A Study of a Quantification of Organs Perfusion with Animal Model Using Nuclear Medicine Technique : Factor of Absorption and Scatter on Gamma-Ray

利用核医学技术对动物模型器官灌注进行定量研究：伽马射线吸收和散射因素

• 批准号: 10670836
• 负责人: ONOGUCHI Masahisa
• 金额: $ 0.77万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670836/
• 关键词: ィイD199mィエD1Tc-tetrofosmin; Myocardial uptake; Planar images; Scintillation counter; Rat; ガンマカメラ; テトロフオスミン; 心筋血流; 定量; 吸収; 散乱線

It's reported that the duodenum and the small intestine of the activity excreted from the liver and gallbladder can lead to interference with the myocardial uptake. To study how precisely images reflect tracer uptake in a myocardium, as fundamental experiment to examine the influence that accumulation of the liver and alimentary tract (except esophagus) of the activity excreted from the liver gave to the myocardium image in the technetium-99m myocardium perfusion tracer, we administered ィイD199mィエD1Tc-tetrofosmin (following, Tetrofosmin) to a normal rat and compared with measured value obtained by a gamma camera and an actual survey value after the organs extraction. Methods : Thirty-three rats were measured for 5, 10, 15, 30, 45, 60, 90 and 120min after the administration of Tetrofosmin. The accumulated rates for the myocardium, the lung, the liver, the duodenum, the small intestine, the rectum and the blood with a well-type scintillation counter (WC) were assessed as a percentage of t … More he injected dose per gram of tissue (%ID/g). The accumulated rates for each organ with the planar imaging (PI) were assessed as a percentage of the injected dose per pixel (%ID/pixel) establishing regions of interest (ROIs) in each organ. The accumulated rates for each organ, the PI-to-WC ratio and the heart-to-organ ratios calculated by PI and WC were compared. Results : The accumulated rate for the heart with WC increased until 30 min (1.671%±0.306%) after the injection, and subsequently decreased. On the other hand, peak of the accumulated rate with PI was shown at 15 min (1.814%±0.518%) after the injection. Particularly a difference of both was higher in 10 min (PI/WC ratio : about 1.6 times) of the early phase. The accumulated rate for the lung with WC was maximum at 15 min (0.870%±0.312%) after the injection. The accumulated rate with PI, however, showed the maximum value at 10 min (1.139%±0.376%), and a variation of the accumulated rate was hardly recognized 10 min later. The accumulated rate for the liver with WC was hardly shown a marked variance from 5min (0.768%±0.193%) to 30min (0.692%±0.273%), and a tendency of decrease was showed slowly. On the other hand, the accumulated rate with PI was more highly than that with WC in the whole, and high values were showed in 10 min (PI/WC ratio : about 3.3 times) and 15 min (PI/WC ratio : about 2.3 times) in particular. The accumulated rate for the duodenum and the upper small intestine was maximum in 10 min (1.228%±0.602%, 2.245%±1.936%) after the injection, and approximately 90% of ィイD199mィエD1Tc-tetrofosmin were the duodenum excrements. Conclusion : The accumulated rates for each organ with PI were showed higher than that with WC. The difference of both, particularly, was stronger in early phase after the injection. The accumulated rate for the heart with PI was shown significant higher than that with WC at 10 min. The interference of Compton scatter from the liver, duodenum and small intestine was suggested as one of these causes. Less

