Behavioralpharmacological study on memory in rats

对大鼠记忆力的行为药理学研究

基本信息

批准号：
60571098
负责人：
ANDO Kiyoshi
金额：
$ 0.77万
依托单位：
Central Institute for Experimental Animals
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60571098/
关键词：
Rat Delayed discrimination Y maze Operant chamber Scopolamine Physostigmine AF64A

项目摘要

Delayed discrimination behavior was established in a Y-maze situation and in 2-lever operant chamber situations in rats where a discriminative stimulus light either on the right side or the left side was presented for a brief period. Running responses or responses on the lever to the lighted side were reinforced with food pellets. After establishment of the above behavior, the percentage of correct choice responses to the discriminative stimulus side was observed with various delay times (0-32 sec) after extinguishing the discriminative light. More than 80% was attained just after termination of the discriminative light (delay = 0 sec) while the longer the delay time elapsed, the lower the percentage. In comparison with saline control, scopolamine at 0.06 mg/kg, sc, physostigmine at 0.06 mg/kg, sc, arecoline at 1 mg/kg, sc, and diazepam at 1 mg/kg, sc, decreased the percentage at various delay times including 0 sec. AF-64A at 6 nmol per rat was given intraventricularly to the rats which had showed established delayed discrimination responses in Y-maze situation. The responses were disrupted for more than 2 weeks after the administration while the responses in rats administered saline intraventriculary were not disrupted. Testing the improving effects of drugs on the disrupted responses by AF-64A was remained for further study. In conclusion, the present behavioral methods may be useful for evaluating drug effects on functions relating memory processes in animals and AF-64A may also be useful for studying memory related dysfunctioning in relation with cholinergic nervous system.

在 Y 迷宫情况和 2 杆操作室情况下，大鼠建立了延迟辨别行为，其中在右侧或左侧呈现短暂的辨别刺激光。跑步反应或控制杆对发光侧的反应通过食物颗粒得到加强。建立上述行为后，在熄灭辨别光后以不同的延迟时间（0-32秒）观察对辨别刺激侧的正确选择反应的百分比。在判别光终止后（延迟 = 0 秒）就达到了 80% 以上，而延迟时间越长，百分比越低。与盐水对照相比，东莨菪碱 0.06 mg/kg，皮下注射、毒扁豆碱 0.06 mg/kg，皮下注射、槟榔碱 1 mg/kg，皮下注射和地西泮 1 mg/kg，皮下注射，在不同延迟时间下百分比均降低包括 0 秒。将每只大鼠 6 nmol 的 AF-64A 脑室内给予在 Y 迷宫情况下表现出延迟辨别反应的大鼠。给药后反应被中断超过2周，而脑室内注射生理盐水的大鼠的反应没有中断。测试药物对 AF-64A 破坏的反应的改善效果仍有待进一步研究。总之，本行为方法可用于评估药物对动物记忆过程相关功能的影响，并且 AF-64A 也可用于研究与胆碱能神经系统相关的记忆相关功能障碍。