Correlation between bovine viral diarrhea virus E1 gene and host genome

牛病毒性腹泻病毒E1基因与宿主基因组的相关性

• 批准号: 11836002
• 负责人: YAMATO Osamu
• 金额: $ 2.11万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11836002/
• 关键词: Bovine viral diarrhea mucosal disease; BVD; BVDV; Virus antigen; virus gene; BVD-MD; gp25; E1

Persistently infected cattle with bovine viral diarrhea virus (BVDV) excrete the virus continuously without any clinical signs, and are serious sources of the infection of BVDV in a herd. In order to diagnose the BVDV infection accurately and rapidly, polymerase chain reaction (PCR) has been utilized. By the use of specific primers for detecting p14 and E1 coding regions of BVDV gene, it was possible to discriminate the viral sero types based on the PCR-amplification patterns. However, PCR-amplification patterns of several field isolates from Hokkaido did not correspond to any Japanese BVDV strains. In this study, to clarify the relation between PCR-amplification patterns and viral sero types, a new primer coding the gp25 region of BVDV gene was designed and PCR was performed on Japanese BVDV strains, field isolates, and bovine leucocytes. Then nucleotide sequences and deduced amino acid sequences of the PCR products were compared. Nucleotide sequences and deduced amino acid sequences of the PCR products were highly homologous each other. Therefore E1 seemed to be not concerned in different PCR-amplificatiion pattern. The analysis of amino acid sequences indicated that the hydrophobicity of E1 was highly conserved among each PCR products examined in this study. This region might be a transmembrane domain of E1, and have a function as an anchor for E2 and E0 which induces neutralizing antibodies.Additionally, highly homologous gene with E1 of BVDV was detected from bovine genome in leucocytes. This gene was recognized in not only BVDV infected cattle but also in uninfected cattle. The mRNAs of this gene were also detected from all cattle examined. This gene might play an important role in attachment or invasion of BVDV to host cell.

用牛病毒腹泻病毒（BVDV）持续感染的牛不断地排出病毒，而没有任何临床迹象，并且是BVDV感染的严重来源。为了准确，快速诊断BVDV感染，已经使用了聚合酶链反应（PCR）。通过使用特定引物检测BVDV基因的p14和E1编码区，可以根据PCR扩增模式区分病毒血清类型。但是，来自北海道的几种场分离株的PCR扩增模式与任何日本BVDV菌株都不相对应。在这项研究中，为了阐明PCR扩大模式与病毒血清类型之间的关系，设计了编码BVDV基因的GP25区域的新启动器，并对日本的BVDV菌株，田间分离株和牛白细胞进行了PCR。然后比较了PCR产物的核苷酸序列和推导的氨基酸序列。 PCR产物的核苷酸序列和推导的氨基酸序列相互高度同源。因此，E1似乎并不关心不同的PCR-Amplificatiion模式。对氨基酸序列的分析表明，在本研究中检查的每种PCR产物中，E1的疏水性是高度保守的。该区域可能是E1的跨膜结构域，并且具有诱导中和抗体的E2和E0的锚点。从白细胞中的牛基因组中检测到具有BVDV的E1高度同源基因。该基因不仅在受BVDV感染的牛，而且在未感染的牛中得到认可。还从检查的所有牛中检测到了该基因的mRNA。该基因可能在BVDV对宿主细胞的附着或侵袭中起重要作用。

项目成果

M.Tajima: "Prevalence of genotypes of bovine viral diarrhea viruses in Lower Saxony, Germany"Virus Res.. (in press). (2001)

M.Tajima：“德国下萨克森州牛病毒性腹泻病毒基因型的流行”病毒研究（正在出版）。

M.Tajima: "Possible causes of diabetes mellitus in cattle infected with bovine viral diarrhoea virus"J.Vet.Med.B. 46. 207-215 (1999)

M.Tajima：“感染牛病毒性腹泻病毒的牛患糖尿病的可能原因”J.Vet.Med.B。

M.Tajima: "Possible causes of diabetes mellitus in cattle infected with bovine viral diarrhoea virus"J.Vet.Med.. B46. 207-215 (1999)

M.Tajima：“感染牛病毒性腹泻病毒的牛患糖尿病的可能原因”J.Vet.Med.. B46。