The new biological estimation for air pollution, indoor pollution and working environment

空气污染、室内污染和工作环境的新生物学估计

基本信息

批准号：
01570304
负责人：
KODAMA Yasushi
金额：
$ 1.41万
依托单位：
University of Occupational and Environmental Healt
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570304/
关键词：
Air Pollution Indoor Pollution EGF Cytochrome P-450 Nitrogen Dioxide AHH Cigarette Smoke PAH イソ酵素 Hela細胞

项目摘要

In this research project, we studied new biological indicators which were useful to assess the risk of chronic obstructive lung diseases and cancer promoting action by air pollution, indoor pollution and working environment. The biological effects of main pollutans, i. e. cigarette smoke, nitorgen dioxide (NO_2), benzo[a]pyrene (BaP), etc, were investigated in vivo and in vitro. Rats were exposed to side stream of cigarette smoke and NO_2 in an experimental chamber, 8 hours/day for 5 days. The activities of aryl hydrocarbon hydroxylase of lung, kidney and liver were increased by the exposure to cigarette smoke, but not by NO_2. Though total contents of cytochrome P-450 were not induced by either cigarette smoke or NO@_2, the dose-dependent inductions of cytochrome P-450b, c, d isozymes by the cigarette smoke wererecognized using Western Immunoblot analysis. The protein concentration, alkaline phosphatase and lactate dehydrogenase acticities in the bronchoalveolar lavage fluid (BALF) were also increased in the rats exposed to cigarette smoke or NO_2. The change of BALF was the most sensitive indicator that we examined in this study. The binding assay of epidermal growth factor (EGF) was also performed using HeLa cell and C3H/1OT1/2 cell. The binding of EGF to EGF receptor reached at a plateau 60-80 min after starting the incubation. This binding was varied by the density of cells on the plate. The plate with low density of the cells shows high binding capacity per cell number. We examined the effect of BaP on the EGF binding in the condition of the same density of the cells, however, it was not recognized that BaP inhibits EGF binding. In this experiment, some biological indicators did not show the result that we had expected. Further investigations were required to clarify the reason.

在这个研究项目中，我们研究了新的生物指标，这些指标有助于评估空气污染、室内污染和工作环境导致的慢性阻塞性肺疾病和癌症促进作用的风险。主要污染物的生物效应，即。 e.对香烟烟雾、二氧化氮(NO_2)、苯并[a]芘(BaP)等进行了体内和体外研究。大鼠在实验室内暴露于侧流香烟烟雾和NO_2中，每天8小时，持续5天。吸烟使肺、肾、肝芳烃羟化酶活性升高，但NO_2不升高。虽然细胞色素P-450的总含量不是由香烟烟雾或NO@_2诱导的，但是使用Western免疫印迹分析识别出香烟烟雾对细胞色素P-450b、c、d同工酶的剂量依赖性诱导。暴露于香烟烟雾或NO_2的大鼠的支气管肺泡灌洗液(BALF)中的蛋白质浓度、碱性磷酸酶和乳酸脱氢酶活性也增加。 BALF 的变化是我们在本研究中检查的最敏感的指标。还使用 HeLa 细胞和 C3H/1OT1/2 细胞进行表皮生长因子 (EGF) 的结合测定。 EGF与EGF受体的结合在开始孵育后60-80分钟达到平台。这种结合因板上细胞的密度而变化。细胞密度低的板显示出每个细胞数的高结合能力。我们在相同细胞密度的条件下检测了BaP对EGF结合的影响，然而，并没有认识到BaP抑制EGF结合。在本次实验中，一些生物指标并没有达到我们预期的结果。需要进一步调查以查明原因。