Risk assessment of peri-inplantitis patients with chair-side MMP-8 assay system

椅旁MMP-8检测系统对种植体周围炎患者的风险评估

基本信息

批准号：
13672031
负责人：
ARAKAWA Hikaru
金额：
$ 2.56万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Matrix metalloproteinases (MMPs) are known to play an important role in pathological tissue destruction in the periodontal tissues. Therefore, MMPs related to bone matrix degradation could be a possible marker for ongoing bone destruction around the peri-implantitis implants. In this study, four peri-implantitis patients (male/female : 2/2 ; mean age : 68.5^^+__-6.7 yrs ; 9 implants ) and age- and sex- matched four control patients (male/female : 2/2, mean age : 66.0^^+__-5.8 yrs, 8 implants) were selected from 64 consecutive patients who were installed implant-supported superstructures in our school from 1990 to 2000. Inclusion criteria for selection of the peri-implantitis and control patients in this study were having an implant "with accumulated vertical bone loss (ABL) of more than 0.6 mm per year" and "with ABL of less than 0.2 mm per year" after superstructure installation, respectively. Bone levels around implants were assessed by dental x-ray films taken annually and mean valu … More es of three calibrated observers readings were used as outcome. Ongoing vertical bone loss per year (OBL) after superstructure installation was also calculated. Gingival crevicular fluids (GCFs) were collected at the mesio-buccal comer of the gingival crevices by using absorbent paper points. Then the samples were dissolved in 0.1% phoshate buffered saline with Tween 20 (PBS-T) and stored at -20℃. MMPs in GCFs were detected by Western blotting using anti-human monoclonal antibodies specific for MMP-1, -8 and -13 (Fuji Chemical Industry, Japan) with positive controls. As the results, in the peri-implantitis patients, MMP-8 was detected in three actively bone-resorbing implants whose OBL at GCF sampling was more than 0.8 mm and not detected in the other six implants whose OBL at GCF sampling was less than 0.3 mm, while no MMP-8 was detected in the controls. MMP-1 and -13 could not be detected in any GCFs in this study. These results suggest that MMP-8 might be a marker for prediction of ongoing bone destruction around the peri-implantitis implants. Less

已知基质金属蛋白酶 (MMP) 在牙周组织的病理组织破坏中发挥重要作用，因此，与骨基质降解相关的 MMP 可能是种植体周围炎种植体周围持续骨破坏的可能标志。种植体周围炎患者（男性/女性：2/2；平均年龄：68.5^^+__-6.7 岁；9 个种植体）以及年龄和性别匹配的 4 名对照患者（男/女：2/2，平均年龄：66.0^^+__-5.8 岁，8 个种植体）是从 1990 年至 2000 年期间在我们学校安装种植体支撑上层结构的 64 名连续患者中选出的。本研究中选择种植体周围炎患者和对照患者的标准是种植体“累积垂直骨丢失 (ABL) 超过 0.6 毫米/次”上部结构安装后，分别为“每年”和“每年 ABL 小于 0.2 毫米”。种植体周围的骨水平通过每年拍摄的牙科 X 光片进行评估，并使用三个校准观察者读数的平均值作为结果。还计算了上部结构安装后每年持续的垂直骨丢失（OBL），并通过在牙龈缝隙的近颊角处收集龈沟液（GCF）。然后将样品溶解在含有 Tween 20 (PBS-T) 的 0.1% 缓冲盐水中，并保存在 GCF 中，使用针对 MMP-1 的抗人单克隆抗体进行蛋白质印迹检测。、-8和-13（日本富士化学工业）与阳性对照结果显示，在种植体周围炎患者中，3名活跃患者中检测到了MMP-8。骨吸收种植体的 GCF 取样时的 OBL 大于 0.8 毫米，而在其他 6 种 GCF 取样时的 OBL 小于 0.3 毫米的种植体中未检测到，而对照中未检测到 MMP-1 和 -13。在本研究中，在任何 GCF 中均未检测到 MMP-8，这些结果表明 MMP-8 可能是预测种植体周围炎种植体周围持续骨质破坏的标志物。