Efficacy of Freeze-thawed Peripheral Nerve Allograft in the Dog

冻融狗同种异体周围神经移植物的疗效

• 批准号: 12833002
• 负责人: TOMIZAWA Nobuyuki
• 金额: $ 2.18万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12833002/
• 关键词: freeze-thawed nerve allograft; peripheral nerve; Schwann cell; dog; rat; 同種冷凍神経移植

To obtain the fundamental information firstly, the functional recovery from peripheral nerve defects was studied by carrying out freeze-thawed nerve allograft and autograft of rat sciatic nerves. Although several neurological examinations were performed after nerve grafting, only superficial pain perception was recovered within the 110 days postoperatively in most animals. Sciatic function index was not effective to evaluate the motor nerve function because of individual deviations.To investigate the efficacy of Schwann cell (SC) transplantation with freeze-thawed peripheral nerve allograft, 4 types of SC cultures from adult rat sciatic nerves were compared. The method by Calderon-Martinez was the most excellent technique on viability and purity of SCs. The same method for culturing SCs was performed in adult beagle dogs. As a result, the purity of SCs was about 90% and the number of SCs was about 3.6×10^7 from 3cm of dog sciatic nerve.In order to evaluate the application of SC cultures along with freeze-thawed nerve allograft, co-culture of SCs and freeze-thawed nerve grafts, and the direct injection of cultured SCs to freeze-thawed nerve grafts were performed. No living SCs were observed in co-cultured nerve graft. On the other hand, there were many viable SCs in nerve graft by the direct injection of cultured SCs to freeze-thawed nerve grafts.From the results of this study, it was elucidated that freeze-thawed nerve allograft was effective on the recovery of sensory function, and SC culture from adult rat sciatic nerves was available for promotion of nerve regeneration. Moreover, it was proved that SC culture from adult dogs was also possible, and the direct injection of cultured SCs to nerve grafts was effective for the application of SC cultures along with freeze-thawed nerve allograft.

为了获得基本信息，首先，通过执行冻融性神经同种异体移植和大鼠坐骨神经自体移植，从周围神经缺陷中恢复了功能性恢复。尽管神经交接后进行了几次神经检查检查，但由于大多数动物，在110天内仅回收了浅表疼痛感知。坐骨神经功能指数无法有效地评估运动神经功能，这是由于单个出发的效果。要研究用冻干的外周神经同种异体移植的Schwann细胞（SC）移植的效率，比较了成人大鼠坐骨神经的4种SC培养物。 Calderon-Martinez的方法是SCS的可行性和纯度最出色的技术。在成人小犬犬中也采用了类似SC的聚类SC。 As a result, the purity of SCs was about 90% and the number of SCs was about 3.6×10^7 from 3cm of dog sciatic nerve.In order to evaluate the application of SC cultures along with freeze-thawed nerve allograft, co-culture of SCs and freeze-thawed nerve grafts, and the direct injection of cultured SCs to freeze-thawed nerve grafts were performed. No living SCs were observed in co-cultured nerve grafts.On the other hand, there were many viable SCs in nerve graft by the direct injection of cultured SCs to freeze-thawed nerve grafts.From the results of this study, it was elucidated that freeze-thawed nervous allograft was effective on the recovery of sensory function, and SC culture from adult rat sciatic nerves was available for promotion of nerve regeneration.此外，事实证明，成年犬的SC培养物也是可能的，并且直接注射培养的SC向神经移植物可以有效地应用SC培养物以及冻融性的神经同种异体移植物。