Phylogeny and evolution of amitochondriate eukaryotes

线粒体真核生物的系统发育和进化

基本信息

批准号：
10836017
负责人：
YANO Taka-aki
金额：
$ 2.18万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10836017/
关键词：
Early Evolution of Eukaryotes Mitochondria Protists Alignment Molecular phylogeny Ribosomal Proteins Heat Shock Protein 70 Peptide Elongation Factors ヴァリンtRNA合成酵素イソロイシンtRNA合成酵素

项目摘要

In order to elucidate early evolution of eukaryotes, we have been studying phylogenetic relationship aimng protists including arnitochondriate ones with the use of multiple molecular sequence data. In this project, we established the methods to get enough DNA needed for gene analyses of the protists, including Giardia lamblia (Diplomonads), Trichon2onas vaginalis, T. tenax (Trichomonads), Entamoeba histolytica (Entamoebidae), Trypanosoma cruzi (Euglenoids), Biastocystis hominis, Encephalitozoon cuniculi, E.heilein, Glugeaplecogiossi (Microsporidia), and Mastigarnoeba balamuthi. On the basis of the DNA of these protists, we cloned and sequenced the genes encoding isoleucyl- and valyl-tRNA synthetases, ribosomal proteins, 7OkDa heat shock proteins, and peptide elongation factors, the sequences of which had yet been detennined. Phylogenetic analyses including these original data suggested 1) that all the sequences from amitochondriate protists clearly show the sequence feature of typical eukaryotic type, 2) that all of the four amitochondriate protists lineages, Diplomonads, Tricbomonads, Entarnoebidae, and Microsporidia lost their mitochondria secondarily during their evolution, and 3) that B. hominis is involved in Stramenopiles and the closest relative of the Stramenopiles is shred by Alveolata.

为了阐明真核生物的早期演变，我们一直在研究使用多个分子序列数据，包括arnitochriate的系统发育关系目标。 In this project, we established the methods to get enough DNA needed for gene analyses of the protists, including Giardia lamblia (Diplomonads), Trichon2onas vaginalis, T. tenax (Trichomonads), Entamoeba histolytica (Entamoebidae), Trypanosoma cruzi (Euglenoids), Biastocystis hominis，脑脑cuniculi，e.heilein，glugeaplecogiossi（Microsporidia）和mastigarnoeba balamuthi。在这些生物的DNA的基础上，我们克隆并测序了编码异戊酸和脱甲基-TRNA合成酶，核糖体蛋白，7OKDA热休克蛋白以及肽伸长因子的基因，其序列尚未被确定。 Phylogenetic analyses including these original data suggested 1) that all the sequences from amitochondriate protists clearly show the sequence feature of typical eukaryotic type, 2) that all of the four amitochondriate protists lineages, Diplomonads, Tricbomonads, Entarnoebidae, and Microsporidia lost their mitochondria secondarily during它们的进化，以及3）hominis参与杂质的hominis，而斯特雷莫索的最接近的亲戚被肺泡切碎。