Dynamic organization of chromosomal DNA in Escherichia coli.

大肠杆菌中染色体 DNA 的动态组织。

• 批准号: 10680655
• 负责人: NIKI Hironori
• 金额: $ 2.18万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680655/
• 关键词: Prokaryote; cell division; FISH; nucleoid; partitioning; segregation; 染色体; 細胞内局在; 染色体逆位; 大腸菌; 移動; 複製起点; 複製終点; セントロメア; 原核細胞

We have revealed the subcellular localization of different DNA segments that are located at approximately 〜230-kb intervals on the Escherichia coli chromosome using fluorescence in situ hybridization (FISH). The series of chromosome segments is localized within the cell in the same order as the chromosome map. The large chromosome region including oriC shows similar localization patterns, which we call the Ori domain. In addition, the localization pattern of the large segment including dif is characteristic of the replication terminus region. The segment also shows similar localization patterns, which we call the Ter domain. In newborn cells, Ori and Ter domains of the chromosome are differentially localized near opposite cell poles. Subsequently, in the B period, the Ori domain moves toward mid-cell before the initiation of replication, and the Ter domain tends to relocate at mid-cell. An inversion mutant, in which the Ter domain is located close to oriC, shows abnormal subcellular localization of ori and dif segments, resulting in frequent production of anucleate cells. These studies thus suggest that the E. coli chromosome is organized to form a compacted ring structure with the Ori and Ter domains; these domains participate in the cell cycle- dependent localization of the chromosome.

我们利用荧光原位杂交 (FISH) 揭示了位于大肠杆菌染色体上约 230 kb 间隔的不同 DNA 片段的亚细胞定位。这一系列染色体片段以与细胞内相同的顺序定位。包括oriC的大染色体区域显示出相似的定位模式，我们称之为Ori结构域，此外，包括dif的大片段的定位模式是复制末端区域的特征。定位模式，我们称之为 Ter 结构域，在新生细胞中，染色体的 Ori 和 Ter 结构域差异定位在相反的细胞极附近，随后，在 B 期，Ori 结构域在复制开始之前向细胞中部移动， Ter 结构域倾向于重新定位在细胞中部，其中 Ter 结构域位于 oriC 附近，显示 ori 和 diff 片段的异常亚细胞定位，导致无核细胞的频繁产生。这大肠杆菌染色体组织形成具有 Ori 和 Ter 结构域的致密环结构；这些结构域参与染色体的细胞周期依赖性定位。

项目成果

Onogi,T., H.Niki, M.Yamazoe and S.Hiraga: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli"Mol Microbiol. 31. 1775-1782 (1999)

Onogi,T.、H.Niki、M.Yamazoe 和 S.Hiraga：“大肠杆菌活细胞中 SeqA-Gfp 融合体的组装和迁移”Mol Microbiol。

Hirano,M.: "Autorequlation of the partition genes of mini-F plasmid and the intracellular localization of their products in Escherichia coli." Mol.Gen.Genet.257. 392-403 (1998)

Hirano,M.：“迷你 F 质粒的分区基因的自动调节及其产物在大肠杆菌中的细胞内定位。”

仁木 宏典: "細菌における分子細胞生物学の新展開ー染色体分配と細胞分裂"蛋白質核酸酵素. 45. 153-163 (2000)

Hironori Niki：“细菌分子细胞生物学的新进展——染色体分离和细胞分裂”蛋白质核酸酶45。153-163（2000）。

Niki, H.: "Subcellular localization of plasmids containing the oriC region of Escherichia coli chromosome, with or without the sopABC partitioning system"Mol. Microbiol.. 34. 498-503 (1999)

Niki, H.：“含有大肠杆菌染色体 oriC 区域的质粒的亚细胞定位，有或没有 sopABC 分配系统”Mol。

Hirano, M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellular localization of their products in escherichia coli"Mol. Gen. Genet.. 257. 392-403 (1998)

Hirano, M.：“小 F 质粒分区基因的自动调节及其产物在大肠杆菌中的细胞内定位”Mol。