Molecular indentification of the pathway for amino acids relased from swollen cell

肿胀细胞释放氨基酸途径的分子鉴定

• 批准号: 12670051
• 负责人: HAZAMA Akihiro
• 金额: $ 2.11万
• 依托单位: Okazaki National Research Institutes,Center for Integrative Bioscience
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670051/
• 关键词: glutamic acid; cell swelling; hyototonic; ischemia; C6; glia; channek; chioride; ATP

Glutamic acid released from glial cells has pathophysiological roles in brain under ischemic conditions. The pathways for the glutamic acid release are still unknown. We investigate the characteristics of the glutamic acid release from the hyposmotically swollen glioma cell line (C6). First we established the efficient method for the measurement of glutamic acid using glutamic acid dehydorgenease and the chromogenic substrate for NAD. With this method, we measured the glutamic acid released from C6 cells in the hypotonic solutions. The extracellular concentration of glutamic acid increased from 〜3uM under the isotonic condition to 〜10 uM under the hypotonic condition. The glutamic acid release was less sensitive to the temperature between 16℃ and 36℃, suggesting that glutamic acid might be released via the channel pathway, not via transporters nor exocytosis. Since volume-sensitive Cl channels are candidates for the pathway for glutamic acid release, we tested several reagents, including phloretin and arachidonic acid, reported to block volume-sensitive Cl channels and found that those failed to inhibit glutamic acid release from C6 cells. Gd, the blocker for ATP release from swollen cells, also failed to block glutamic acid release. Taken together, we conclude that glutamic acid is released from C6 cells under hypotonic condition via the channel pathway, which is distinct from Cl channels or the permeation pathway for ATP.

来自缺血条件下的谷氨酸在大脑中具有病理生理学作用Methos。由于体积敏感的Cl通道是谷氨酸释放的途径，我们可以通过转移途径而不是通过转运蛋白或胞吐作用释放。 C6细胞GD是ATP从螺旋细胞释放的阻滞剂，也无法通过通道途径阻断谷氨酸释放。

项目成果

挾間章博: "Glutamic acid release from the swollen glioma cell line"Cell Structure and Function. 26. 405-405 (2001)

Akihiro Hasama：“肿胀的神经胶质瘤细胞系释放谷氨酸”细胞结构和功能。26. 405-405 (2001)。

Akihiro Hazama: "GIutamic Acid Release from the Swollen Glioma Cell Line"Cell Structure and Function,. 26. 405 (2001)

Akihiro Hazama：“肿胀的神经胶质瘤细胞系释放谷氨酸”细胞结构和功能。