Effects of endurance training with hypoxic stimulation induced by Co^<2+> treatment on oxygen transport system in rat skeletal muscles

Co^2处理低氧刺激耐力训练对大鼠骨骼肌氧运输系统的影响

基本信息

批准号：
13680011
负责人：
SUZUKI Junichi
金额：
$ 1.92万
依托单位：
Hokkaido University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680011/
关键词：
angiogenesis training hypoxia cobalt enzyme activity skeletal muscle endurance capacity 酸素活性

项目摘要

This study was designed to examine the changes in the capillary geometry and expression of angiogenic growth factor in skeletal muscles after endurance training with and without chronic CoCl_2 administration. The Co^<2+>-treated rats drank water containing 0.01% CoCl_2. In the first experiment, training and Co^<2+> administration lasted for 5 weeks. Chronic Co^<2+> administration significantly increased the blood hemoglobin concentration by approximately 25% with and without training. Only in the diaphragm, the Co^<2+> treatment alone significantly increased total capillary density and the capillary-to-fiber ratio (C:F). Both training groups with and without Co^<2+> administration significantly increased the C:F in soleus (SOL) and plantaris (PL) muscles. In PL, the increase was significantly greater in the Co^<2+>-treated training group than in the non-Co^<2+>-treated training group. The densities of VEGF-positive capillaries (VEGF-cap) remained unchanged in all muscle portions examin … More ed after either Co^<2+> administration or exercise training. The second experiment was designed to examine the time course changes in VEGF expression and capillary geometry in the early stage of the treatment. After 6 and 10 days of the Co^<2+> administration, the VEGF-cap was significantly increased by 27% and 65%, respectively in SOL. After 10 days of Co^<2+> administration, the C:F was significantly increased. The training with Co^<2+> significantly increased the VEGF-cap by 69%, 44% and 60%, respectively, after 3, 6 and 10 days in SOL, while it significantly increased the VEGF-cap after 10 days in the mixed fiber portion of plantaris muscle (PLm). Training with or without Co^<2+> significantly increased the C:F after 6 and 10 days in the SOL. Training with Co^<2+>, but training alone did not, significantly increased the C:F in PLm after 10 days. In condition, the present results suggest that activation of the cellular oxygen-sensing mechanism induced by Co^<2+> administration facilitates microvascular remodeling in hind-leg muscles by facilitating an expression of VEGF. Less

本研究旨在检查在有或没有长期给予CoCl_2 的耐力训练后骨骼肌中毛细血管几何形状和血管生成生长因子表达的变化。Co ^ 2+ 处理的大鼠饮用含有0.01％CoCl_2 的水。第一个实验，训练和Co 2+ 施用持续5周，无论是否进行训练，慢性Co 2+ 施用均使血液血红蛋白浓度显着增加约25％。在膈肌中，单独使用Co ^ 2+ 治疗显着增加了总毛细血管密度和毛细血管与纤维的比率(C:F)，使用和不使用Co ^ 2+ 施用的两个训练组都显着增加了C：比目鱼肌(SOL)和跖肌(PL)中的F 在PL中，Co ^ 2+ 处理的训练组中的增加显着大于非Co ^ 2+ 处理的训练组。密度在 Co^2+ 给药或运动训练后，所有肌肉部分的 VEGF 阳性毛细血管 (VEGF-cap) 保持不变。第二个实验旨在检查 VEGF 表达和毛细血管几何形状的时间过程变化。在治疗的早期阶段，在Co ^ 2+ 施用6天和10天后，SOL中的VEGF-cap分别显着增加27％和65％。 Co ^ 2+ 施用后，C:F显着增加，Co ^ 2+ 训练在3、6和10后分别使VEGF-cap显着增加69％、44％和60％。在SOL中，10天后，跖肌混合纤维部分(PLm)中的VEGF-cap显着增加，有或没有Co^2+的训练在6天后显着增加了C:F。在SOL中训练10天，但单独训练并没有显着增加10天后PLm中的C:F，目前的结果表明细胞氧传感机制的激活。 Co 2+ 施用通过促进VEGF的表达而促进后腿肌肉中的微血管重塑。