Histaminergic regulation of the neuroendocrine system: An analysis using microdialysis of the hypothalamus of conscious and freely moving rats.

神经内分泌系统的组胺能调节：使用微透析对有意识和自由活动的大鼠的下丘脑进行分析。

基本信息

批准号：
63570128
负责人：
YAMATODANI Atsushi
金额：
$ 1.41万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570128/
关键词：
Histamine Brain dialysis Microdialysis Neuroendocrine system Hypothalamus Hypophysis Adrenocorticohormone Circadian rhythm

项目摘要

Recently, the histaminergic neurons have been identified in mammalian brains by immunocytochemical methods. The cell bodies of the histaminergic neurons are localized in the tuberomammillary nucleus in the posterior hypothalamic region, while the fibers are found in almost all regions of the brain. Especially in the hypothalamic nuclei, the highest density of the histaminergic fibers were found, suggesting the involvement of the central histaminergic system in the regulation of neuroendocrine system, However, detailed neurochemical mechanism of the histaminergic neurotransmission and its physiological roles are still not clear.Among the brain functions, the hypothalamo-hypophyseal neuroendocrine system is the only one whose function can be determined quantitatively by measurement of the plasma levels of the hormones. In this study, we applied the microdialysis technique to determine the dynamics of the neuronal histamine in the hypothalamus, and we tried to correlate the histamine rele … More ase in the hypothalamus and the levels of several hormones in blood to elucidate the physiological roles of histamine in the neuroendocrine system.By improving the dialysis conditions and the assay sensitivity, we could detect histamine release from anterior hypothalamic area. By several lines of neuropharmacological studies, we could also confirm that histamine measured by the microdialysis was released from the nerve terminals. In the freely moving rats, the histamine release was found to show a clear circadian variation, decreasing during the mid-light period, then rapidly increasing and keeping high levels during the dark period. In another experiment, circadian variation of the plasma levels of corticosterone and ACTH was disappeared by chronic administration of the specific inhibitor of histamine synthesis. In conclusion, the release of endogenous neuronal histamine in rat brain was successfully monitored using a new technique of microdialysis, and this technique will be useful to investigate the histaminergic regulation of the neuroendocrine system. Less

最近，通过免疫细胞化学方法在哺乳动物大脑中发现了组胺能神经元，其细胞体位于下丘脑后部的结节乳头核，而纤维几乎遍布大脑的所有区域。下丘脑核团中发现组胺能纤维密度最高，提示中枢组胺能系统参与神经内分泌系统的调节。组胺能神经传递的详细神经化学机制及其生理作用仍不清楚。在脑功能中，下丘脑-垂体神经内分泌系统是唯一可以通过测量血浆激素水平来定量确定其功能的系统。，我们应用微透析技术来确定下丘脑中神经元组胺的动态，并且我们试图将下丘脑中的组胺酶与下丘脑中的组胺相关联。通过改善透析条件和检测灵敏度，我们可以检测下丘脑前区的组胺释放。通过多项神经药理学研究，我们还可以证实：通过微透析测量组胺从神经末梢释放，在自由活动的大鼠中，发现组胺释放表现出明显的昼夜节律变化，在中等光照期间减少，然后。在另一项实验中，长期给予组胺合成的特异性抑制剂可消除血浆皮质酮和 ACTH 水平的昼夜节律变化。结论是，大鼠脑中内源性神经元组胺的释放。使用微透析新技术成功监测了这一情况，该技术将有助于研究神经内分泌系统的组胺能调节。