Microbial community analysis for evaluating water quality improvement in reservoirs installed with destratification system

微生物群落分析评价安装去层系统水库水质改善情况

• 批准号: 13680638
• 负责人: FURUMAI Hiroaki
• 金额: $ 2.3万
• 依托单位: the University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680638/
• 关键词: eutrophication; algal bloom; destratification system; eubacteria; community analysis; PCR-DGGE; Microcystis; water reservoir

Seasonal change of microbial community including bacteria and algae in Tsukui Lake, where destratification system was installed, was investigated by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR). In order to express graphically the seasonal change of the community based on the dissimilarity index, multi-dimensional scaling (MDS) was applied. The MDS map revealed a cyclic change of the community and a distinctive divergence from the path during a sudden algal bloom after a heavy storm. Since the divergence disappeared in an analysis excluding cyanobacteria, the major part of the community might be independent of the sudden algal bloom. Dissimilarity of microbial communities was much lower among three different sampling sites than among the sampling months.Excitation emission matrix spectroscopy (EEMS) and PCR-DGGE were applied to elucidate the relationship between production of dissolved organic matter (DOM) and bacterial community change during the l … More ysis of algal bloom followed by their degradation in a batch incubation test. The lysis of the algal bloom collected from Tsukui Lake was indicated by rapid increases of dissolved organic carbon and UV absorbance at 260 nm within five days In accordance with the lysis, several fluorescence peaks derived from humic- and protein-like substances appeared in the EEM. Phylogenetic analysis of the major DGGE bands showed that bacteria closely related to y-Proteobacteria, CFB group and a-Proteobacteria probably played important roles in the lysis and degradation of algal bloom.The phenomena of algal lysis and DOM formation were investigated also in laboratory. Pure culture of Microcystis aeruginosa was inoculated into sterilized and non-sterilized Tsukui lake water. The existence of eubacteria differed the DOM formation after the algal lysis. The fluorescence derived from humic-like substances increased and subsequently decreased in the non-sterilized lake water, while such a variation was not observed in the sterilized water. Less

通过聚合酶链式反应（PCR）的变性梯度凝胶电泳（DGGE）对安装了去层系统的津久井湖中细菌和藻类等微生物群落的季节变化进行了研究，以便基于图形表达群落的季节变化。应用相异指数、多维尺度（MDS），MDS图揭示了群落的周期性变化，以及在暴风雨后突然藻华期间与路径的明显分歧。在排除蓝藻的分析中，分歧消失了，群落的主要部分可能与突然的藻华无关。三个不同采样点之间的微生物群落差异比采样月份之间要低得多。激发发射矩阵光谱（EEMS）和PCR-DGGE 用于阐明藻华裂解过程中溶解有机物 (DOM) 的产生与细菌群落变化之间的关系，随后在批量孵化测试中进行降解。从筑井湖收集的藻华的变化表现为五天内溶解的有机碳和 260 nm 处的紫外线吸光度的快速增加。根据裂解情况，EEM 中出现了几个来自腐殖质和类蛋白质物质的荧光峰。对DGGE主要条带的分析表明，与y-Proteobacteria、CFB群和a-Proteobacteria密切相关的细菌可能在藻华的裂解和降解过程中发挥了重要作用。还在实验室中研究了铜绿微囊藻的纯培养物接种到灭菌和未灭菌的筑井湖水中，真细菌的存在使藻类溶解后的 DOM 形成有所不同。随后在未消毒的湖水中减少，而在消毒的水中没有观察到这种变化。

项目成果

春日郁朗: "DGGE法およびクローニング法によるアオコ分解過程における真正細菌群集の構造解析"第18回日本微生物生態学会講演要旨集. 58 (2002)

春日郁郎：“利用DGGE法和克隆法对蓝绿藻分解过程中的真细菌群落进行结构分析”第18届日本微生物生态学会会刊58（2002）。

Ikuro Kasuga, Hiroaki Furumai, Fumiyuki Nakajima and Suzue Arii: "Relationship between occurrence of algal bloom and the behavior of PCR-DGGE band pattern"Proceedings of 4th symposium of Japan Society on Water Environment. 29-30 (2001)

Ikuro Kasuga、Hiroaki Furumai、Fumiyuki Nakajima、Suzue Arii：“藻华发生与PCR-DGGE带模式行为之间的关系”日本水环境学会第四次研讨会论文集。

Ikuro Kasuga, Fumiyuki Nakajima and Hiroaki Furumai: "Analysis of bacterial community in degradation of algal bloom by DGGE and cloning methods"Proceedings of 18th annual conference of Japanese Society of Microbial Ecology. 58 (2002)

Ikuro Kasuga、Fumiyuki Nakajima 和 Hiroaki Furumai：“通过 DGGE 和克隆方法分析藻华降解过程中的细菌群落”第 18 届日本微生物生态学会年会论文集。

P.B. Thapa, F. Nakajima and H. Furumai: "Characterization of natural organic matter in a shallow eutrophic lake"Water Science and Technology. 46, No.11-12. 465-471 (2002)

PB

春日郁朗: "DGGE法およびクローニング法によるアオコ分解過程における真正最近群集の構造解析"第18回日本微生物生態学会講演要旨集. 58 (2002)

Ikuro Kasuga：“使用 DGGE 方法和克隆方法对藻类分解过程中真实的近期群落进行结构分析”第 18 届日本微生物生态学会会议记录 58 (2002)。