The potential storage of peripheral nerve grafts using polyphenol

使用多酚储存周围神经移植物的潜力

• 批准号: 12671412
• 负责人: KAKINOKI Ryosuke
• 金额: $ 2.18万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671412/
• 关键词: Polyphenol; storage of peripheral nerve grafts; peripheral nerve regeneration; 神経保存; 末梢神経再生; Polyphenol; storage of peripheral nerve grafts; peripheral nerve regeneration; 神経保存; 末梢神経再生

The potential storage of peripheral nerve grafts using polyphenol was assessed in a rat sciatic nerve model. Isogenic sciatic nerve grafts 20 mm in length were stored either in polyphenol or Dulbecco's Modified Eagle Medium (DMEM) prior to transplantation. Isografting of fresh nerves was also done. Graft cells were kept more viable in the polyphenol-treated nerve segments than in the nerves stored in culture medium. The histological and electrophysiological outcomes of nerve regeneration through polyphenol-treated nerves was significantly better than those through nerves stored in DME medium and almost equal to those through fresh nerve grafts at 24 weeks postengraftment,. This study shows the potential ability of tissue preservation using polyphenol.

在大鼠坐骨神经模型中评估了使用多酚的周围神经移植物的潜在存储。移植前，将等源性坐骨神经移植长度为20 mm，或者存储在多酚或Dulbecco改良的Eagle培养基（DMEM）中。还完成了新鲜神经的等法。与培养基中存储的神经相比，在多酚处理的神经段中保持移植细胞更加可行。通过多酚治疗的神经的神经再生的组织学和电生理结局明显优于通过培养基中DME培养基中存储的神经，几乎等于通过新鲜神经移植物在移植后通过新鲜神经移植的神经。这项研究显示了使用多酚的组织保存的潜在能力。