Rheological properties in fatty acid salt-induced p-lactoglobulin gel and the changes of secondary structure during the gelation

脂肪酸盐诱导的β-乳球蛋白凝胶的流变特性及凝胶过程中二级结构的变化

• 批准号: 12680152
• 负责人: OHTA Naoko
• 金额: $ 1.98万
• 依托单位: Junior College at Mishinia, Ninon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680152/
• 关键词: β-lactoglobulin; gel; fatty acid salt; dynamic viscoelasticity; Fourier transform infrared spectroscopy; protein secondary structure; レオロジー測定; FTIR; クライオ-TEM

In this study, we found that transparent gels of β-lactoglobulin (P-LG) were formed only by the addition of fatty acid salts (FAS), such as sodium caprate, to the protein solutions at ambient temperature. We investigated the Theological properties of the FAS-induced gel by the analyses of dynamic viscoelasticity. Furthermore we analysed the changes in the secondary structure of proteins during the gelation step by Fourier transform infrared (FT-IR) spectroscopy. In β-LG protein in which the original contents are predominant β-sheet, intermolecular β-sheet bands increased with the decrease in the content of intramolecular β-sheet. FAS-induced gel was heated at 80℃ for 30 min after the gel was formed, and much increment of the intermolecular β-sheet bands by heat treatment was observed. These results suggest that the formation of FAS-induced gels of β-LG were accompanied by less marked changes in the protein conformation than those in heat-induced gels.

在这项研究中，我们发现只有在环境温度下向蛋白质溶液中添加脂肪酸盐 (FAS)（例如癸酸钠）才能形成透明的 β-乳球蛋白 (P-LG) 凝胶，我们研究了其流变特性。此外，我们通过傅里叶变换红外（FT-IR）光谱分析了凝胶步骤中蛋白质二级结构的变化。原始含量以β-折叠为主的蛋白质，FAS诱导凝胶成胶后，80℃加热30 min，分子间β-折叠条带增多，分子内β-折叠条带增多。观察到热处理引起的分子间β-折叠带的增加，这些结果表明FAS诱导的β-LG凝胶的形成伴随着比热诱导的凝胶更不显着的蛋白质构象变化。