据报道，肝脏和胆囊超过的十二指肠和小肠可导致干扰心肌摄取。为了研究心肌中的示踪剂的精确图像，作为基本实验，以研究肝脏和饮食症的积累（除食管外）的影响，从肝脏中超出了肝脏给予心肌图像在Technetium-99m -99m的心肌心肌灌注式TRACER中给予了d199mie d199mie d199mie d199mie d199mie d199mie d199mie d199mie d199mie d1cctmin正常大鼠，并将其与通过伽马相机获得的测量值和器官提取后的实际调查值进行了比较。方法：在给药四脂蛋白后，测量了5、10、15、30、45、60、90和120分钟的5、10、15、30、45、60、90和120分钟的大鼠。心肌，肺，肝脏，十二指肠，小肠，直肠和血液中带有良好型闪烁式计数器（WC）的累积发生率被评估为T…更高的剂量，他每克每克组织（％id/g）注射剂量。将每个器官的累积速率与平面成像（PI）评估为每个像素（％ID/Pixel）在每个器官中建立利益区域（ROI）的注射剂量的百分比。比较了每个器官的累积速率，PI和WC计算得出的心与器官比率。结果：注射后，WC的心脏的累积率增加到30分钟（1.671％±0.306％），随后降低。另一方面，注射后15分钟（15分钟（1.814％±0.518％）显示了累积率的峰值。尤其是两者的差异在10分钟内（PI/WC比率：约1.6倍）的早期差异较高。注射后15分钟（0.870％±0.312％）的WC肺的累积率最大。但是，PI的累积速率显示出10分钟时的最大值（1.139％±0.376％），并且在10分钟后几乎无法确认累积速率的变化。 WC肝脏的累积速率几乎没有显示出明显的差异，从5分钟（0.768％±0.193％）到30分钟（0.692％±0.273％），并且降低趋势缓慢。另一方面，PI的累积速率总体上比WC高，并且在10分钟（PI/WC比：约3.3次）和15分钟（PI/WC比率：约2.3倍）中显示了高值。注射后，十二指肠和上小肠的累积速率最大为10分钟（1.228％±0.602％，2.245％±1.936％），约为DuododeNum the DuododeNum。结论：每个带PI的器官的累积速率比WC高。尤其是两者的差异在注射后的早期阶段都更强。使用PI的心脏的累积速率显示出比在10分钟时WC高的显着高。康普顿从肝脏，十二指肠和小肠的干扰被认为是这些原因之一。较少的

项目成果

小野口昌久: "99mTC-tetrofosmin心筋画像はどの程度真の取り込みを反映しているか?―ラットを用いたガンマカメラによる測定と実測値の対比―"北陸循環核医学研究会記録集. 31. 5-6 (1999)

Masahisa Onoguchi：“99mTC-替曲膦心肌图像在多大程度上反映了真实的摄取？ - 伽马相机测量值与大鼠实际测量值的比较 -” 北陆循环核医学研究组记录 31. 5-6 (1999)。

Onoguchi, Masahisa: "Biokinetics of Technetium-99m-Tetrofosmin Myocardial Perfusion Imaging Agent in Rats : Comparison between the Values from Planar Images and Excised Organs"Memoirs Health Sci Med Kanazawa Univ. 23. 47-55 (1999)

Onoguchi，Masahisa：“大鼠体内 Technetium-99m-Tetrofosmin 心肌灌注成像剂的生物动力学：平面图像和切除器官的值之间的比较”回忆录健康科学医学金泽大学。

小野口昌久: "ラットにおける心筋四流製剤99mTc-tetrofosminの体内分布―プラナー像と摘出臓器による値の対比―"金沢大学医学部保健学科紀要. 23・2. 47-55 (1999)

小野口正久：“心肌四流制剂99mTc-替曲膦在大鼠体内的分布-平面图像与提取器官的值的比较-”金泽大学医学院健康科学系通报23, 2. 47。 -55 (1999)

小野口昌久: "^<99m>Tc-Tetrofosmin心筋画像はどの程度真の取り込みを反映しているか?-ラットを用いたガンマカメラによる測定と実測値の対比-"北陸循環器核医学研究会記録集. 31. 5-6 (1999)

小野口正久：“^<99m>Tc-替曲膦的心肌图像在多大程度上反映了真实的摄取？-使用大鼠的伽马相机测量与实际测量的比较-”北陆心血管核医学研究组记录31. 5。 -6 (1999)

Onoguchi, Masahisa: "How precisely Myocardial Perfusion images reflect racer uptake? : Comparison between the Values from Planar Images and Excised Organs in Rats"Hokuriku Nuclear Medicine Circulation Conference. 31. 5-6 (1999)

Onoguchi，Masahisa：“心肌灌注图像如何精确地反映赛车摄取？：平面图像和大鼠切除器官的值之间的比较”北陆核医学循环会议。