Simplified measurements of the allergic released metallic ions within the oral cavit

口腔内过敏性释放金属离子的简化测量

• 批准号: 08672248
• 负责人: FURUKAWA Kazutoshi
• 金额: $ 1.28万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672248/
• 关键词: metal allergy; released metallic ion; simplified measurement; spectrophotometer; water analyzer; nickel ion; chromium ion; 口腔内イオン濃度; 簡易測定法; 機能的測定条件

The purpose of this study was to evaluate a new measuring method of released metallic ions, in order to clarify the mechanism of allergy. As compared with the conventional method by EPMA analysis of metal restorations, the advantages of this method are simplicity of handling and time saving. Firstly, it was determined the conditions nesessary for measurements of the released metallicions within the oral cavity. The basic experiment examined 3 types of measuring device, such as spectrophotometer, electrophotometer and visual colorimetric way. As a result, spectrophotometric measurement was selected. Secondly, the clinical experiments involed 6 subjects wearing occlusal mental splint on the maxillary dentition and the experimental splints were prepared Ni-Cr alloy and Co-Cr alloy which were considered to be dental materials having a high allergy rate. After lateral jaw movement during 5 minutes, it was attempted to judge on released metallic ions with the spectrophotometer and reagent set for water analyzer.THe results were as follows : 1) The average volumes of Cr-ion released from Ni-Cr alloy were 0.139mg/1 (maximum : 0.293mg/1, minimum : 0.06mg/1). 2) The average volumes of Ni-ion released from Ni-Cr alloy were 0.654mg/1 (maximum : 1.094mg/1, minimum : 0.496mg/1). 3) The average volumes of Cr-ion released from Co-Cr alloy were 0.08mg/1 (maximum : 0.141mg/1, minimum : 0.022mg/1).In conclusion, it can be said that the simplified measurements of released metallic ions were capable for clinical judgment on condition that were applied full mouth restrations.

本研究的目的是评估释放金属离子的新测量方法，以阐明过敏机制。与传统的金属修复体EPMA分析方法相比，该方法的优点是操作简单、节省时间。首先，确定了测量口腔内释放的金属离子所需的条件。基础实验考察了分光光度计、电子光度计和目视比色法3种测量装置。因此，选择分光光度测量。其次，对6名受试者进行了上颌牙列佩戴咬合金属夹板的临床实验，实验夹板制备的镍铬合金和钴铬合金被认为是过敏率较高的牙科材料。钳口横向移动5分钟后，尝试用分光光度计和水质分析仪试剂盒来判断释放的金属离子。结果如下： 1) Ni-Cr合金释放的Cr离子的平均量为0.139毫克/1（最大：0.293毫克/1，最小：0.06毫克/1）。 2) Ni-Cr合金释放的Ni离子平均量为0.654mg/1(最大：1.094mg/1，最小：0.496mg/1)。 3) Co-Cr合金释放的Cr离子的平均量为0.08mg/1（最大：0.141mg/1，最小：0.022mg/1）。综上所述，可以说，释放金属的简化测量离子能够对全口复位的情况进行临床判断。