Effect of spin trap agent under oxidative stress by high-dose oxygen exposure

自旋捕获剂在高剂量氧气暴露氧化应激下的影响

基本信息

批准号：
10680520
负责人：
SAITO Kieko
金额：
$ 2.24万
依托单位：
University of Shizuoka
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680520/
关键词：
PBN spin trapping oxidative stress nitric oxide high pressure oxygen ESR Free radical Nitric oxide PBN(N-t-α-phenyl-butylnitrone)

项目摘要

We have been suggested that spin trap agent, N-tert-buttyl-α-Phenylnitrone (PBN) may be a source of nitric oxide other than serving an antioxidant under oxidative stress. In this study we determined the nitric oxide (NO) formation to characterize the effect of PBN under high pressure oxygen. NO formation of mice treated with high pressure oxygen was measured using an Nox analyzer and an electron spin resonance (ESR). An oxidative product of No, nitrite formation of the blood of mice under high pressure atmosphere. NOS (nitric oxide synthase) inhibitor, L-NAME, decreased the NO formation of the mice to the normal level of NO, indicating that the NO was formed via NOS. ESR spectra of liver and brain of the mice were measured inn the both control and experimental mice using Fe(DETC)2 as a NO trapper. Signal of NO complex was observed in the liver of the mice under the condition of high pressure oxygen. Furthermore, effect of PBN administration was investigated. Nitrite formation in the blood of the mice treated with PBN was decreased under the condition of high pressure oxygen. In addition, the intensity of NO complex was weaker in the PBN-treated mice than the non-treated mice on ESR study.The results indicate that PBN suppressed NO formation under high pressure oxygen. PBN as a source of nitric oxide may control physiological functions under the condition of high-dose oxygen.

我们认为，自旋捕获剂 N-叔丁基-α-苯基硝酮 (PBN) 可能是一氧化氮的来源，而不是氧化应激下的抗氧化剂。在这项研究中，我们确定了氧化应激下一氧化氮 (NO) 的形成。使用 Nox 分析仪和电子自旋共振 (ESR) 来表征高压氧处理小鼠的 NO 形成。高压环境下小鼠血液中亚硝酸盐的形成，NOS（一氧化氮合酶）抑制剂，L-NAME，将小鼠的NO形成降低到NO的正常水平，表明NO是通过NOS的ESR谱形成的。使用 Fe(DETC)2 作为 NO 捕集剂对对照小鼠和实验小鼠的肝脏和大脑进行测量，在高压氧条件下在小鼠肝脏中观察到 NO 复合物的信号。 PBN 管理是研究发现，在高压氧条件下，PBN 处理的小鼠血液中亚硝酸盐的形成有所减少，此外，在 ESR 研究中，PBN 处理的小鼠中 NO 复合物的强度较未处理的小鼠更弱。结果表明，PBN在高压氧下抑制NO的形成。PBN作为一氧化氮的来源可以在高剂量氧条件下控制生理功能。