A Study of a Quantification of Organs Perfusion with Animal Model Using Nuclear Medicine Technique : Factor of Absorption and Scatter on Gamma-Ray

利用核医学技术对动物模型器官灌注进行定量研究：伽马射线吸收和散射因素

基本信息

批准号：
10670836
负责人：
ONOGUCHI Masahisa
金额：
$ 0.77万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

It's reported that the duodenum and the small intestine of the activity excreted from the liver and gallbladder can lead to interference with the myocardial uptake. To study how precisely images reflect tracer uptake in a myocardium, as fundamental experiment to examine the influence that accumulation of the liver and alimentary tract (except esophagus) of the activity excreted from the liver gave to the myocardium image in the technetium-99m myocardium perfusion tracer, we administered ィイD199mィエD1Tc-tetrofosmin (following, Tetrofosmin) to a normal rat and compared with measured value obtained by a gamma camera and an actual survey value after the organs extraction. Methods : Thirty-three rats were measured for 5, 10, 15, 30, 45, 60, 90 and 120min after the administration of Tetrofosmin. The accumulated rates for the myocardium, the lung, the liver, the duodenum, the small intestine, the rectum and the blood with a well-type scintillation counter (WC) were assessed as a percentage of t … More he injected dose per gram of tissue (%ID/g). The accumulated rates for each organ with the planar imaging (PI) were assessed as a percentage of the injected dose per pixel (%ID/pixel) establishing regions of interest (ROIs) in each organ. The accumulated rates for each organ, the PI-to-WC ratio and the heart-to-organ ratios calculated by PI and WC were compared. Results : The accumulated rate for the heart with WC increased until 30 min (1.671%±0.306%) after the injection, and subsequently decreased. On the other hand, peak of the accumulated rate with PI was shown at 15 min (1.814%±0.518%) after the injection. Particularly a difference of both was higher in 10 min (PI/WC ratio : about 1.6 times) of the early phase. The accumulated rate for the lung with WC was maximum at 15 min (0.870%±0.312%) after the injection. The accumulated rate with PI, however, showed the maximum value at 10 min (1.139%±0.376%), and a variation of the accumulated rate was hardly recognized 10 min later. The accumulated rate for the liver with WC was hardly shown a marked variance from 5min (0.768%±0.193%) to 30min (0.692%±0.273%), and a tendency of decrease was showed slowly. On the other hand, the accumulated rate with PI was more highly than that with WC in the whole, and high values were showed in 10 min (PI/WC ratio : about 3.3 times) and 15 min (PI/WC ratio : about 2.3 times) in particular. The accumulated rate for the duodenum and the upper small intestine was maximum in 10 min (1.228%±0.602%, 2.245%±1.936%) after the injection, and approximately 90% of ィイD199mィエD1Tc-tetrofosmin were the duodenum excrements. Conclusion : The accumulated rates for each organ with PI were showed higher than that with WC. The difference of both, particularly, was stronger in early phase after the injection. The accumulated rate for the heart with PI was shown significant higher than that with WC at 10 min. The interference of Compton scatter from the liver, duodenum and small intestine was suggested as one of these causes. Less

据报道，十二指肠和小肠从肝脏和胆囊排出的活性会导致对心肌摄取的干扰，以研究图像如何精确地反映心肌中示踪剂的摄取，作为检测示踪剂积累的影响的基础实验。肝脏和消化道（食道除外）从肝脏排出的活性在 Technetium-99m 心肌中形成心肌图像灌注示踪剂，我们给正常大鼠注射D199m2Tc-替曲膦（以下简称替曲膦），并与伽马相机测量值和器官摘除后的实际测量值进行比较。方法：对33只大鼠进行5次、10次、10次测量。给予替曲膦后15、30、45、60、90和120分钟累积。使用井型闪烁计数器 (WC) 评估心肌、肺、肝脏、十二指肠、小肠、直肠和血液的比率，以每克组织注射剂量的百分比进行评估（平面成像（PI）的每个器官的累积速率被评估为每个像素注射剂量的百分比（％ID/像素），建立感兴趣区域（ROI）。比较每个器官的累积率、PI 与 WC 比率以及通过 PI 和 WC 计算的心脏与器官比率结果：WC 心脏的累积率增加直至 30 分钟（1.671%）。 ±0.306%)，随后下降；另一方面，PI的累积率峰值出现在注射后15分钟(1.814%±0.518%)。特别是在早期10分钟(PI/WC比：约1.6倍)两者的差异较高，在注射后15分钟时肺部的WC累积率最大(0.870％±0.312％)。然而，PI的速率在10分钟时显示最大值（1.139％±0.376％），并且10分钟后几乎无法识别累积速率的变化。 WC的肝脏累积率从5分钟（0.768%±0.193%）到30分钟（0.692%±0.273%）几乎没有表现出明显的变化，并且呈现出缓慢下降的趋势。整体而言，PI比WC更高，在10分钟（PI/WC比率：约3.3倍）和15分钟（PI/WC比率：约3.3倍）和15分钟（PI/WC特别是十二指肠和小肠上部的累积率在注射后10分钟内达到最大（1.228%±0.602%、2.245%±1.936%），约为iiD199m PI D1Tc的90%。结论：十二指肠排泄物中-替曲膦的累积率较高。特别是在注射后的早期阶段，两者的差异更大。在 10 分钟时，心脏的累积率明显高于 WC。来自肝脏的康普顿散射的干扰。十二指肠和小肠被认为是这些原因之一。