Research on Apoptosis after Hemorrhagic Shock -Hypertonic Saline Resuscitation-

失血性休克后细胞凋亡的研究-高渗盐水复苏-

基本信息

批准号：
12671498
负责人：
MURAO Yoshinori
金额：
$ 1.98万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671498/
关键词：
Hemorrhagic Shock Hypertonic Saline Apoptosis TUNEL DNA ladder caspase-3 DNA ladder Caspase-3

项目摘要

Male Sprague-Dawley rats were hemorrhaged until a mean arterial pressure (MAP) of 40 +/- 5 mmHg was reached. This level of hypotension was sustained for 1 h, and the animals were randomly assigned to the following groups by resuscitation methods. Control, Sham, HS + SB : (4 mg/Kg of 7.5 % NaCl solution: HS) and shed blood (SB) at the same time, 2LR + SB : hemorrhage shock for 1 h and resuscitated with Lactated Ringer's solution (2 times the volume of the shed blood) + SB, 3LR (3 times the volume of the shed blood). Lungs were harvested and total histology scores were examined at 48 h after hemorrhage and resuscitation. HS + SB group significantly prevented lung damage compared with other groups (p < O.05). The effect of hypertonic saline resuscitation on the time course of intestinal damage and apoptosis after hemorrhagic shock in mice was invesigated. After anesthesia, male BALB/c mice were hemorrhaged to the mean arterial pressure of 40 +/- 5 mmHg for 90 min.Resuscitation was made by … More HS + SB or 2LR + SB. Intestinal injury was devised and based on the extent of the vacuolation at basal area of the intestinal villi. Apoptosis score of the small intestines was examined by TUNEL method and DNA ladder assay. Activation of caspase-3 expression was assessed, by Western blot. Hemorrhagic shock and resuscitation induced the apoptosis in 2LR + SB at 2 h and intestinal damage in 2LR + SB at 6h compared to other groups significantly (p < 0.01) Apoptosis index of HS + SB at 2h group was significantly reduced compared to 2LR+SB at 2h (p < 0.01) and also intestinal damage of HS + SB at 6h group was significantly reduced compared to 2LR + SB at 6h (p < 0.01).Activation of caspase-3 of HS + SB at 2h group was reduced compared to 2LR + SB at 2h group. Conclusions: Hypertonic saline resuscitation reduced the apoptosis and intestinal damage after hemorrhagic shock and resuscitation. Thus, the effect of hypertonic saline resuscitation toward apoptosis may have important role in hemorrhagic shock and resuscitation. Less

雄性Sprague-Dawley大鼠失血直至平均动脉压(MAP)达到40+/-5mmHg，该低血压水平持续1小时，并且通过复苏对照方法将动物随机分配到以下组。 , Sham, HS + SB : (4 mg/Kg 7.5 % NaCl 溶液: HS) 并同时流血 (SB), 2LR + SB :失血性休克1小时，用乳酸林格氏液（2倍流血量）+SB、3LR（3倍流血量）复苏。出血后48小时采集肺并检查总组织学评分。与其他组相比，HS + SB 组显着预防了肺损伤（p < O.05）。研究了小鼠失血性休克后肠道损伤和细胞凋亡的情况。麻醉后，将雄性 BALB/c 小鼠失血至平均动脉压 40 +/- 5 mmHg，持续 90 分钟。采用 HS + SB 或 2LR 进行复苏。 + SB.根据小肠绒毛基底区域的空泡程度设计肠损伤。与其他组相比，通过TUNEL方法检查肠道并通过Western blot评估失血性休克和复苏诱导2LR + SB中的细胞凋亡和2LR + SB中6小时的肠道损伤。各组 (p < 0.01) 2h 时 HS + SB 组细胞凋亡指数较 2LR+SB 2h 组显着降低 (p < 0.01)，并且与 6h 时的 2LR + SB 相比，6h 组的 HS + SB 的肠道损伤显着减少 (p < 0.01)。与 2LR + SB 时的 2h 组相比，2h 组的 HS + SB 的 caspase-3 激活减少结论：高渗盐水复苏可减轻失血性休克复苏后的细胞凋亡和肠道损伤。高渗盐水复苏对细胞凋亡可能在失血性休克和复苏中发挥重要作用。