Molecular linkage map construction and mapping genes for useful traits in Asiatic hybrid lily

亚洲杂交百合有用性状分子连锁图谱构建及基因定位

基本信息

批准号：
11660031
负责人：
YAMAGISHI Masumi
金额：
$ 1.92万
依托单位：
Shimane University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660031/
关键词：
Asiatic hybrid lily Anthocyanin Carotenoids Chalcone synthase (CHS)Dihydroflavonol 4-reductase (DFR)Lilium hybrids Linkage map QTL mapping Asiatic hybrid lily Myb asiatic hybrid lily Lilium sp RAPDマーカー ISSRマーカー cDNAライブラリー花

项目摘要

To understand the genetic background of two floral anthocyanin pigmentation traits, anthocyanin pigmentation in the flower tepals and spot formation, in the Asiatic hybrid lily (2n=24), segregation of the two traits among 96 F_1 plants derived from a cross between commercial cultivars 'Montreux' and 'Connecticut King' were investigated. 'Montreux' has anthocyanin pigmentation in the tepals with many spots, and 'Connecticut King' has flowers with carotenoid pigmentation without spots. The F_1 plants with or without anthocyanin pigment in the tepals segregated with a 1 : 1 segregation ratio, indicating that a single gene controls anthocyanin pigmentation in the tepals. The number of spots per square centimeter of all tepals showed continuous distribution in the F_1 plants. To map the loci for the two anthocyanin pigmentation traits, molecular linkage maps in the Asiatic hybrid lily were constructed using a double pseudo-testcross strategy with the same F_1 plants used for phenotypic evaluation and 212 PCR-based DNA markers. The trait for anthocyanin pigmentation in tepals was used as a trait marker. The map of 'Montreux' comprised 95 markers in 26 linkage groups, and the map of 'Connecticut King' used 119 markers in 24 linkage groups. The total map lengths were 867.5 and 1114.8 cM, respectively. The trait locus for anthocyanin pigmentation in the tepals was between markers ASR35-180 and P506-40 in linkage group 1 of the 'Montreux' map with map distance 1.2 cM and 2.6 cM, respectively. A single point analysis of quantitative trait loci (QTL) for tepal spot number identified two putative QTLs in linkage groups 1 and 19 of the 'Connecticut King' map. One putative QTL in the linkage group 19 explained 64 % of the total phenotypic variation. Because both putative QTLs were mapped on the linkage map of 'Connecticut King' that has no spots, dominant alleles of them might suppress spot formation.

要了解两个花花青素色素沉着性状的遗传背景，花旋风中的花青素色素和斑点形成，在亚洲杂种百合（2n = 24）中（2n = 24），在96个F_1植物中的两个特征隔离了两种特征。调查了蒙特勒'和“康涅狄格州国王”。 “ Montreux”在带有许多斑点的Tepals中具有花青素色素沉着，并且“康涅狄格州国王”的花朵上有带类胡萝卜素色素沉着的花，没有斑点。 f_1的植物植物中有或没有花色苷色素的植物植物以1：1的隔离比隔离，表明单个基因控制着阵风中的花青素色素沉着。所有阵风的每平方厘米的斑点数量显示在F_1植物中的连续分布。为了绘制两个花生素色素沉着性状的基因座，使用双重伪测试策略构建了亚洲杂种百合中的分子连锁图，该策略与用于表型评估的相同F_1植物和基于212 PCR的DNA标记。花色蛋白色素化的特征用作特质标记。 “蒙特勒”的地图包括26个连锁组的95个标记，“康涅狄格州国王”的地图在24个连锁组中使用了119个标记。总地图长度分别为867.5和1114.8厘米。在Tepals中，在标记ASR35-180和p506-40中，在链接组1的标记中，链接距离1.2 cm和2.6 cm的链接组1分别在链接组1 ASR35-180和p506-40之间的性状位点。链球数的定量性状基因座（QTL）的单点分析在“康涅狄格州国王”地图的1和19中确定了两个推定的QTL。连锁组19中的一个推定QTL解释了总表型变化的64％。由于两个推定的QTL都均在没有斑点的“康涅狄格州国王”的链接图上映射，因此它们的主要等位基因可能会抑制斑点形成。