Genetic oncological and molecular epidemiological research on development and progression of human bile duct cancer in Asia

亚洲人类胆管癌发生和进展的遗传肿瘤学和分子流行病学研究

基本信息

批准号：
15406008
负责人：
MIWA Masanao
金额：
$ 6.14万
依托单位：
Nagahama Institute of Bio-Science and Technology (2005)University of Tsukuba (2003-2004)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Taking advantage of collaboration with Dr.Petcharin Srivatanakul of National Cancer Institute of Thailand, we have successful cooperation with doctors and paramedical staffs in Nakorn Phanom Province, North East Thailand, and could perform a population-based case-control study of cholangiocarcinoma, in which sex, age and place of residence were matched individually. We could collect the peripheral blood and the information on the life-style related factors from the individuals who gave the informed consent.We could confirm the liver fluke infection as the risk factor. In addition we found the drinking alcohol is another independent risk factor for cholangiocarcinoma. Past and current regular drinkers of alcohol had higher risk (odds ratio=5.39[95% CI:1.11-26.06] and 4.82[95% CI:1.29-18.06], respectively). Compared to subjects who had a normal antibody range and the wild type GSTM1 gene, those who had elevated antibodies had higher odds rations of 10.34(95% CI:1.31-81.63) for wild type GSTM1 and 18.00(95% CI:3.33-97.40) for the null variant thereof, respectively. We would like to collect more samples for the case-control study to confirm the above results in the near future.On the biological characteristics of cholangiocarcinoma, we established a cholangiocarcinoma cell line, KKU 100, found that the excretory/secretory products from the liver fluke, Opisthorchis viverrini, could increase the cell proliferation of NIH-3T3 cells, and that MUC1 gene expression may be useful for predicting the poor outcome of the patients with intrahepatic cholangiocarcinoma.In addition we also established a gall bladder cancer cell line and found that MUC1 gene expression as a prognostic factor in the patients with gall bladder cancer.

利用与泰国国家癌症研究所的Petcharin Srivatanakul博士合作，我们与泰国东北部Nakorn Phanom省的医生和护理人员进行了成功的合作，可以在哪些性别，性别和生活中匹配的性别，与人口的胆管癌研究中，可以进行基于人群的病例对照研究。我们可以收集外围血液以及给予知情同意的个人的有关生活方式相关因素的信息。我们可以确认肝氟化物感染是危险因素。此外，我们发现饮酒是胆管癌的另一个独立危险因素。过去和当前的常规饮酒者的风险较高（优势比= 5.39 [95％CI：1.11-26.06]和4.82 [95％CI：1.29-18.06]）。与具有正常抗体范围和野生型GSTM1基因的受试者相比，抗体升高的抗体较高的几率为10.34（95％CI：1.31-81.63），野生型GSTM1和18.00（95％CI：95％CI：3.33-97.40）对于null null varient上，抗体的几率较高。我们想为病例对照研究收集更多样品，以在不久的将来确认上述结果。在胆管癌的生物学特征下，我们建立了胆管癌细胞系KKU 100，KKU 100，发现从肝脏中的肝癌，niH-niH-nih the-uc the-uc the-uctrififertifrine-uctrififertiftriftiftiftifififififififififififififififififififififififififtifififtifififififififififififife。可能有助于预测肝内胆管癌患者的不良预后。此外，我们还建立了胆囊癌细胞系，发现MUC1基因表达是胆囊癌患者的预后因素。