Decomposition of low degradable chelating agent by marine bacteria bioremediation of marine environment

海洋细菌分解低降解螯合剂海洋环境生物修复

• 批准号: 12660166
• 负责人: IMADA Chiaki
• 金额: $ 2.24万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660166/
• 关键词: EDTA; Chelating agent; marine bacteria; Pseudoalteromonas; Vibrio; Pseudomonas; Alteromonas; Flavobacterium; Acinetobacter

Chelating agent such as ethylenediaminetetraacetic acid (EDTA)-decomposing bacteria were first isolated from marine environment and characterized. The rate of degradation of EDTA was measured by ion chromatography as ferric chelate. Out of 885 isolates from various sea areas, 8 strains isolated from various sea areas were found to decompose it. Among them, strain H6-2-4 had the highest ability to decompose it. The rate of degradation was approximately 30 % in seawater medium containing 1000 ppm of Fe-EDTA. The experiment using the intact cells revealed that the strain also had an ability to decompose various metal-EDTA chelates such as Ca, Cu and Mg. EDTA was converted into iminodiacetic acid, which was further cleaved into glycine in the second pathway. The strain was a Gram-negative motile rod with polar flagella and required seawater for its growth, which indicated that it was marine in origin. The strain was identified as Pseudoalteromonas atlantica based on the DNA-DNA hybridization with the type culture.

首先从海洋环境中分离出螯合剂，例如乙二胺二乙酸（EDTA） - 分泌细菌。 EDTA降解速率通过离子色谱法作为螯合物测量。在来自各个海洋地区的885个分离株中，发现从各个海区分离的8种菌株将其分解。其中，H6-2-4菌株分解它的能力最高。在含有1000 ppm的Fe-Edta的海水培养基中，降解速率约为30％。使用完整细胞的实验表明，该菌株还具有分解各种金属-EDTA螯合物（例如Ca，Cu和Mg）的能力。 EDTA被转化为二氨酸乙酸，在第二个途径中进一步裂解成甘氨酸。该菌株是带有极性鞭毛的革兰氏阴性运动杆，需要海水生长，这表明它是海洋的起源。基于与类型培养物的DNA-DNA杂交，该菌株被鉴定为假偏蛋白瘤atlantica。