Investigation of c-fos and preprodynorphin induced by noxicious stimulation on tooth

牙齿毒性刺激诱导的c-fos和前强啡肽的研究

• 批准号: 12671922
• 负责人: IGARI Toshirou
• 金额: $ 2.24万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671922/
• 关键词: c-fos; noxiciou stimulant; tooth pain; preprodynorphin

Most uncuarable pain in oral lesion occurred after chronic pulpitis or periodopntitis which present the symptom like the deafferention pain. So we start the research for c-fos, one of the cancer related gene, is known to present in the dorsal horn of the spinal cord and the special area of brain, as a new marker of those oral pain. Under light halothane anesthesia reveal a rat's upper jaw nerve (1)cut the upper jaw nerve (Model I), (2)Chronic constriction injury (CCI) model (Model II) (3)sealed with paraformaldehyde in rat's cisal tooth pulp, chronic pulpal tooth model (Model III)Samples were taken each 30minite to 3hours, and each one week to 3 months.After noxious stimulation, the numbers of the c-fos positive cell on the dorsal horn of C1 and C2 cervical cord, and trigeminal nucleus caudalis (TNC) were presented in the stimulated group. But it also revealed in the sham group. (p<0.001 ; between the all group at the same area).These results don't indicate that the noxious stimulus induces the gene expression of c-fos on the dorsal horn and TNC.The c-fos, therefore, is not useful biochemical index for the evaluation of pain expression on nerve level in our research.

口腔病变中最无关的疼痛发生在慢性肺炎或周期性炎后炎后表现出症状，例如脱附疼痛。因此，我们开始研究C-Fos是癌症与癌症相关的基因之一，已知存在于脊髓的背角和大脑的特殊区域，作为这些口腔疼痛的新标志。在轻烟硫烷麻醉下，大鼠的上颌神经（1）切断了上颌神经（I），（2）长期收缩损伤（CCI）模型（CCI）模型（II）（3）用大鼠的cisal牙齿浆中的多甲醛密封，慢性牙髓牙齿模型（III III型）的刺激量，每个30mities to 30mities to to to to to 30 minity samples，在刺激组中介绍了C1和C2宫颈索的背角以及三叉神经核（TNC）的C-FOS阳性细胞的数量。但这也在假小组中揭示了。 （p <0.001；在同一区域的所有组之间）。这些结果并未表明有害刺激在背角和TNC上诱导c-fos的基因表达。因此，C-FOS并非有用的生化指标来评估我们研究中神经上疼痛表达的疼痛表达。