Effect of Centrifugal Force on Chondrocytes from Craniofacial Complex

离心力对颅面复合体软骨细胞的影响

• 批准号: 01571118
• 负责人: INOUE Hiroyuki
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571118/
• 关键词: Centrifugation; Mechanical Force; Mandibular chondrocytes; Alkaline phosphatase; Calcification; Nasal septal chondrocyte; アルカリフォスファタ-ゼ; 軟骨細胞; 基質; アルカリンフォスタ-ゼ

To investigate the effect of biomechanical forces on chondrocytes form craniofacial complex, nasal septal and mandibular chondrocytes were maintained in centrifuge tubes as Packed mass. Cells were centrifuged with a centrifuge in a CO_2 incubator. The centrifugation of nasal septal chondrocytes at 27 g caused a two-fold increase in both [^<35>S]sulfate incorporation into GAG and [^3H]thymidine incorporation into DNA under 0.3% serum. On the other hand, the centrifugation of mandibular chondrocytes at 27 g for 24 hours caused a 2-fold increase in [^<35>S]sulfate incorporation into GAG. However, the centrifugation of mandibular chondrocytes at 27 g for 24 hours caused 50% decrease effect on [^3H]thymidine incorporation into DNA. Nasal septal chondrocytes express low ALPase activity in pelleted cultures. However, the centrifugation of nasal septal chondrocytes at 27 g for 23 days under 10% serum caused a ten-fold increase in ALPase activity. Mandibular chondrocytes express high ALPase activity in pelleted cultures. The centrifugation of mandibular chondrocytes at 27 g for 7 days under 10% serum caused a two-fold increase in ALPase activity. These results suggested that biomechanical forces have differential effects on growth in nasal septal and mandibular chondrocytes, and stimulate the differentiation and calcification of chondrocytes in culture.

为了研究生物力学力对颅面复合体软骨细胞的影响，将鼻中隔和下颌软骨细胞作为堆积物保存在离心管中。将细胞在CO_2培养箱中用离心机离心。在0.3％血清下，以27g离心鼻中隔软骨细胞导致GAG中的[^ 35 S]硫酸盐掺入和DNA中的[^ 3H]胸苷掺入增加两倍。另一方面，下颌软骨细胞在27g下离心24小时导致掺入GAG中的[^ 35 S]硫酸盐增加2倍。然而，下颌软骨细胞在27 g下离心24小时导致[^3H]胸苷掺入DNA的效果降低50%。鼻中隔软骨细胞在沉淀培养物中表达低 ALPase 活性。然而，鼻中隔软骨细胞在 10% 血清中以 27 g 离心 23 天，导致 ALPase 活性增加 10 倍。下颌软骨细胞在沉淀培养物中表达高 ALPase 活性。下颌软骨细胞在 10% 血清中以 27 g 离心 7 天，导致 ALPase 活性增加两倍。这些结果表明，生物力学力对鼻中隔和下颌软骨细胞的生长具有不同的影响，并刺激培养物中软骨细胞的分化和钙化。

项目成果

井上 博之: "軟骨細胞および骨芽細胞様細胞に対する遠心負荷の影響" 第2回バイオメカニクスカンファレンス講演論文集、. 55-56 (1991)

Hiroyuki Inoue：“离心载荷对软骨细胞和成骨细胞样细胞的影响”第二届生物力学会议论文集，55-56（1991）

Inoue,H.at al.: "Stimulations of proteoglycan and DNA syntheses in chondrocytes by centrifugation" J.Dent.Res.(1990)

Inoue, H.at al.：“通过离心刺激软骨细胞中的蛋白多糖和 DNA 合成”J.Dent.Res.(1990)

Y.HIRAKI, et.al.: "Differential modulation of growth and phenotypic expression of chondrocytes in sparse and confluent cultures by growth factors" J.Bone Min.Res.5,. 1077-1085 (1990)

Y.HIRAKI 等人：“生长因子对稀疏和汇合培养物中软骨细胞生长和表型表达的差异调节”J.Bone Min.Res.5，。

H. INOUE, J. KATO, M. SAKUDA, M. IWAMOTO, F. SUZUKI, Y. KATO: "Effects of mechanical forces on proliferation of rabbit growth-plate and articular chondrocytes and their extracellular matrix synthesis in pelleted cultures." Fundamentals of Bone Growth edit

H. INOUE、J. KATO、M. SAKUDA、M. IWAMOTO、F. SUZUKI、Y. KATO：“机械力对颗粒培养物中兔生长板和关节软骨细胞增殖及其细胞外基质合成的影响。”

H.INOUE, et, al.: "Stimulation of proteoglycan and DNA syntheses in chondrocytes by centrifugation" J.Dent.Res.69,. 1560-1563 (1990)

H.INOUE 等人：“通过离心刺激软骨细胞中的蛋白聚糖和 DNA 合成”J.Dent.Res.69，。