Relation between the mating type of fungus and mycorrhizal symbiosis

真菌交配类型与菌根共生关系

• 批准号: 13660149
• 负责人: UMATA Hidetaka
• 金额: $ 2.11万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660149/
• 关键词: chlorophyll-less orchid; Erythrorchis ochobiensis; Mating type; Mycorrhizal symbiosis; Nuclear phase; Pleurotus ostreatus; Tetrapolarity; シイタケ; 系統間変異; 菌糸生長; ラン型菌根; エリンギ; 菌寄生植物; 木材腐朽

A chlorophyll-less orchid, Erythrorchis ochobiensis, was co-cultured with different isolates of a fungus Pleurotus ostreatus, to clarity the influence of the fungus nuclear phase and mating type on their symbiosis. Four sib-monokaryons and two reconstituted dikaryons obtained from the progeny of a single strain of this tetra-polar fungus were used. These isolates induced seed germination of the orchid whereas germination was not induced without them. One monokaryotic isolate with compatible mating type, A1B1, induced 44% seed germination and moderate growth of the orchid, while the other, A2B2, induced only 6% and very slow growth. Reconstituted dikaryotic isolate, A1B1xA2B2, induced high germination rate, over 60%, with markedly better growth than A1B1. Different mating type monokaryon A1B2 induced remarkable germination, near to 90%, with satisfactory growth, whereas A2B1 induced below 2% germination. Contrary to A1B1xA2B2, another dikaryon A1B2xA2B1 induced markedly slower growth than A1B2. These results suggested that (1) factor A promotes seed germination and growth of the orchid, and factor A1 has higher promoting ability than A2, (2) factor B prohibits the germination and growth, and B1 has much prohibiting ability than B2, or (2) only B1 strongly prohibits the germination and growth, and B2 promotes the ability of A, and (3) A1B1 and A2B2 in dikaryon A1B1xA2B2 act positively and complementary for the germination and growth whereas A2B1 reduce the A1B2 effect in A1B2xA2B1. Two monokaryons of the same mating type showed difference in the germination rate, that is, 44% and 67%, and growth of the orchid, which might result from nucleic difference. Moreover, the two dikaryons with the same nuclei and different cytoplasm showed almost same in the germination but difference in the growth, which might result from cytoplasmic difference.

将无叶绿素兰花 Erythrochis ochobiensis 与不同的平菇真菌分离株共培养，以阐明真菌核相和交配类型对其共生的影响。使用从这种四极真菌的单一菌株的后代获得的四个同胞单核体和两个重建的双核体。这些分离株诱导兰花种子发芽，而没有它们则不会诱导发芽。一种具有相容交配类型的单核分离株 A1B1 诱导 44% 的种子发芽和兰花的中等生长，而另一种 A2B2 仅诱导 6% 且生长非常缓慢。重组双核分离株 A1B1xA2B2 诱导高发芽率，超过 60%，且生长明显优于 A1B1。不同交配类型的单核体A1B2诱导显着的发芽率，接近90%，且生长令人满意，而A2B1诱导的发芽率低于2%。与 A1B1xA2B2 相反，另一个双核体 A1B2xA2B1 诱导的生长速度明显慢于 A1B2。这些结果表明：（1）因子A促进兰花种子的萌发和生长，并且因子A1比A2具有更高的促进能力，（2）因子B抑制兰花种子的萌发和生长，并且B1比B2具有更大的抑制能力，或者（ 2)只有B1强烈抑制A的发芽和生长，B2促进A的能力，(3)双核体A1B1xA2B2中的A1B1和A2B2对A1B1xA2B2起积极和互补的作用。发芽和生长，而 A2B1 减少 A1B2xA2B1 中的 A1B2 效应。相同交配类型的两个单核体在发芽率和兰花生长方面表现出差异，分别为44%和67%，这可能是由于核体差异造成的。而且，具有相同细胞核和不同细胞质的两个双核体在萌发方面几乎相同，但在生长方面存在差异，这可能是由于细胞质的差异所致。