Chromosomal Translocations in Human Soft Tissue Sarcomas : Fluorescence in Situ Hydridization as a Diagnostic Aid

人类软组织肉瘤中的染色体易位：荧光原位杂交作为诊断辅助手段

• 批准号: 08670203
• 负责人: YOSHIDA Haruhiko
• 金额: $ 1.41万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670203/
• 关键词: soft tissue sarcoma; paraffin-block; chromosome; translocation; fluorescence in situ hybridization (FISH); 染色体異常; マルチカラーFISH法

To evaluate fluorescence in stu hybridization (FISH) findings of soft tissue sarcomas and clarify the relation between their hisological tumor types and chromosomal rearrangements, we examined three kinds of translocations in the interphase nuclei isolated from paraffin-embedded tissues of 47 soft tissue sarcomas. Translocations were evaluated using chromosome-specific DNA probes of centromeric and whole painting probes.Of 47 soft tissue sacomas, 42 (89.4%) revealed timor-specific translocations by etrospective chromosomal analysis. Translocation t(X ; 1 8) was detected in 25/28 synovi al sarcomas ; translocation t(11 ; 22) in 5/6 Ewing's sarcomas and primitive neuroectodermal tumors (PNET) ; and translocation t(12 ; 16) was found in 12/13 liposarcomas (LPS), including 10 myxoid and 2 round cell types as don aichrom osoma I aberrations specific for both subtypes.Based on these findings, Ewing's sarcoma is related closely with PNET originating in neuroectodermal tissue. Other cytogenetic finding of translocation t(12 ; 16) indicates the round cell liposarcoma shares chromosomal change with myxoid LPS, and further suggests that both tumors may possess common prcursor cells.Karyotypic analysis was performed on a case of chondromyxoid fibroma arising in 42-year-old, female and revealed a complex pattern of chromosomal abberation including t(1 ; 12).

为了评估软组织肉瘤的Stu杂交（FISH）发现的荧光，并阐明其神性肿瘤类型与染色体重排之间的关系，我们检查了从47个软组织肉瘤的冰蛋白包含的组织中分离出的相对核中的三种易位核。使用染色体特异性的DNA探针评估了丝粒和整个涂料探针的DNA探针。47个软组织sacomas，42（89.4％）揭示了通过Etrosportive染色体分析表明Timor特异性的易位。在25/28的Synovi al肉瘤中检测到易位t（x; 1 8）； 5/6 ewing的肉瘤和原始神经外科肿瘤（PNET）中的易位t（11; 22）；在12/13脂肪肉瘤（LPS）中发现了易位t（12; 16），其中包括10种粒细胞类型和2种圆形细胞类型，作为对两个亚型的特有的don Aichrom osoma I的畸变。基于这些发现，Ewing的肉瘤与源自神经外科医生的PNET与PNET相关。易位T（12; 16）的其他细胞遗传学发现表明，圆形细胞脂肪肉瘤与粒细胞类动物LPS共享染色体变化，进一步表明，这两个肿瘤可能具有共同的prcursortor细胞。Karyotypic分析是在42岁的女性，女性，呈现铬s骨均具有复杂模式的charyomxoid纤维瘤上进行的。

项目成果

Yoshida H,Nagao K,Endo H,Ito H: "Search for Chromosomal Translocation by Fluorescence in Situ Hybridization Method (in Japanese)" Pathol Clin Med. 16 (2). 156-160 (1998)

Yoshida H、Nagao K、Endo H、Ito H：“通过荧光原位杂交方法寻找染色体易位（日语）”Pathol Clin Med。

Osaki M, Shimabayashi A, Iwao Y, Asakura Y, Adachi H, Ito H and Yoshida H reactions.: "Search for mycobacterial DNA in paraffin-embedded tissues from bone and soft-tissue tuberculosis by duplex polymerase chain Ahelpful methodto histopathological diagnosi

Osaki M、Shimabayashi A、Iwao Y、Asakura Y、Adachi H、Ito H 和 Yoshida H 反应。：“通过双链聚合酶链在骨和软组织结核病的石蜡包埋组织中搜索分枝杆菌 DNA 组织病理学诊断的有用方法

Osaki M, Adachi H, Gomyo Y, Yoshida H and Ito H: "Detection of Mycobacterial DNA in Formalin-Fixed,Paraffin-Embedded Tissue Specimensa by Duplex Polymerase Chain Reaction : Application to Histopathologic Diagnosis" Mod Pathol. 78. 78-83 (1997)

Osaki M、Adachi H、Gomyo Y、Yoshida H 和 Ito H：“通过双链聚合酶链反应检测福尔马林固定、石蜡包埋的组织标本中的分枝杆菌 DNA：在组织病理学诊断中的应用”Mod Pathol。

Osaki M, Adachi H, Gomyo Y, Yoshida H and Ito H: "Detection of mycobacterial DNA in formalin-fixed, paraffin-embeddedtissue specimens by duplex polymerase chain reaction : Application to histopathologic diagnosis" Mod Pathol. 10. 78-83 (1997)

Osaki M、Adachi H、Gomyo Y、Yoshida H 和 Ito H：“通过双重聚合酶链反应检测福尔马林固定、石蜡包埋的组织标本中的分枝杆菌 DNA：在组织病理学诊断中的应用”Mod Pathol。

Yoshida H,Nagao K,Ito H,Yamamoto K and Ushigome S: "Chromosomal translocations in human soft tissue sarcomas : Fluorescence in situ hybridization as a diagnostic aid" Pathol Int. 47. 222-229 (1997)

Yoshida H、Nagao K、Ito H、Yamamoto K 和 Ushigome S：“人类软组织肉瘤中的染色体易位：荧光原位杂交作为诊断辅助” Pathol Int。