The role of tissue factor on lung cancer metastasis

组织因子在肺癌转移中的作用

• 批准号: 13671371
• 负责人: MINAMIYA Yoshihiro
• 金额: $ 2.56万
• 依托单位: AKITA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671371/
• 关键词: non-small lung cancer; tissue factor; metastasis; inavasion; blood vessel; lymphatic vessel; 血管浸潤

Purpose; Tissue factor (TF), an initiator of the extrinsic coagulation cascade, is also expressed in a wide range of cancer cells and plays important roles in cancer progression, and metastasis, processes independent of the blood coagulation pathway. For example, by acting as an adhesion molecule enabling tissue invasion, TF may play a key role in the metastatic process and angiogenesis in non-small cell lung cancer (NSCLC). Methods: To further investigate the role of TF on tumor cell invasion in NSCLC, we measured TF mRNA expression in the tumors of 42 NSCLC patients using real time quantitative reverse transcription polymerase chain reaction carried out in a LightCycIer. We then compared TF mRNA expression with the histological evidence of invasion of blood and lymphatic vessels by tumor cells. Results: Although, there was no significant relationship between TF mRNA expression and invasion of lymphatic vessels, TF mRNA expression was significantly higher in tumors that invaded blood vessels (Log_<10 >TF mRNA/GAPDH mRNA = 2.16 ± 0.18) than in those that did not (1.59 ± 0.16, P = 0.03). Conclusion: These results suggest that TF plays a major role in blood vessel invasion by tumor cells in NSCLC.

目的；组织因子 (TF) 是外源性凝血级联的引发剂，也在多种癌细胞中表达，并在癌症进展和转移等独立于凝血途径的过程中发挥重要作用。 TF作为一种能够实现组织侵袭的粘附分子，可能在非小细胞肺癌（NSCLC）的转移过程和血管生成中发挥关键作用。方法：进一步研究TF对肿瘤的作用。在 NSCLC 细胞侵袭中，我们使用 LightCycIer 中进行的实时逆转录聚合酶链反应测量了 42 名 NSCLC 患者肿瘤中的 TF mRNA 表达，然后我们将 TF mRNA 表达与血管和淋巴管侵袭的组织学证据进行比较。结果：虽然TF mRNA表达与淋巴管侵袭无显着关系，但侵袭血管的肿瘤中TF mRNA表达显着升高（Log_<10>TF）。 mRNA/GAPDH mRNA = 2.16 ± 0.18）比没有的组（1.59 ± 0.16，P = 0.03）结论：这些结果表明 TF 在 NSCLC 肿瘤细胞的血管侵袭中发挥着重要作用。