Studies on bilirubin metabolism in fish

鱼类胆红素代谢的研究

• 批准号: 02660217
• 负责人: SAKAI Tadashi
• 金额: $ 1.22万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660217/
• 关键词: Jaundice; alpha-Tocoherol; Anti-bilirubin monoclonal anitibody; Bilirubin; In vivo lipid peroxidation; Brown fish meal; Antioxidant; Bile pigments; 黄疸

1. TBA values, content of alpha-Toc in some tissues of jaundiced yellowtail were analyzed. Bilirubin contents in the plasma of jaundiced yellowtail were analyzed by HPLC and EIA method. In vivh lipid peroxidadon progressed in the tissues of jaundiced fish and so call blirubin X increased in the plasma. These results confirm that the active oxygen species may be a piincipale cause of the jaundice.2. Yellowtail were fed on three brown fish meal diets whose lipid peroxide levels were deffemt (group I-111) or brown fishmeal 9 % of sardine oil whose POV was 4.7 (group IV) or 100 (group V) for 30 days and changes in a-tocopherol (alpha-Toc) contents, 2-thiobarbituric acid reactive substances (TBARS) values and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver were analyzed. In vivo lipid peroxidaion may progress in the liver of yellowtail fed on diets whose lipid peroxidatin was the highest levels or which contain lipid peroxides and that the suppression of in vivo lipid peroxidation may prevent the jaundice of yellowtail.3. Highperbilirubinemia were observed in red sea bream obtained from fish farm in April and June, 1989. The cause may probably related to the reproduction.4. Seasonal changes of in vivo lipid peroxidation and free radical scavenging abilty of yellowtail were analyzed. Results obtained account for the reason that the jaundice of yellowtail occurred mainly in summer.5. Anti-bilirubin-lX alpha monoclonal antibodies exclusively specific for unconjugated bilirubin-lX alpha are prepared and characterized. The antibodies reacted only bilirubin-IX alpha but did not react with conjugated bilirubin-lX alpha.6. Bilirubin contents in the serum of several disease were analyzed by diazo method and EIA method in which antibilirubin-lX alpha monoclonal antibodies were used. Structure of so call bilirubin X were also studied. Results obtained indicate that bilirubin is the most primitive and effective free radical scavenger.

1.分析黄尾鰤部分组织的TBA值、α-Toc含量。采用HPLC和EIA法对黄鰤血浆中胆红素含量进行分析。黄疸鱼体内的脂质过氧化物在体内进展，血浆中所谓的胆红素X增加。这些结果证实活性氧可能是黄疸的主要原因。 2．黄尾鱼喂食三种棕色鱼粉饲料，其过氧化脂质水平为defem（I-111组）或棕色鱼粉9％的沙丁鱼油，其POV为4.7（IV组）或100（V组）30天，并改变-生育酚（α-Toc）含量、2-硫代巴比妥酸反应物质（TBARS）值和超氧化物歧化酶（SOD）和分析肝脏中谷胱甘肽过氧化物酶（GSH-Px）的活性。饲喂脂质过氧化素含量最高或含有脂质过氧化物的饲料时，黄尾鱼肝脏可能发生体内脂质过氧化反应，抑制体内脂质过氧化可预防黄尾鱼的黄疸。 3． 1989年4月、6月从养鱼场获得的红鲷鱼出现高胆红素血症，其原因可能与繁殖有关。 4．分析了鰤鱼体内脂质过氧化和自由基清除能力的季节变化。结果表明，黄尾鱼的黄疸主要发生在夏季。 5．制备并表征了专门针对未结合的胆红素-1X α的抗胆红素-1X α单克隆抗体。抗体仅与胆红素-IX α反应，但不与缀合胆红素-IX α.6反应。采用重氮法和使用抗胆红素-1Xα单克隆抗体的EIA法分析了几种疾病血清中的胆红素含量。还研究了所谓的胆红素 X 的结构。所得结果表明胆红素是最原始、最有效的自由基清除剂。