Cytotoxicity of polymerization-initiators in dentistry and prevention with antioxodants

牙科聚合引发剂的细胞毒性和抗氧化剂的预防

• 批准号: 10671842
• 负责人: FUJISAWA S.
• 金额: $ 1.79万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671842/
• 关键词: polymerization initiators; antioxidants; eugenol; radical scavenging; polymer radicals; dimer; cytotoxicity; reactive oxgen species; 2-アリル-4-メトキシフェノール; フェノキシルラジカル; DPPHラジカル; オルトビスフェノール; ラジカル禁止効果; フェノールラジカル; アゾビスイソブチロニトリル; カップリング化

To clarify the cytotoxic activity of polymerization initiators in the resin system, the cytotoxicity of camphorquinone (CQ), benzyl and 9-fluorenone (9F) against human tooth pulp fibroblasts, gingival fibroblasts and human submandibular tumor cells were studied. The cytotoxicity declined in the order : 9F>>Benzyl>CQ.The polymerizability of triethyleneglycoldimethacrylate of CQ was the highest. Taken together, CQ was the best initiator. Radical scavenging effects of eugenol-related compounds in benzoyl peroxide-methyl methacrylate system were studied, indicating that the radical scavenging activity of dimers (i.e. bis-eugenol, bis-MMP, bis-BHA ns bis-BMP) was higher than that of monomers (eugenol, 2-methoxy-4-methylphenol (MMP), 2-t-butyl-4-methoxy phenol (BHA) and 2-t-butyl-4-methylphenol (BMP)). In contrast, the interaction activity of polymer radicals with monomers showed a higher activity than that of dimers and BMP was the most effective. In 2-ally-4-X-phenols, 2-allyl-4-methoxyphenol was the most effective.Antioxidants such as eugenol related compounds were converted themselves into phenoxyl radicals during scavenging radicals and in addition, reactve oxygen radicals were produced. They caused cytotoxicity via apoptosis induction.

为了阐明树脂系统中聚合启动器的细胞毒性活性，研究了樟脑酮（CQ），苄基和9-氟烯酮（9F）对人牙浆成纤维细胞，牙龈成核细胞的细胞毒性。细胞毒性在以下顺序下降：9f >>苄基> cq。CQ的三乙二烯甲基丙烯酸甲酯的聚合性最高。综上所述，CQ是最好的发起人。研究了二烯醇相关化合物在苯甲酰氧化甲基甲基丙烯酸甲酯系统中的根本清除作用，表明二聚体的根本清除活性（即BIS-EUGENOL，BIS-MMP，BIS-MMP，BIS-MMP，BIS-BHA NS NS BIS-BMP）比单体高。 （Eugenol，2-甲氧基-4-甲基苯酚（MMP），2-T-T-叔丁基-4-甲氧基苯酚（BHA）和2-T-T-叔丁基-4-甲基苯酚（BMP））。相反，聚合物自由基与单体的相互作用活性比二聚体和BMP的活性更高。在2-含-4-X-苯酚中，2-酰基-4-甲氧基酚是最有效的。在清除自由基过程中，将抗氧化酚与丁香酚相关的化合物（例如相关的化合物）被转化为苯氧基自由基，此外，产生了反应氧自由基。它们通过凋亡诱导引起细胞毒性。

项目成果

Fujisawa 他4名: "Cytotoxicity of photosensitizers comphorquinone and 9-fluorenone with visible light irradiation on a human submandibular-duct cell line in vitro" Archives of Oral Biology. 43. 73-81 (1998)

Fujisawa 和其他 4 人：“体外可见光照射下光敏剂康佛醌和 9-芴酮对人下颌下导管细胞系的细胞毒性”口腔生物学档案 43. 73-81 (1998)。

Fujisawa 他8名: "Radical modulating and cytotoxic activity of synthesized eugenol-related compounds"Anticancer Res. 20. 2955-2960 (2000)

Fujisawa 等 8 人：“合成的丁子香酚相关化合物的自由基调节和细胞毒性活性”Anticancer Res. 20. 2955-2960 (2000)

Fujisawa S.他1名: "^1H-NMR spectroscopic studies of the interaction of thymol with phospholipid liposomes" Dent. Mater. J. 17・2. 139-147 (1998)

Fujisawa S.等人：“百里酚与磷脂脂质体相互作用的^1H-NMR光谱研究”Dent Mater. 17・2 (1998)。

Fujisawa 他2名: "Cytotoxicity of methyl methacrylate (MMA) and related compounds and their interaction with dipalmitoyl phosphatidylcholine (DPPC) liposomes as a mosletor biomembrance"Oral Diseases. 6. 215-221 (2000)

Fujisawa 和其他 2 人：“甲基丙烯酸甲酯 (MMA) 和相关化合物的细胞毒性及其与作为 mosletor 生物膜的二棕榈酰磷脂酰胆碱 (DPPC) 脂质体的相互作用”口腔疾病。

Fujisawa S 他8名: "Radical intensity and cytotoxicity of butylated hydroxyanisole and its orthobisphenol dimer"Anticancer Res. 19(513). 5947-5952 (1999)

Fujisawa S 等 8 人：“丁基化羟基茴香醚及其邻位双酚二聚体的自由基强度和细胞毒性”Anticancer Res 19(513) (1999)。