BMP Signaling on Cartilage Repair Response in Full-thickness Articular Cartilage Defects

BMP 信号对全层关节软骨缺损软骨修复反应的影响

基本信息

批准号：
12671425
负责人：
NAKAMURA Eiichi
金额：
$ 1.98万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671425/
关键词：
Articular Cartilage Bone Morphogenetic Protein Chondrocyte 骨形戒因子

项目摘要

We investigated BMP signaling on cartilage repair response in full-thickness articular cartilage defects. In the previous study, we established a rat model to clarify cartilage repair response full-thickness articular cartilage defects. The repair process in the center of the defects was analyzed by immunostaining and in situ hybridyzation method at 2,4,7,14 days after surgery using our rat model with a small defect (0.7mm width) and a large defect (1.5mm width). In the center of a small defect, at 2 days after surgery, the messenger RNA of BMP-4 was found, but this protein was not observed. After that, the messenger RNA and protein of BMP-4 was found through all periods. The messenger RNA and protein of BMP-6 was first found at 7 days after surgery. The messenger RNA and protein of GDF-5 was slightly first found at 4 days after surgery. The messenger RNA and protein of those receptors was expressed through all periods. On the other hand, in the center of a large defect, at 2 days after surgery, the messenger RNA of BMP-4 was found without no protein. At 4days after surgery, the messenger RNA and protein of BMP-4 was slightly expressed. At 7 days after surgery, the messenger RNA of BMP-4 was only observed. At 7 days after surgery, the messenger RNA and protein of BMP-4 were not found. The messenger RNA and protein of BMP-6 and GDF-5 was not observed through all periods. The messenger RNA and protein of those receptors was found through all periods as well as those in a small defects. Based on the results of this study, it was certified that, on cartilaginous repair in full-thickness articular cartilage defects, BMP-4 signaling is important for the initiation of the process, BMP-6 signaling may be involved in chondrocyte maturation and hypertrophy, GDF-5 signaling may be involved in chondrocyte hypertrophy

我们研究了全厚度关节软骨缺陷的软骨修复响应的BMP信号传导。在先前的研究中，我们建立了一个大鼠模型，以阐明软骨修复响应全厚度关节软骨缺陷。使用具有较小缺陷（0.7mm宽度）和较大的缺陷（1.5mm宽度），通过免疫染色和原位杂化方法分析了缺陷中心的修复过程。）。在小缺陷的中心，在手术后2天，发现了BMP-4的信使RNA，但没有观察到该蛋白质。之后，在所有时期都发现了BMP-4的信使RNA和蛋白质。 BMP-6的信使RNA和蛋白质是在手术后7天发现的。 GDF-5的信使RNA和蛋白质在手术后4天略微发现。这些受体的信使RNA和蛋白质在所有时期都表达。另一方面，在大缺陷的中心，在手术后2天，发现BMP-4的信使RNA没有蛋白质。手术后的4天，略微表达了BMP-4的信使RNA和蛋白质。手术后7天，仅观察到BMP-4的信使RNA。手术后7天，找不到BMP-4的信使RNA和蛋白质。在所有时期都没有观察到BMP-6和GDF-5的信使RNA和蛋白质。这些受体的信使RNA和蛋白质均在各个时期以及小缺陷中发现。根据这项研究的结果，证明，在全厚度关节软骨缺陷的软骨修复中，BMP-4信号对于启动该过程很重要，BMP-6信号可能与软骨细胞的成熟和肥大有关GDF-5信号传导可能与软骨细胞肥大有关