Research on Apoptosis after Hemorrhagic Shock -Hypertonic Saline Resuscitation-

失血性休克后细胞凋亡的研究-高渗盐水复苏-

基本信息

批准号：
12671498
负责人：
MURAO Yoshinori
金额：
$ 1.98万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671498/
关键词：
Hemorrhagic Shock Hypertonic Saline Apoptosis TUNEL DNA ladder caspase-3 DNA ladder Caspase-3

项目摘要

Male Sprague-Dawley rats were hemorrhaged until a mean arterial pressure (MAP) of 40 +/- 5 mmHg was reached. This level of hypotension was sustained for 1 h, and the animals were randomly assigned to the following groups by resuscitation methods. Control, Sham, HS + SB : (4 mg/Kg of 7.5 % NaCl solution: HS) and shed blood (SB) at the same time, 2LR + SB : hemorrhage shock for 1 h and resuscitated with Lactated Ringer's solution (2 times the volume of the shed blood) + SB, 3LR (3 times the volume of the shed blood). Lungs were harvested and total histology scores were examined at 48 h after hemorrhage and resuscitation. HS + SB group significantly prevented lung damage compared with other groups (p < O.05). The effect of hypertonic saline resuscitation on the time course of intestinal damage and apoptosis after hemorrhagic shock in mice was invesigated. After anesthesia, male BALB/c mice were hemorrhaged to the mean arterial pressure of 40 +/- 5 mmHg for 90 min.Resuscitation was made by … More HS + SB or 2LR + SB. Intestinal injury was devised and based on the extent of the vacuolation at basal area of the intestinal villi. Apoptosis score of the small intestines was examined by TUNEL method and DNA ladder assay. Activation of caspase-3 expression was assessed, by Western blot. Hemorrhagic shock and resuscitation induced the apoptosis in 2LR + SB at 2 h and intestinal damage in 2LR + SB at 6h compared to other groups significantly (p < 0.01) Apoptosis index of HS + SB at 2h group was significantly reduced compared to 2LR+SB at 2h (p < 0.01) and also intestinal damage of HS + SB at 6h group was significantly reduced compared to 2LR + SB at 6h (p < 0.01).Activation of caspase-3 of HS + SB at 2h group was reduced compared to 2LR + SB at 2h group. Conclusions: Hypertonic saline resuscitation reduced the apoptosis and intestinal damage after hemorrhagic shock and resuscitation. Thus, the effect of hypertonic saline resuscitation toward apoptosis may have important role in hemorrhagic shock and resuscitation. Less

将雄性Sprague-Dawley大鼠大鼠出血，直到达到40 +/- 5 mmHg的平均动脉压（MAP）。这种低血压持续了1小时，并通过复苏方法将动物随机分配给以下组。对照，假，HS + SB ：（ 4 mg/kg的NaCl溶液：HS）和Shed Blood（SB）同时，2LR + SB：出血性休克1 h，用乳酸铃声的溶液复苏（降低血液的体积的2倍） + SB，3LR，3LR，3LR（3倍的体积）。收集肺部，并在出血和复苏后48小时检查总体组织学评分。与其他组相比，HS + SB组显着防止了肺损伤（P <O.05）。研究了小鼠出血性休克后高渗盐水复苏对肠道损伤和凋亡的时间过程的影响。麻醉后，将雄性BALB/C小鼠出血至40 +/- 5 mmHg的平均动脉压90分钟。设计了肠道损伤，并根据肠绒毛基本区域的真空化程度。通过TUNEL方法和DNA阶梯测定法检查了小肠的凋亡评分。通过蛋白质印迹评估了caspase-3表达的激活。与其他组相比，与其他组相比，2 h + Sb的2 h + sb的2 hr + Sb的凋亡诱导了2HR + Sb的肠道凋亡（p <0.01）2H组的HS + Sb的凋亡指数显着降低了6H（p <0.01）。与2H组的2LR + SB相比。结论：高渗盐水复苏减少了出血性休克和复苏后的凋亡和肠道损伤。这就是高渗盐酸复苏对凋亡的影响可能在出血性休克和复苏中起重要作用。较少的