Establishment of Genomics Based Hepatology

基于基因组学的肝病学建立

基本信息

批准号：
13854015
负责人：
KANEKO Shuichi
金额：
$ 76.54万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (S)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2005
项目状态：
已结题

项目摘要

Results are summarized according to the four objects of this study.1.Construction of comprehensive gene expression profile database of liver diseases.Data base of gene expression profile of liver diseases were constructed with the clinical information, which is the biggest human liver database in the world.2.Analysis of liver diseases.Comprehensive analysis of gene expression of samples from chronic hepatitis B and C, autoimmune hepatitis, primary biliary cirrhosis, hepatocellular carcinoma were performed using serial analysis of gene expression (SAGE) and DNA chips, and demonstrated new findings on the diagnosis and understanding of the pathogenesis of those diseases.3.Development of new methods for the analysis, diagnosis, and treatment of liver diseases.Tissue specific region was found in the human chromosome by using the database #1. This method enables the discovery of tissue specific gene in the chromosomal region. The efficacy of treatment for liver disease was successfully predicted by the comprehensive gene expression analysis.4.Detection of hepatic abnormality, which is not considered as important diseases.Expression profile of diabetic liver with or without obesity was comprehensively analyzed. Gene expression in the diabetic liver was clearly different from that in non-diabetic liver. Furthermore, genes relating with angiogenesis were up-regulating in the liver, indicating that diabetic liver is directly relating with systemic complication including atherosclerosis.

根据本研究的四个目标对结果进行总结：1.肝病综合基因表达谱数据库的构建。根据临床信息构建了肝病基因表达谱数据库，是目前国内最大的人类肝脏数据库。世界.2.肝脏疾病分析。利用基因表达系列分析（SAGE）和DNA芯片对慢性乙型和丙型肝炎、自身免疫性肝炎、原发性胆汁性肝硬化、肝细胞癌样本的基因表达进行综合分析，并证明诊断和了解这些疾病发病机制的新发现。3.开发分析、诊断和治疗肝病的新方法。利用数据库#1在人类染色体中发现了组织特异性区域。该方法能够发现染色体区域中的组织特异性基因。通过全面的基因表达分析，成功预测了肝脏疾病的治疗效果。4.检测不被认为是重要疾病的肝脏异常。对伴有或不伴有肥胖的糖尿病肝脏的表达谱进行综合分析。糖尿病肝脏中的基因表达与非糖尿病肝脏中的基因表达明显不同。此外，肝脏中与血管生成相关的基因上调，表明糖尿病肝脏与包括动脉粥样硬化在内的全身并发症直接相关。