CONSTRUCTION OF A MONITERING SYSTEM OF ENDOCRINE DISRUPTING SUBSTANCES USING MEDAKA

利用青鳉构建内分泌干扰物监测系统

基本信息

批准号：
11839006
负责人：
HAMAGUCHI Satoshi
金额：
$ 2.37万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

The present project was performed in order to develop a reliable system for testing the effects of environmental endocrine disruptors using medaka. Oryzias latipes. We could reaffirm the validity of the medaka as a test organism for the presence of estrogenic substances, not only as a laboratory animal but also as a wild life. The following results were obtained.1. Using a genetic marker on Y chromosome, the genetic sex of a given individual become able to be distinguished exactly. From the screening of wild medaka by this marker, we identified 3 mutations as to the sex differentiation. The sex reversal induced by endocrine-disruptors can also be identified.2. Annual change of vitellogenin content in the serum of wild medaka was clarified. In females, vitellogenin was high in spring and summer, and low in autumn and winter. In males, we could not detected vitellogenin throughout the year. These results indicate that the vitellogenin content of wild medaka is a good indicator of the contamination of estrogenic substances to environment.3. Using two different indicators, the rate of sex reversal and the vitellogenin content, the sensitivity to estrogens of inbred medaka strains was evaluated. The rates of the sex-reversal induced by sex steroids varied among strains. The use of congenic strains as well as recombinant strains clarified that the difference in the rate of sex-reversal can be attributed to a genetic factor that is strongly linked to the sex-determining gene on Y chromosome. We also examined the dose-response relationship and a time-course change of the vitellogenin content in the serum of fish treated with estradiol. Vitellogenin appeared in male fish within 7 days of treatment and a definite dose-dependent relationship was identified. There was no obvious difference in the content of vitellogenin among strains used.

目前的项目是为了开发可靠的系统，用于使用MEDAKA测试环境内分泌干扰物的影响。 oryzias latipes。我们可以重申Medaka作为测试生物的有效性，不仅是实验室动物，而且还作为野生生活。获得以下结果1。在Y染色体上使用遗传标记，给定个体的遗传性别可以准确区分。从该标记对野生Medaka进行筛查，我们确定了3个有关性别差异的突变。也可以识别由内分泌干扰物引起的性逆转。2。阐明了野生Medaka血清中卵黄蛋白含量的年度变化。在女性中，春季和夏季的植物蛋白很高，秋季和冬季低。在男性中，我们全年无法检测到卵黄蛋白。这些结果表明，野生Medaka的植物素含量是雌激素物质对环境污染的良好指标。3。使用两个不同的指标，性别逆转和卵黄蛋白含量的速率，评估了对近交甲状腺菌株雌激素的敏感性。性类固醇引起的性转换率在菌株之间各不相同。先天性菌株的使用以及重组菌株澄清说，性反转率的差异可以归因于与Y染色体上的性别确定基因密切相关的遗传因子。我们还检查了用雌二醇治疗的鱼类血清中植物素含量的剂量反应关系和时间课程的变化。植物素在治疗后的7天内出现在雄性鱼类中，并确定了明确的剂量依赖性关系。所用菌株中卵黄蛋白的含量没有明显的差异。