ANTIBIOTICS CIRCURATION AND BIOLOGICAL ACCUMURATION IN SEMIENCLOSED SEA

半封闭海中的抗生素循环和生物积累

基本信息

批准号：
12680572
负责人：
MUNEKAGE Yukihiro
金额：
$ 2.18万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680572/
关键词：
Antibiotics for fisheries Biological accumulation Antibiotic-resistant bacteria Shrimp rearing in mangrove area Degradation of antibiotics under UV Mangrove development and environment destruction 魚病マングローブ開発と環境破壊高速液体クロマトグラフ(HPLC)懸濁物質

项目摘要

The concentrations of two kinds of antibiotics (ABPC and OTC) in seawater and bottom mud were measured in the fisheries ground. Other four kinds of antibiotics (trimethoprim (TMP), sulfamethoxazole (SMX), norfoxacin (NFXC), oxolinic acid (OXLA)) were extracted from water and bottom mud in Vietnam shrimp rearing ponds developed in the mangrove area and their concentrations were measured. The degradation rate of antibiotics under UV and its absorption and accumulation in plankton were also measured.1) AMP and OTC concentration in water were fluctuated 0.56-1.4ppb and 0.25-4.9ppb, respectively.2) AMP and OTC concentration in bottom mud in fish culture area were fluctuated 15-25ug/1g dry mud and 0.15-0.35ug/1g dry mud, respectively. Accumulated AMP and OTC in 10cm mud depth in Uranouchi Bay were 8ton and 80kg, respectively.3) Antibiotics were degraded under UV. Its concentration C(t) was indicated by exponential function like as C(t)=C_iexp(-α t), where t is time, α is effected by initial … More concentration, C_i. Low concentration OTC is easy to be degraded, but difficult in high concentration of over lOmM, particularly AMP.4) Plankton grew in the 1/2 F medium with OTC, the higher the OTC concentration, the higher the growth rate of plankton and accumulation rate of OTC into plankton cells.5) Concentration of TMP in Vietnam shrimp pond water was 0.3〜1.1ppm in the surface water, 0.3〜2.1ppm in bottom water, Maximum SMX was 1.8〜5.6ppm, maximum NFXC was 1〜4ppm, OXLA was 0.5〜1.8ppm.6) TMP was accumulated at 0.02〜0.13mg, SMX was 0.01〜0.08mg, NFXC Was 0.1〜2.6mg, OXLA was 0.1mg in bottom wet mud of 1g in Vietnam shrimp pond. Antibiotics were terrible highly contained in water and accumulated in bottom mud of shrimp pond.7) Low degradation antibiotics were contained highly in the seawater and accumulated in the bottom mud. Antibiotics stimulated the growth of plankton, and antibiotics were accumulated in plankton. Antibiotic-resistant bacteria appear in these antibiotic contaminated environments, and shrimp diseases such as WSSV (White spot syndrome shrimp virus), YHSV (Yellow head shrimp virus) and TSSV (Taurin syndrome virus) breakout every year. Less

渔场中测定了海水和底泥中两种抗生素（ABPC和OTC）的浓度，其他四种抗生素（甲氧苄啶（TMP）、磺胺甲恶唑（SMX）、诺氟沙星（NFXC）、奥索林酸（OXLA））。从越南红树林地区开发的养虾池的水和底泥中提取抗生素，并测量其浓度，并测量其在紫外线下的降解率及其吸收和积累。还测量了浮游生物中的 AMP 和 OTC 浓度。1) 水中 AMP 和 OTC 浓度分别波动 0.56-1.4ppb 和 0.25-4.9ppb。2) 养鱼区底泥中 AMP 和 OTC 浓度波动 15-25ug/1g 干泥10cm泥浆深度中分别积累了0.15-0.35ug/1g干泥中的AMP和OTC。浦之内湾分别为8吨和80公斤。3）紫外线下抗生素的降解浓度C(t)用指数函数表示，如C(t)=C_iexp(-α t)，其中t为时间，α受到影响。按初始浓度，C_i低浓度OTC容易被降解，但高浓度超过lOmM时很难降解，特别是AMP。4）浮游生物在1/2 F培养基中生长含OTC，OTC浓度越高，浮游生物的生长速度和OTC进入浮游生物细胞的积累速度越高。5）越南虾塘水中TMP浓度为地表水0.3～1.1ppm，水中0.3～2.1ppm。底水中，SMX 最大含量为 1.8～5.6ppm，NFXC 最大含量为 1～4ppm，OXLA 为0.5～1.8ppm。6)越南虾池1g底湿泥中TMP累积量为0.02～0.13mg，SMX为0.01～0.08mg，NFXC为0.1～2.6mg，OXLA为0.1mg。 7) 海水中低降解抗生素含量较高，并在虾池底泥中积累。抗生素刺激了浮游生物的生长，在这些抗生素污染的环境中，浮游生物中出现了抗生素耐药性细菌，引发了WSSV（白斑综合症虾病毒）、YHSV（黄头虾病毒）和TSSV等虾病。（牛磺酸综合症病毒）每年爆发较少。