ADP-ribosyl cyclase coupled with dopamine receptors : Application to Parkinson's disease

ADP-核糖基环化酶与多巴胺受体偶联：在帕金森病中的应用

• 批准号: 15300124
• 负责人: HIGASHIDA Haruhiro
• 金额: $ 9.09万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300124/
• 关键词: Cyclic ADP-ribose; CD38; NAD^+; NG108-15 cells; Striatum; Dopamine; Dopamine receptor

We examined the role of cyclic ADP-ribose (cADPR) as a second messenger downstream of receptors in the striatal cortex and NG108-15 cells. To address this question, ADP-ribosyl cyclase activity was measured in a crude membrane fraction of rat striatum and NG108-15 cells transfected with CD38. Dopamine (DA) stimulated the cyclase activity. The cyclase activity in overexpressed cells had various effects on neuronal function. The following two reports are the summary of the cADPR function written in a review form (7-2) and research report on CD38 transfection (7-3)

我们研究了环状 ADP-核糖 (cADPR) 作为纹状体皮质和 NG108-15 细胞中受体下游的第二信使的作用。为了解决这个问题，在大鼠纹状体和用 CD38 转染的 NG108-15 细胞的粗膜部分中测量了 ADP-核糖基环化酶活性。多巴胺（DA）刺激环化酶活性。过表达细胞中的环化酶活性对神经元功能有多种影响。以下两篇报告分别是写在综述表中的cADPR功能总结（7-2）和CD38转染的研究报告（7-3）

项目成果

Acetylcholine stimulates cyclic ADP-ribose formation via M1 muscarinic receptors in rat superior cervical ganglion.

乙酰胆碱通过大鼠颈上神经节中的 M1 毒蕈碱受体刺激环状 ADP-核糖的形成。

• 发表时间: 2005
• 期刊: Biochem Biophys Res Commun. 335
• 作者: Zhang JS;Jin D;Higashida H.
• 通讯作者: Higashida H.

Higashida, H., Zhang, JS, Mochida, S., Noda, M., Hoshi, N., Hashii, M., Yokoyama, S.et al.: "Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells"J.Neurochem.. 85.

Higashida, H.、Zhang, JS、Mochida, S.、Noda, M.、Hoshi, N.、Hashii, M.、Yokoyama, S.等人：“与代谢型谷氨酸的 ADP-核糖基环化酶的亚型特异性偶联

パーキンソン病の治療のための医薬

治疗帕金森病的药物

• 发表时间: 2005

Acetylcholine stimulates cyclic ADP-ribose formation via Ml muscarinic receptors in rat superior cervical ganglion.

乙酰胆碱通过大鼠颈上神经节中的M1毒蕈碱受体刺激环状ADP-核糖形成。

• 发表时间: 2005
• 期刊: Biochem Biophys Res Commun. 335
• 作者: Zhang JS;Jin D;Higashida H.
• 通讯作者: Higashida H.

Yamaguchi, M., Shimizu, M., Ino, H., Terai, K., Hoshi, N., Higashida, H.: "Clinical and electrophysiological characterization of a novel mutation (F193L) in the KCNQ1 gene associated with long QT syndrome"Clinical Science. 104. 377-382 (2003)

Yamaguchi, M.、Shimizu, M.、Ino, H.、Terai, K.、Hoshi, N.、Higashida, H.：“与长 QT 相关的 KCNQ1 基因中的新突变 (F193L) 的临床和电生理学特征