A role of NO on neuroendocrine system : molecular, pharmacological and neurophysiological approaches

NO 对神经内分泌系统的作用：分子、药理学和神经生理学方法

基本信息

批准号：
11670078
负责人：
UETA Yoichi
金额：
$ 2.3万
依托单位：
University of Occupational and Environmental Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

NO is thought to have important roles as a factor of cross-talk with cell-to-cell communication in not only peripheral system but also central nervous system. The purpose of the present study was to examine the physiological role on the neuroendocrine system that integrates autonomic nervous function and endocrine. Patch-clamp recording from the rat supraoptic nucleus (SON) in vitro demonstrated that inhibitory postsynaptic currents were increased by application of NO donor. For nociceptive stimulation, formalin (5%) or saline was injected subcutaneously (s.c.) into the bilateral hind paws of rats. The expression of the neuronal NO synthase (nNOS) gene in the paraventricular nucleus (PVN) was significantly increased 2 and 6 h after s.c. injection of formalin in comparison with that in untreated and saline injected rats. Food deprivation for 2 days caused downregulation of the expression of the nNOS gene in the PVN and the SON of rats. Central administration of leptin prevented food deprivation-induced that downregulation. In lithium-induced diabetes insipidus rats nNOS mRNA and NADPH-diaphorase activity were increased in the PVN and the SON.The levels of nNOS mRNA and NADPH-diaphorase activity in the PVN and SON of hypertensive Dahl S rats were greater than those in Dahl R rats on a high salt diet. The antihypertensive drugs, either nicardipine or captopril were administered to the Dahl S rats for 2 weeks beginning at 2 weeks after the start of the high salt diet. The nNOS mRNA in the PVN and SON of Dahl S rats given a high salt diet was not upregulated by treatment with nicardipine, while the nNOS mRNA in salt-loaded Dahl S rats was greater upregulated by treatment with captopril to a greater than without the antihypertensive drug. These results suggest that NO may have various physiological roles in the neuroendocrine system.

人们认为NO具有重要的作用，这是与细胞到细胞通信不仅在外围系统中，而且是中枢神经系统中的细胞之间通信的一个因素。本研究的目的是检查整合自主神经功能和内分泌的神经内分泌系统上的生理作用。从大鼠上核（SON）中记录的斑块钳记录体外表明，通过应用无供体增加了抑制性突触后电流。对于伤害性刺激，将福尔马林（5％）或盐水皮下注射（S.C.）注入大鼠双侧后爪中。在S.C.后，神经元NO合酶（NNOS）基因的表达显着增加了2和6小时。与未经处理和盐水注入的大鼠相比，注射福尔马林。食物剥夺2天导致NNOS基因在PVN和大鼠儿子中的表达下调。瘦素的中央给药阻止了食物剥夺引起的下调。在锂诱导的糖尿病中，PVN和SON中有NNOS mRNA和NADPH-DIAPHORASE的活性增加。NNOSmRNA和NADPH-DIAPHORASE的水平在PVN中的水平和高血压DAHL S大鼠的儿子大于Dahl R R R R R r R r R r r r r r r r r y于此高盐饮食的大鼠。从高盐饮食开始后2周开始，将抗高血压药物，即尼卡迪平或卡托普利施用2周。在PVN中的NNOS mRNA和Dahl s大鼠的儿子在高盐饮食中没有被尼卡迪平的治疗上调，而在盐中加载的dahl s大鼠中的NNOS mRNA通过catchopril的治疗更大地上调至carteropril，比没有抗高型药品。这些结果表明，没有在神经内分泌系统中具有各种生理作用。