Isokinetic Closed Kinetic Chain Exercise using Electric Muscle Stimulation

使用电肌肉刺激的等速闭合动力链运动

• 批准号: 15300208
• 负责人: KAWAMURA Kenji
• 金额: $ 7.49万
• 依托单位: KIBI International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300208/
• 关键词: electrical stimulation; closed kinetic chain; muscle contraction; muscle training; neural cell culture; neurite outgrowth; rehabilitation; PC12 cell

1.Isokinetic closed kinetic chain exercise using electric muscle stimulationA new isokinetic testing machine was developed for CKC exercises. With this machine, subjects can move their legs in gradual arc of motions. A custom made foot plate and load cell were attached to the tip of dynamometer arm. When rectus femoris muscle was stimulated by the electric muscle stimulator, the output was displaced in a tiptoe direction. When vastus medialis and vastus lateralis were stimulated by the electric muscle stimulator, the output direction did not change. When hamstring muscle was stimulated by the electric muscle stimulator, the output was displaced in a calx direction. It is speculated that bi-articular muscle contributes to control of an output direction. On the other hand, mono-articular muscle do not contribute to control of an output direction. It is speculated that mono-articular muscle contributes to an absolute value of output force.2.Electrical stimulation induces neurite outgrowth in PC12m3 cells via the p38 MAP kinase pathwayWe developed a neural cell line PC12m3 which show neurite outgrowth by various physical stimuli. When cultures of the PC12 mutant (PC12m3) cells were exposed to electrical stimulation, activity of p38 MAP kinase increased and neurite outgrowth was greatly enhanced. The neurite extension was inhibited by the p38 MAP kinase inhibitor BS203580. Longer electrical stimulation of PC12m3 cells provoked cell death, which was enhanced by SB203580. Furthermore, we detected activation of cyclic AMP responsive element binding protein (CREB) by electrical stimulation in PC12m3 cells. CREB is a transcription factor that is the target of ERK. These findings suggest that electrical stimulation induce neurite outgrowth through p38 MAP kinase and CREB pathways in PC12m3 cells and that p38 MAP kinase is responsible for the survival of neuronal cells.

1.利用肌肉电刺激的等速闭动力链练习针对CKC练习开发了新型等速测试机。使用这台机器，受试者可以以逐渐的弧线运动来移动他们的腿。将定制的脚踏板和称重传感器连接到测力计臂的尖端。当电动肌肉刺激器刺激股直肌时，输出沿脚尖方向位移。当电动肌肉刺激器刺激股内侧肌和股外侧肌时，输出方向没有改变。当肌肉电刺激器刺激腘绳肌时，输出会沿 Calx 方向移动。据推测，双关节肌有助于控制输出方向。另一方面，单关节肌肉对输出方向的控制没有贡献。据推测，单关节肌肉对输出力的绝对值有贡献。2.电刺激通过p38 MAP激酶途径诱导PC12m3细胞中的神经突生长我们开发了神经细胞系PC12m3，其在各种物理刺激下表现出神经突生长。当 PC12 突变体 (PC12m3) 细胞的培养物受到电刺激时，p38 MAP 激酶的活性增加，神经突生长大大增强。 p38 MAP 激酶抑制剂 BS203580 抑制神经突延伸。对 PC12m3 细胞进行较长时间的电刺激会引发细胞死亡，SB203580 会增强这种死亡。此外，我们通过电刺激 PC12m3 细胞检测到环 AMP 反应元件结合蛋白 (CREB) 的激活。 CREB ​​是一种转录因子，是 ERK 的靶标。这些发现表明，电刺激通过 PC12m3 细胞中的 p38 MAP 激酶和 CREB ​​途径诱导神经突生长，并且 p38 MAP 激酶负责神经元细胞的存活。

项目成果

足部がサイクロイド曲線を描くIsokinetic Closed Kinetic Chain Exerciseにおける膝関節反力の筋骨格モデル解析

足部形成摆线的等速闭合动力链运动中膝关节反作用力的肌肉骨骼模型分析

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻8号
• 作者: 松尾高行;河村顕治 他;河村顕治 他
• 通讯作者: 河村顕治 他

前十字靭帯損傷膝における開運動連鎖及び閉運動連鎖による下肢筋力評価

使用开放式和封闭式动力链评估前交叉韧带损伤膝关节下肢肌力

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻2号
• 作者: 阿部信寛;河村顕治 他
• 通讯作者: 河村顕治 他

足部がサイクロイド曲線を描く Isokinetic Closed Kinetic Chain Exercise における膝関節反カの筋骨格モデル解析

足部形成摆线的等速闭合运动链运动中膝关节受力的肌肉骨骼模型分析

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻 8号
• 作者: 松尾高行;河村顕治 他;河村顕治 他
• 通讯作者: 河村顕治 他

Effect of contralateral hip flexion on the muscle recruitment patterns during isometric closed kinetic chain exercise

等长闭合动力链运动期间对侧髋关节屈曲对肌肉募集模式的影响

• 发表时间: 2004
• 期刊: Japanese Journal of Clinical Biomechanics Vol.25
• 作者: Kenji KAWAMURA;et al.
• 通讯作者: et al.

Knee Rehabilitation with Open Kinetic Chain and Closed Kinetic Chain Exercise in ACL Injury

开放式运动链和封闭式运动链训练在 ACL 损伤中的膝关节康复

• 发表时间: 2004
• 期刊: J.Jpn.Orthop.Assoc. 78(4)
• 作者: Nobuhiro ABE;Kenji KAWAMURA;et al.
• 通讯作者: et al.