Gene expression profile analysis of regenerating liver after portal vein ligation in rats by a cDNA microarray system

cDNA微阵列系统分析大鼠门静脉结扎后再生肝脏的基因表达谱

• 批准号: 13671324
• 负责人: SHIMADA Hiroshi
• 金额: $ 1.41万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671324/
• 关键词: liver regeneration; portal vein embolization; DNA microarray

Aims : We assessed changes in gene expression of hypertrophied liver after portal vein ligation (PL) in a test group of rats compared to a control group which had the same size liver but no PL.Methods : The portal veins of the left and median lobes in the test group were ligated in an initial operation. Four days after the PL, the liver volume of the posterior caudate lobe (5%) increased two-fold and comprised 10% of the liver. A 90% hepatectomy was then performed, leaving only the hypertrophied posterior caudate lobe, and leaving the normal anterior and posterior caudate lobes (10%) in the control (sham) group. A comparison of the expression profiles between two groups was performed using cDNA microarrays. And the hepatic ATP level was measured.Results : The survival rate for the PL group was significantly higher than for the sham group at 4 days after the hepatectomy (56.3% and 26.7%, p<0.05). Gene expression of cyclin D1, PCNA, cyclin A and B was up-regulated, and the CDK inhibitor was down-regulated. Increases were observed in : (i) pyruvate dehydrogenase, the TCA cycle regulator, (ii) acyl-CoA dehydrogenase, the oxidation regulator, and (iii) cytochrome oxidases, the oxidative phosphorylation regulator. Hepatic ATP concentration after hepatectomy was better maintained in the PL group than in the sham group (0.48±0.01μmol/ml vs. 0.33±0.01μmol./ml, p<0.05).Conclusion : The regenerating liver increased tolerance for extended hepatectomy compared to normal liver. It is believed that this is because the induced rapid regeneration of the remaining liver after hepatectomy increases ATP metabolism. Key words : liver regeneration, portal vein embolization, extended hepatectomy, DNA microarray, hepatic ATP level.

目的：与具有相同尺寸肝脏但没有PL的对照组相比，我们评估了一组大鼠的门静脉连接后（PL）的肥大肝脏基因表达的变化：左侧的门静脉和中位数裂片，在初始操作中测试组中的中位数裂片。 PL后四天，后尾状叶的肝脏体积（5％）增加了两倍，并完成了10％的肝脏。然后进行了90％的肝切开术，仅留下肥大的后尾状叶，并在对照组（假）组中留下正常的前尾状裂片（10％）。使用cDNA微阵列对两组之间的表达谱进行比较。测量肝ATP水平。分子：PL组的存活率显着高于肝切开术后4天（56.3％和26.7％，p <0.05）的假手术组的生存率。细胞周期蛋白D1，PCNA，细胞周期蛋白A和B的基因表达被上调，CDK抑制剂被下调。在：（i）丙酮酸脱氢酶，TCA循环调节剂，（ii）酰基-COA脱氢酶，氧化调节剂和（III）细胞色素氧化物，氧化磷酸化调节剂。与假手术组相比，肝切开术后肝ATP浓度更好（0.48±0.01μmol/ml vs. 0.33±0.01μmol。/ml。/ml，p <0.05）。结论：结论：与正常肝脏相比，再生的肝脏对扩展的肝脏的耐受性增加。人们认为这是因为肝切开术后诱导的剩余肝脏的快速再生增加了ATP代谢。关键词：肝脏再生，门静脉栓塞，伸展的肝切开术，DNA微阵列，肝ATP水平。

项目成果

Nagano Y., Shimada H.et al.: "Improve functional reserve of hypertrophied contra lateral liver after portal vein ligation in rats"J. Hepatology. 37(7). 72-77 (2002)

Nagano Y.，Shimada H.et al.：“改善大鼠门静脉结扎后肥大的对侧肝脏的功能储备”J。

Nagano Y, Shimada H et al.: "Gene expression profile analysis of regenerating liver after portal vein ligation in rats by a cDNA microarray system"Liver International. (In Press).

Nagano Y、Shimada H 等人：“通过 cDNA 微阵列系统对大鼠门静脉结扎后再生肝脏进行基因表达谱分析”肝脏国际。

Nagano Y: "Improved functional reserve of hypertrophied contra lateral liver after portal vein ligation in rats"J.Hepatology. 37(7). 72-77 (2002)

Nagano Y：“大鼠门静脉结扎后肥大的对侧肝脏的功能储备得到改善”J.Hepatology。

Nagano Y, Shimada H et al.: "Improved functional reserve of hypertrophied contra lateral liver after portal vein ligation in rats"J.Hepatology. 37(7). 72-77 (2002)

Nagano Y、Shimada H 等人：“大鼠门静脉结扎后肥大的对侧肝脏的功能储备得到改善”J.Hepatology。

Nagano Y: "Gene expression profile analysis of regenerating liver after portal vein ligation in rats by a cDNA microarray system"Liver International. (In press).

Nagano Y：“通过 cDNA 微阵列系统对大鼠门静脉结扎后再生肝脏进行基因表达谱分析”肝脏国际。