A STUDY OF IDENTIFICATION AND QUANTIFICATION OF MIDDLE REPETITIVE SEQUENCES OF DROSOPHILA GENOME

果蝇基因组中间重复序列的鉴定和定量研究

• 批准号: 03640540
• 负责人: HARADA Ko
• 金额: $ 1.22万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640540/
• 关键词: middle repetitive sequence; Drosophila melanogaster; transposable elements; random clone; in situ hybridilzation; genome structure; in situ hybridization; ランダムクロ-ン; オナジショウジョウバエ

In order to study the genome structure concerning to the repetitive sequences we mapped random clones extracted from the genomic library of Drosophila melanogaster on the salivary gland chromosome. About 800 clones were randomly chosen from the phage library and chromosomal locations were determined for 231 clones by in situ hybridization. 142 clones were mapped on the unique locations and 89 clones were mapped on the multiple chromosomeal locations. Two groups of clones were distinguished from the distribution of these clones containing sequences which have multiple copies on the chromosome. One group is made of clones which has relatively small copy numbers (2-6) and the others have more than 10 copies on the chromosome. Mapping these clones to the chromosome of Drosopila simulans, the closely related species of D.melanogaster, it was found that the former group of clones were mapped on the same location and/or have the same copy numbers. On the other hand, the latter had generally smaller copy numbers and were mapped on different locations. These results suggest that the former clones are made of mainly multigene families and the latters contains transposons. We are now analyzing detailed molecular structure of these clones.

为了研究与重复序列有关的基因组结构，我们将从果蝇基因组文库中提取的随机克隆映射到唾液腺染色体上。从噬菌体文库中随机选择约800个克隆，并通过原位杂交确定了231个克隆的染色体位置。 142 个克隆被定位在独特位置上，89 个克隆被定位在多个染色体位置上。根据这些克隆的分布来区分两组克隆，这些克隆含有在染色体上具有多个拷贝的序列。一组由具有相对较小拷贝数（2-6）的克隆组成，而其他组则在染色体上具有超过10个拷贝。将这些克隆映射到与黑腹果蝇密切相关的拟果蝇（Drosopila simulans）的染色体上，发现前一组克隆被映射到相同的位置和/或具有相同的拷贝数。另一方面，后者通常具有较小的拷贝数，并且映射到不同的位置。这些结果表明，前者克隆主要由多基因家族组成，后者包含转座子。我们现在正在分析这些克隆的详细分子结构。

项目成果

Harada,K.: "Comparative of P element activity in two natural populations of Drosophila melanogaster." Japanese Journal of Genetics. 66. 725-737 (1991)

Harada,K.：“果蝇两个自然群体中 P 元素活性的比较。”

Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

Harada,K.、Kusakabe,S.、Yamazaki,T. 和 Mukai,T：“果蝇酶位点无效突变和带状突变的自发突变率。”

Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T.: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

Harada,K.、Kusakabe,S.、Yamazaki,T. 和 Mukai,T.：“果蝇酶位点无效突变和带状突变的自发突变率。”

The Synchronized Efforts to Decipher the Molecular Basis for Soybean Maturity Loci E1, E2, and E3 That Regulate Flowering and Maturity

同步努力破译调节开花和成熟的大豆成熟位点 E1、E2 和 E3 的分子基础

• DOI: 10.3389/fpls.2021.632754
• 发表时间: 2021
• 期刊: Frontiers in Plant Science
• 影响因子: 5.6
• 作者: Xia Z;Zhai H;Wu H;Xu K;Watanabe S;Harada K
• 通讯作者: Harada K

Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T.: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

Harada,K.、Kusakabe,S.、Yamazaki,T. 和 Mukai,T.：“果蝇酶位点无效突变和带状突变的自发突变率。”