Research on the manifestation mechanism of Parkinsonian tremor : On the role of serotonergic neuron of raphe nucleus.

帕金森震颤的表现机制研究：中缝核5-羟色胺能神经元的作用。

• 批准号: 10470283
• 负责人: TAKAHASHI Toshio
• 金额: $ 3.78万
• 依托单位: HIROSAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470283/
• 关键词: Parkinson's disease; resting tremor; raphe nuclei; serotonin; clopamine; experimental tremor; MPTP

PURPOSE: The main lesion of Parkinson's disease is the selective neuronal death of the nucleus niger (clopaminergic neuron) and locus ceruleus of the midbrain (noradrenergic neuron). However, we cannot necessarily explain all the manifestations and mechanism of resting tremor by these sites of damage alone. The ventromedial tegmentum (VMT) is known as the region that includes Parkins-onian tremor in the monkey. We have reported that great numbers of cells in the nucleus niger and dorsal raphe nucleus were labeled by the local injection of horseraclish peroxidase into VMT in both of monkeys and cats . It is the purpose of this research to clarify that damages or not only the nucleus niger but also raphe nucleus serotonergic neurons are involved in the manifestation of tremor. METHOD : Fifteen mature cats were divided into two groups : one is a coagulation group of the dorsal raphe nucleus, and the other is a non-coagulation group. In the coagulation group, damage of the nucleus niger do … More pamine system is induded by MPTP, after the destruction of the dorsal raphe nucleus using a stereotactic coagulation technique. In both groups, MPTP (3 mg/kg/day) was injected intramuscularly every other day 8〜14 times in total. The reactions after injection were then recorded and analyzed in video and electromyogram. The coagulated lesions were confirmed in the serial-section samples of the brain. RESULT : Though there was a difference of severity, rigidity was observed in both groups after MPTP administration. In the three cats in which the dorsal raphe nucleus was accurately included in the coagulated lesion, the tremor was observed after the seventh or eighth repetitive administration of MPTP. The tremor occurred in the resting position and lightly bending position of the limbs, and its cycle was similar to Parkinsonian tremor of 4〜6 Hz. In two cats, the tremor appeared throughout the 15〜40 minutes after MPTP administration, and gradually dissappeared. In one other cat, a similar tremor was observed after the eighth administration of MPTP, even on the clay when MPTP was not administered. In the cats of which the dorsal raphe nucleus was not destroyed accuratehr by coagulation, the tremor did not develop. CONCLUSION : Even in the cat, it was possible to create a resting tremor that is similar to Parkinsonian tremor. From the facts described above, we may conclude that damages both of the raphe nucleus serotonin system and the nucleus niger dopamine system may be involvecl in the mechanism for Parkinsonian tremor . Less

目的：帕金森氏病的主要病变是核尼日尔（Clopamineragric神经元）和Midbrain（去甲肾上腺素能神经元）的选择性神经元死亡。但是，我们不一定会仅通过这些损害部位来解释所有静止树的表现和机制。腹侧齿状（VMT）被称为包括猴子中帕金森 - 奥尼亚树的区域。我们报道说，尼日尔核和背部raphe核中的大量细胞被局部注射到猴子和猫中的VMT中。这项研究的目的是澄清损害尼日尔核的损害，而raphe核血清核神经元参与震颤的表现。方法：将15只成熟的猫分为两组：一组是背raphe核的凝结组，另一个是一个非凝结组。在凝血组中，尼日尔的损坏是在使用立体定向凝结技术破坏后raphe核的损害。在这两组中，MPTP（3 mg/kg/day）每隔一天每隔8-14次注射一次肌肉。然后在视频和肌电图中记录注射后的反应并分析。在大脑的串行截面样品中证实了凝结的病变。结果：尽管严重程度有差异，但在MPTP给药后两组都观察到了刚度。在凝结病变中准确包括背raphe核的三只猫中，在MPTP的第七次或第八次重复给药后观察到了树。这棵树发生在静止位置和四肢的轻微弯曲位置，其周期类似于4-6 Hz的帕金森氏树。在两只猫中，震颤在MPTP给药后的15-40分钟内出现，并逐渐消失。在另一只猫中，即使在未施用MPTP时，在第八次给药MPTP后也观察到类似的震颤。在那个猫中，背raphe核并未因凝结破坏精度，震颤没有发展。结论：即使在猫中，也可以创造出类似于帕金森震颤的休息树。从上面描述的事实来看，我们可能包括raphe核羟色胺系统和核尼日尔多巴胺系统的损害可能涉及帕金森氏震颤的机制。