Expression levels of CD19 and CD22 in autoimmune bullous disease and their effect on antibody production.

自身免疫性大疱性疾病中 CD19 和 CD22 的表达水平及其对抗体产生的影响。

• 批准号: 11670821
• 负责人: INAOKI Makoto
• 金额: $ 2.05万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670821/
• 关键词: bullous pemphigoid; pemphigus; B cell; CD19; CD22; CD21; CD23; autoimmunity; 自己免疫性水疱症; 水疱症類天疱瘡; 尋常性天疱瘡; 落葉状天疱瘡; Bリンパ球

CD19 and CD22 are response regulators of B cells, which might govern balance between tolerance and autoimmunity. To elucidate the role of CD19 and CD22 in development of an autoimmune bullous disease, bullous pemphigoid (BP), we measured the expression levels of CD19, CD22, and other activation markers on B cells from 18 patients with BP.Expression levels of CD19 were normal in BP.Interestingly, expression levels of CD22 on B cells from patients with BP was significantly elevated by 20% compared with those found in normal control subjects, Furthermore, the expression levels of CD22 showed significant inverse correlation with serum IgM levels. Expression levels of CD21 on B cells from patients with BP were significantly elevated compared with those found in normal control subjects. In addition, expression levels of CD23 were significantly elevated compared with those found in normal control subjects. The CD23 expression levels were significantly correlated with serum IgE levels. These results suggest that CD22 and CD23 may play a role in development of BP, by regulating serum immunoglobulin levels. To clarify the relationship between decreased CD22 expression and autoimmunity, we have started analyzing CD22 +/- mice whose B cell shows decreased expression of CD22 by 50%. We are also going to analyze expression levels of CD19 and CD22 on B cells specific for BP antigen.We have measured expression levels of CD19, CD22, CD21 on B cells from 7 patients with pemphigus foliaceus (PF) and 4 patients with patients with pemphigus vulgaris (PV). We found decreased CD19 expression in PF and PV, as well as increased expression of CD21 in PF.We are going to analyze more patients to clarify the relationship between these molecules and serum antibodies.

CD19 和 CD22 是 B 细胞的反应调节因子，可能控制耐受性和自身免疫之间的平衡。为了阐明 CD19 和 CD22 在自身免疫性大疱性疾病、大疱性类天疱疮 (BP) 发展中的作用，我们测量了 18 名 BP 患者的 B 细胞上 CD19、CD22 和其他激活标记物的表达水平。有趣的是，与正常对照受试者相比，BP 患者 B 细胞上 CD22 的表达水平显着升高 20%，此外，CD22的表达水平与血清​​IgM水平呈显着负相关。与正常对照组相比，BP 患者 B 细胞上 CD21 的表达水平显着升高。此外，与正常对照受试者相比，CD23的表达水平显着升高。 CD23表达水平与血清​​IgE水平显着相关。这些结果表明 CD22 和 CD23 可能通过调节血清免疫球蛋白水平在 BP 的发展中发挥作用。为了阐明 CD22 表达下降与自身免疫之间的关系，我们开始分析 CD22 +/- 小鼠，其 B 细胞显示 CD22 表达下降 50%。我们还将分析 BP 抗原特异性 B 细胞上 CD19 和 CD22 的表达水平。我们测量了 7 名落叶型天疱疮 (PF) 患者和 4 名天疱疮患者的 B 细胞上 CD19、CD22、CD21 的表达水平寻常型（PV）。我们发现PF和PV中CD19表达减少，以及PF中CD21表达增加。我们将分析更多患者以阐明这些分子与血清抗体之间的关系。