16 journal papers, 2 books, 38 proceeding of international conferences, and 132 presentations in the domestic meetings. We also applied 7 domestic and foreign patents relating with the present plasma sterilization, and we have taken one patent on the plasma sterilization method. Less

在这项研究中，我们的目标是开发一种利用大容量微波等离子体的新型低温灭菌技术，并最终将其成果应用于医疗领域。在2004财年，我们进行了实验以阐明等离子体灭菌的机理。并研究提高低温快速灭菌特性的可能性。2005年和2006财年，我们对低温内部灭菌进行了实验验证。使用体积波等离子体包裹医疗器械，以确认导管等特定医疗器械的灭菌效果。下面列出了本研究的主要结果。（1）阐明了氧等离子体的灭菌机理。实验证实，氧自由基的蚀刻效应是氧等离子体的主要杀菌因素，而在氧等离子体中没有观察到强烈的紫外线发射，而在氮等离子体中则表明紫外线发射是主要因素。 ，因为有290~400 nm范围内有数种强紫外发射。 (2)通过微波等离子体的开关控制来减少热效应：对于塑料医疗器械，由于非热效应，要求避免热效应。通过引入微波等离子体的时间调制操作，我们成功地将样品的温度降低到70℃以下。（3）体积波等离子体产生实验：为了对包装物品内部进行灭菌，我们进行了实验。出使用体积波等离子体进行灭菌实验，我们确认了在塑料瓶内部产生了等离子体。通过使用脉冲微波等离子体，我们证实了在 20 分钟内对玻璃纸包裹的生物指示剂（萎缩芽孢杆菌）进行了灭菌。(4) ) OH 自由基的影响：我们使用微波等离子体和模拟空气的氮氧气体混合物研究了水蒸气的添加对灭菌性能的影响，从结果中推断出水蒸气的存在。蒸气对灭菌速度的提高起到了重要作用。孢子细胞的崩溃可能归因于等离子体中产生的OH自由基。(5)结果摘要：我们报道了16篇期刊论文、2本书籍、38篇国际会议论文集。 ，国内会议报告132项，目前等离子体灭菌相关国内外专利7项，其中等离子体灭菌方法专利1项。

项目成果

Gas barrier properties of hydrogenated amorphous carbon films coated on polymers by surface-wave plasma CVD

表面波等离子体CVD聚合物表面氢化非晶碳薄膜的阻气性能

• 发表时间: 2007
• 期刊: Thin Solid Films 515
• 作者: H.Zhou;A.Ogino
• 通讯作者: A.Ogino

Novel Plasma Sterilization Technique Using Volume Wave Plasma Excited by 2.45 GHz Microwaves

使用 2.45 GHz 微波激发体波等离子体的新型等离子体灭菌技术

• 发表时间: 2005
• 期刊: Abstracts and Full Papers CD of 17th Int'l.Symp. on Plasma Chemistry
• 作者: L.Xu;P.Liu;R.J.Zhan;X.H.Wen;L.L.Ding;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu
• 通讯作者: M.Nagatsu

マイクロ波プラズマの滅菌応用

微波等离子体灭菌应用

• 发表时间: 2005
• 期刊: 化学工業 56・6
• 作者: M.Nagatsu;T.Yoshida;M.Mesko;A.Ogino;T.Matsuda;T.Tanaka;H.Tatsuoka;K.Murakami;L.Xu;M.Siry;M.Siry;H.Nakano;T.Nagata;M.Nagatsu;L.Xu;M.Nagatsu;L.Xu;M.Nagatsu;H.Nonaka;A.Ogino;L.Xu;M.Siry;M.Nagatsu;M.Nagatsu;永津 雅章
• 通讯作者: 永津 雅章

Internal Sterilization Using Microwave-Excited Volume-Wave Plasma

使用微波激发体积波等离子体进行内部灭菌

• 发表时间: 2004
• 期刊: Proc.of International Symposium on Microwave Science and Its Application to Related Fields
• 作者: L.Xu;P.Liu;R.J.Zhan;X.H.Wen;L.L.Ding;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;L.Xu;M.Nagatsu;A.Ogino;M.Nagatsu;A.Ogino;L.Xu;H.Nonaka;M.Shido;T.Aoshi;M.Nagatsu;L.Xu;M.Nagatsu;L.Xu;A.Ogino;M.Nagatsu;M.Nagatsu;L.Xu;F.Terashita;H.Nonaka;M.Shido;M.Stefecka;M.Nagatsu;M.Nagatsu;L.Xu;F.Terashita
• 通讯作者: F.Terashita

Immunization with gene encoding granulocyte-macrophage colony- stimulating factor inserted with a single helper T-cell epitope of an intracellular bacterium induces a specific T-cell subset and protective immunity

使用编码粒细胞-巨噬细胞集落刺激因子的基因进行免疫，并插入细胞内细菌的单个辅助 T 细胞表位，可诱导特定的 T 细胞亚群和保护性免疫

• 发表时间: 2006
• 期刊: Vaccine 24
• 作者: M.Nagatsu;T.Yoshida;M.Mesko;A.Ogino;T.Matsuda;T.Tanaka;H.Tatsuoka;K.Murakami;L.Xu;M.Siry;M.Siry;H.Nakano;T.Nagata
• 通讯作者: T.Nagata