Study on the inherited chondrodysplasia of Japanese brown catties

日本褐猫遗传性软骨发育不良的研究

基本信息

批准号：
12660292
负责人：
YAMANO Shuji
金额：
$ 2.11万
依托单位：
Nippon Veterinary and Animal Science University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Inherited chondrodysplasia has been reported in Japanese brown cattle. This study investigated the differentiation and proliferation of chondrocytes in the epiphyseal growth plates (EGPs) of the long bones in the limbs of cattle with this chondrodysplasia. The proximal tibiae, distal femur, and proximal humerus, in three cases of chondrodyspiasia, were examined by immunohistochemistry for type X collagen, type II collagen, and 5-bromo-2'-deoxyuridine (BrdU). Type X collagen immunoreactivity was detected in hypertrophic chondrocytes in the calcified cartilage matrix deep within the central region of the EGPs. Bone tissue and small vessels occasionally invaded the calcified cartilage matrix. In the lateral region, type X collagen immunoreactivity was detected in the cartilage matrix of the reduced hypertrophic zone on the metaphyseal side. Type II collagen immunoreactivity was observed in the cartilage matrix throughout the EGPs, in all cases, to a degree similar to that detected in normal cattle. A few chondrocytes incorporating BrdU were detected in the lateral region, where a columnar arrangement of chondrocytes was maintained. These findings indicate that irregular differentiation into hypertrophic chondrocytes occurs within the EGPs in the chondrodysplasia found in Japanese brown cattle. The calcified cartilage matrix synthesized by these hypertrophic chondrocytes is removed and replaced by bone tissue, and ultimately the EGP disappears in places. The deformed appearance of the epiphyses of the long bones appears to be caused by an imbalance in proliferation and differentiation in the central and lateral regions of EGPs.

据报道，日本棕牛有遗传性软骨增生。这项研究调查了通过这种软骨发育不全的牛四肢的长骨的外周期生长板（EGP）中软骨细胞的分化和增殖。在三种软骨症的情况下，通过免疫组织化学检查了近端胫骨，股骨远端和近端肱骨，用于X型胶原蛋白，II型胶原蛋白和5-Bromo-2'-脱氧甲酰胺（BRDU）的免疫组织化学。 X型胶原蛋白免疫反应在EGP的中央区域深处的钙化软骨基质中检测到。骨组织和小血管偶尔会入侵钙化的软骨基质。在外侧区域，在形而上学侧的肥厚区的软骨基质中检测到X型胶原蛋白免疫反应性。在所有情况下，在整个EGP的软骨基质中都观察到II型胶原蛋白免疫反应性，在所有情况下，与正常牛中检测到的有关。在横向区域中检测到一些结合BRDU的软骨细胞，其中保持软骨细胞的柱状排列。这些发现表明，在日本棕牛中发现的软骨发育不良中的EGP内发生了不规则分化为肥厚的软骨细胞。这些肥厚的软骨细胞合成的钙化软骨基质被去除并用骨组织代替，最终在某些地方消失了EGP。长骨的外周期外观的变形似乎是由于EGP的中央和侧向区域的增殖和分化不平衡引起的。