Research system on effective markers for analyzing genetic variances in allied taxa.

分析近缘类群遗传变异的有效标记研究体系。

• 批准号: 11833009
• 负责人: KOSUGE Keiko
• 金额: $ 1.92万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11833009/
• 关键词: RAPD maker; Myrica rubra; Brachyscome; seed dispersal; wind pollination; retrotransposon; genome size; ゲノム再構成; 転位因子; 優性マーカー; 共優性マーカー; 花粉散布; 近交弱勢

The molecular biology method is important technique in the field of Systematics and Ecology of Botany. In this research, I tried to construct the research systems for analyzing genetic variances among closely related taxa or population using the random amplified Polymorphic DNA (RAPD) maker method.1.Analyses for population structure of Myrica rubura in the Yakushima Island.I investigated genetic variances of 77 plants from three population of Myrica rubura, which is characterized by wind pollination and animal seed dispersal. Among 120 of RAPD makers, 8 of dominant and 8of co-dominant were useful to distinct individuals genetically. Using distribution pattern, size, sexuality and genetic type of each individual, seed and pollen dispersal process were estimated. About 2/3 individual of each population were derived from the outside population. Although the effective wind pollination distance was said to be about 10-20 m, Myrica pollen effectively dispersed about 50-440 m.2.Genomic reconstitution in the Australian Compositae, the genus Brachyscome.Though three taxa, B.campylocarpa, B.eriogona and B.smithwhitei are closely resembled in gross morphological features, their genome extremely differs in size. Eighteen RAPD markers, which were amplified specifically from B.eriogona with large genome, were sequenced and used for southern hybridization analyses. Six of them were retrotransposons and transposons, and several copies of them were found in the small genome of B.smithwhite, while they were not detected in the large genome of B.campylocarpa. Remarkable genome size variation in the Brachycome supposed to be derived transposable elements.

分子生物学方法是植物学和生态学领域的重要技术。 In this research, I tried to construct the research systems for analyzing genetic variances among closely related taxa or population using the random amplified Polymorphic DNA (RAPD) maker method.1.Analyses for population structure of Myrica rubura in the Yakushima Island.I investigated genetic variances of 77 plants from three population of Myrica rubura, which is characterized by wind pollination and animal seed dispersal.在120名RAPD制造商中，有8位主导者和8OF共同主导者对不同的个体有用。估计了使用分布模式，大小，性和遗传类型，种子和花粉分散过程。每个人群的大约2/3个个人来自外部人群。尽管据说有效的风授粉距离约为10-20 m，但Myrica花粉在澳大利亚复合症中有效分散了约50-440 m.2。将18个RAPD标记物专门从B. eriogona具有大基因组进行了测序，并用于南部杂交分析。其中六个是逆转座子和转座子，在B.smithwhite的小基因组中发现了几个副本，而在b.campylocarpa的大基因组中未检测到它们。甲状腺肿的显着基因组大小变化，应该是衍生的转座元素。