Immunohistochemical study of disease-specific immune reaction in inflammatory bowel disease.

炎症性肠病疾病特异性免疫反应的免疫组织化学研究。

• 批准号: 13670545
• 负责人: NAKAMURA Shiro
• 金额: $ 2.18万
• 依托单位: Osaka City University Graduate School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670545/
• 关键词: inflammatory bowel disease; ulcerative colitis; Crohn's disease; lymphocyte; B cell; plasma cell; prolifaration; immunohistochemistry; IBD(炎症性腸疾患); Ki67; basal plasmacytosis; plasmacytosis; 腸間膜リンパ節; 肉芽腫

We analyzed ulcerative colitis from the standpoint of lymphocytic expansion in the loco by immunohistochem- istry and flow cytometry. Ulcerative colitis was character- ized by a diffuse distribution of Ki-67+ small round cells particularly in the ulcer base (that were CD19+ and CD20-), with a significant number of them also CD138+. Immuno- electron microscopy for CD19 revealed an abundance of rough endoplasmic reticulum in the cytoplasm. These in- dicated that they are of immature plasma lineage cells. By contrast, plasma cells in Crohn's disease were negative for CD19, and the labeling for Ki-67 was infrequent, showing mature phenotype. Flow cytometry revealed an occurrence of CD19+ and CD20- cells in ulcerative colitis but not in Crohn's disease. The labeling index of Ki-67 among CD19+ plasma cells was positively correlated with the clinical activity of ulcerative colitis. High labeling of Ki-67 in CD19+ plasma cells is specific for active ulcerative colitis that was resistant to med … More ical treatment by corticosteroids.Next, We investigated he Th1 response and SLC and ELC in Crohn's disease pathogenesis immunohistochemicaly. ELC was almost undetectable in intestinal samples. SLC was found sporadically in lymphoid follicles, lymphoid aggregate venules, and lymphatic vessels. In mesenteric lymph nodes (MLNs), SLC was highly expressed in high endothelial venules (HEVs), lymphatic vessels, and stromal Dendritic cells (DCs), predominantly in T cell areas. ELC was highly expressed in mature DCs. There were significantly more SLC positive HEVs and ELC positive mature DCs, important components of T cell areas, in Crohn's disease. SLC, ELC, and CCR7 mRNA was significantly higher in Crohn's disease MLNs compared with Ulcerative colitis. Crohn's disease MLNs had increased expression of SLC and ELC, mainly in HEVs, mature DCs, and lymphatic vessels, inducing T cell hyperplasia. CCR7 mRNA was increased in T cell areas. The dominant Th1 immune response is facilitated by interaction of SLC positive HEVs/lymphatic vessels, ELC positive mature DCs, and CCR7 positive T cells in hyperplastic T cell areas. In Crohn's disease, memory T cells and mature DCs may home to MLN Less

我们通过免疫组织化学和流式细胞术从局部淋巴细胞扩张的角度分析了溃疡性结肠炎。溃疡性结肠炎的特征是 Ki-67+ 小圆形细胞的弥漫性分布，特别是在溃疡基底部（即 CD19+ 和 CD20 -）。其中相当一部分还具有 CD138+ 的 CD19 免疫电镜显示细胞质中有丰富的粗面内质网。这些表明它们是未成熟的浆细胞系细胞，相比之下，克罗恩病的浆细胞CD19呈阴性，并且Ki-67的标记很少，显示出成熟的表型存在CD19+和CD20-。 CD19+浆细胞中Ki-67的标记指数与溃疡性结肠炎的临床活性呈正相关。 CD19+ 浆细胞中的 Ki-67 标记对于对皮质类固醇药物治疗有抵抗力的活动性溃疡性结肠炎具有特异性。接下来，我们研究了克罗恩病发病机制中的 Th1 反应以及 SLC 和 ELC。 ELC 在肠道中几乎检测不到。样本中，淋巴滤泡、淋巴集合小静脉和肠系膜淋巴结中偶有发现 SLC。 (MLN)、SLC 在高内皮微静脉 (HEV)、淋巴管和基质树突细胞 (DC) 中高表达，主要在 T 细胞区域中高表达，SLC 阳性 HEV 和 ELC 显着增多。与克罗恩病 MLN 相比，克罗恩病 MLN 中的阳性成熟 DC（T 细胞区域的重要组成部分）的 SLC、ELC 和 CCR7 mRNA 显着更高。溃疡性结肠炎 MLN 的 SLC 和 ELC 表达增加，主要在 HEV、成熟 DC 和淋巴管中，诱导 T 细胞区域的 CCR7 mRNA 增加。SLC 的相互作用促进了主导的 Th1 免疫反应。增生性 T 细胞区域中的阳性 HEV/淋巴管、ELC 阳性成熟 DC 和 CCR7 阳性 T 细胞、记忆 T 细胞和成熟 DC。可能是 MLN 的所在地 Less

项目成果

Oshitani N. et al.: "Decreased anti-Saccharomyces cerevisiae antibody titer by mesalazine in patients with Crohn's disease"J Gastroenterol Hepatol. 15. 1400-1403 (2001)

Oshitani N. 等人：“美沙拉秦可降低克罗恩病患者的抗酿酒酵母抗体滴度”J Gastroenterol Hepatol。

Matsumoto T. et al.: "Role of granuloma in the immunopathogenesis of Crohn's disease"Digestion. 13-18 (2001)

Matsumoto T. 等人：“肉芽肿在克罗恩病免疫发病机制中的作用”消化。

Oshitani N. et al.: "Heterogeneity of HPLC profiles of human class II MHC-bound peptides isolated from intestine with inflammatory bowel disease"Dig Dis Sci. Vol.47. 2088-2094 (2002)

Oshitani N. 等人：“从患有炎症性肠病的肠道中分离出的人 II 类 MHC 结合肽的 HPLC 谱的异质性”Dig Dis Sci。

rifiltration of CD19+plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant activeulcerative colitis

皮质类固醇抵抗性活动性溃疡性结肠炎中频繁标记 Ki-67 的 CD19 浆细胞的再过滤

• 发表时间: 2006
• 期刊: Virchows Arch. 448
• 作者: Jinno Y;et al.
• 通讯作者: et al.

クローン病におけるリンパ管と抗原提示細胞の動態に関する免疫組織化学的研究

克罗恩病淋巴管和抗原呈递细胞动态的免疫组织化学研究

• 发表时间: 2000
• 作者: 中村志郎;他
• 通讯作者: 他