Early Diagnosis System of Cartilage Degeneration by Ultrasound

超声软骨退变早期诊断系统

基本信息

批准号：
15390457
负责人：
IKEUCHI Ken
金额：
$ 4.03万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.Ultrasonic system was established to measure articular cartilage using wavelet transform of reflected wave.2.It is found that strength of reflected wave indicates dynamic stiffness while endurance time indicates damage in the cartilage by simultaneous measurements of dynamic stiffness, density, sound velocity and defects in the cartilage.3.It is found that dynamic stiffness, sound velocity and density reduce with a development of degeneration. Thereafter, endurance time of the reflected wave increases and internal reflection appears.4.It is confirmed by experiment with articular cartilage specimens degenerated by use of collagenase enzyme that maximum strength of the reflected wave decreases with a development of degeneration.5.By means of ultrasonic measurement for unstable rabbit knee model by cutting of ligaments and meniscus and for degenerated cartilage model by collagenase treatment, cartilage at medial femoral chondyle degenerates at first and then degeneration appears at lateral femoral chondyle.6.In addition to the transducer of piezo-electric ceramic, transducer of piezo-electric polymer was produced. Both types were prove applicable to diagnoses of knee joint, hip joint, finger joint and vertebra.7.Cartilage surface of transplanted bone-cartilage complex was measured. It is found that regenerated cartilage after transplantation survives during daily life though its dynamic stiffness is lower than the original cartilage.8.Dynamic stiffness of rabbit knee articular cartilage cultured in vitro was significantly lower than the original cartilage.9.Rabbit knee articular cartilages regenerated by means of several regeneration therapies were measured and compared. According to the result, bone marrows induced to the defect by drilling and chondrocytes injected to scaffold produced cartilage with high dynamic stiffness.

1.建立了超声波系统，利用反射波的小波变换测量关节软骨。2.通过同时测量动态刚度、密度、声速和关节软骨，发现反射波的强度指示动态刚度，而持久时间指示软骨的损伤情况。 3.发现随着退变的发展，动态刚度、声速和密度降低。此后，反射波的持续时间增加并出现内反射。4.通过使用胶原酶退化的关节软骨样本的实验证实，反射波的最大强度随着退化的发展而降低。5.通过超声测量韧带、半月板切割不稳定兔膝关节模型和胶原酶处理退变软骨模型，股骨内侧软骨先发生退变，股骨外侧出现退变6.除了压电陶瓷换能器外，还生产了压电聚合物换能器。两种类型均被证明适用于膝关节、髋关节、指关节和椎骨的诊断。7.测量移植骨软骨复合体的软骨表面。结果发现，移植后的再生软骨虽然动态刚度低于原始软骨，但在日常生活中仍能存活。8.体外培养的兔膝关节软骨的动态刚度明显低于原始软骨。9.再生兔膝关节软骨对几种再生疗法的方法进行了测量和比较。根据结果，通过钻孔诱导骨髓缺损，并将软骨细胞注射到支架中，产生具有高动态刚度的软骨。