Microcircutation in burned wound and usefulness of microcirculation modulation

烧伤创面的微循环和微循环调节的作用

• 批准号: 08671383
• 负责人: SHINOZAWA Yotaro
• 金额: $ 1.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671383/
• 关键词: burn shock; microcirculation; nitric oxide synthase inhibitor; nitric oxide; inducible nitric oxide synthase; constitutive nitric oxide synthase; endotoxin; nafamostat mesilate; エンドトキセミア; 蛋白分解酵素阻害薬; 一酸化窒素合成酵素阻害薬; 熱傷; 熱傷創; 創傷治癒; カテコラミン; ハイドロ; burn shock; microcirculation; nitric oxide synthase inhibitor; nitric oxide; inducible nitric oxide synthase; constitutive nitric oxide synthase; endotoxin; nafamostat mesilate; エンドトキセミア; 蛋白分解酵素阻害薬; 一酸化窒素合成酵素阻害薬; 熱傷; 熱傷創; 創傷治癒; カテコラミン; ハイドロ

1. The effects of microcirculation in a third degree burned wound in the early phase of burn on the integrity was studied by evaluating insensible water loss (ISWL) in 30% TBSA burned SD rat. The effects of cNOS inhibitor (N^G-nitro-L-arginine 10 mg/kg) and iNOS (aminoguanidine 100 mg/kg) on urinary NOx (=N02+N03) excretion and on ISWL were also examined. Urinary NOx was considered to be excreted dominantly by iNOS and iNOS inhibitor seemed to be beneficial to maintain the microcirculation in burned wound.2. To search a clinically available iNOS inhibitor, sepsis rat model induced by repeatedly (five times) injected endotoxin (5-10 mg/kg) for 100 minutes was used. FUT-175 (clinically used as a protease iinhibitor) was examined as to protect iNOS dependent organ (liver, lung and stomach) injuries. Endotoxemia increased plasma NOx levels from 19.4+/-2.1 micro MIL of control levels up to 36.0+/-4.1, and decreased down to 26.5+1-7.4 by FUT-175 (1-2 mg/kg x2) administration. cNOS in liver and lung increased in endotoxemia, and iNOS increased in all examined organs by injection of endotoxin. FUT-175 administration decreased the increased iNOS activities to control levels.3. iNOS seemed to be dominant both in the early phase and in septic phase of burn, and NO modulation by FUT-175 is considered useful for maintain circulation and protection organ function in burned patients.

1。通过评估30％TBSA燃烧的SD大鼠的不可知的水损失（ISWL），研究了第三度烧伤伤口对完整性的微循环的影响。 CNOS抑制剂（N^G-Nitro-L-精氨酸10 mg/kg）和iNOS（氨基瓜氨酸100 mg/kg）对尿液NOX（= N02+N03）排泄的影响以及在ISWL上的排泄。尿液被认为被iNOS显着排泄，而iNOS抑制剂似乎对维持燃烧伤口的微循环是有益的。2。为了搜索临床上可用的INOS抑制剂，使用反复（5次）注射内毒素（5-10 mg/kg）诱导的败血症大鼠模型100分钟。检查了FUT-175（临床用作蛋白酶i抑制剂），以保护依赖iNOS的器官（肝脏，肺和胃）伤害。内毒素血浆NOX水平从19.4 +/- 2.1微米的控制水平提高到36.0 +/- 4.1，而FUT-175（1-2 mg/kg x2）给药降低至26.5+1-7.4。肝脏中的CNO和肺内毒素血症中的CNO升高，并且通过注射内毒素的所有检查器官中的iNOS均增加。 FUT-175政府减少了iNOS活动增加以控制水平。3。 INOS在早期和燃烧的化粪池阶段似乎都是占主导地位的，而FUT-175的调节无被认为可用于维持烧伤患者的循环和保护器官功能。