Evaluation for functional correlation between prostaglandins and dopamine in regulation mechanisms of ADH release in the AV3V

AV3V 中 ADH 释放调节机制中前列腺素和多巴胺之间功能相关性的评估

基本信息

批准号：
08670076
负责人：
YAMAGUCHI Kenichi
金额：
$ 1.22万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

1. The aims of reseach project were to examine roles of prostaglandins (PGs) synthesized in the anteroventral third ventricular region (AV3V) in regulation mechanisms of ADH release and cardiovascular system under hypovolemic condition, and to evaluate those of AV3V catecholamine receptors in mediating responses to PGE2. Experiments were conducted in conscious rats with cerebral and vascular cannulae implanted chronically.2. Blood of 1% of body weight was removed twice (H1 and H2) from the femoral artery. H1 increased plasma ADH (pADH) without affecting arterial pressure (AP) and heart rate (HR), whereas after H2 further augmentation of pADH were produced with conspicuous falls in AP and rises in plasma angiotensin II (ANGII). These homorrhages did not alter plasma osmolality or sodium, but increased plasma chloride and decreased plasma potassium.3. An inhibitor of PG synthesis meclofenamate (MCL) infused locally into the AV3V prevented the pADH responses to the bleeding, potentiated t … More hose of AP, and caused singnificant reduction of HR following H2. Other variable such as plasma ANGII were not influenced by MCL.These effects of MCL were not caused when it was infused into the nucleus accumbens, a region sligtlhy distant from the AV3V,or into the cerebral ventricle.4. When PGE2 was applied into the AV3V sites near infusion points of MCL,pADH,AP and HR increased singnificantly.5. Local infusion of dopamine (DA) and an alpha-adrenegic agonist phenylephrines (PHE) into the AV3V elicited transient augmentation of pADH.DA influenced none of the other variables, whereas PHE application caused concomitant reises of AP in approximately 50% of tests. The AV3V infusion of a beta-adrenergic agonist isoproterenol induced depressor and tachycardiac effects.6. The effects of PHE on both the pADH and AP were abolished by prior application of an alpha-adrenergic antagonist phenoxybenzamine into the AV3V.The effect of DA on pADH was blocked by the prior administration of a D1-DAergic antagonist SCH23390, but not by that of a D2-DAergic antagonist sulpiride. The application of these antagonists alone did not affect any of the variables including pADH.7. The AV3V infusion of phenoxybenzamine, SCH23390 and sulpiride were without significant effect on the PGE2-evoed responses of pADH,AP and HR.8. These results suggest that PGs formed in the AV3V may play important roles in regulating ADH release, AP and HR,and that the effects of PGE2 on these factors may not be mediated by AV3V catecholamine receptors, despite their ability to influence the factors. Less

1。参考项目的目的是检查前腹部第三心室区域（AV3V）在ADH释放的调节机制中合成的前列腺素（PGS）的作用，在低血容量状态下的ADH释放机制和心血管系统的调节机制，并评估AV3V可介胺受体在介导的反应中对PGE2的介导的响应反应中的aDH释放和心血管系统的作用。在有意识的大鼠中，长期植入了脑和血管插管2。从股骨伪影中除去了两次体重的血液两次（H1和H2）。 H1增加了血浆ADH（PADH）而不会影响动脉压（AP）和心率（HR），而H2后，PADH的进一步增强在AP中显着下降并在血浆血管紧张素II（Angii）中升高。这些同句不会改变血浆渗透压或钠，而是增加了血浆氯化物并减少血浆钾3。局部感染AV3V的PG合成麦氯氯芬酰胺（MCL）的抑制剂阻止了PADH对出血的反应，潜在的T…更多的AP软管，并在H2后引起HR的单人降低。其他变量（例如等离子体ANGII）不受MCL的影响。当将其感染到伏隔核，远离AV3V的区域或大脑通气中时，MCL的这些作用并不是引起的。4。当将PGE2应用于MCL的输液点附近的AV3V位点时，padh，ap和HR偶然增加了。5。局部输注多巴胺（DA）和α-亚辅导激动剂苯肾上腺素（PHE）中的AV3V引起的PADH.DA的瞬态增强均未影响其他变量，而PHE应用在大约50％的测试中引起了PHE的应用。 AV3V输注β-辅亚激动剂异丙肾上腺素诱导的抑制剂和心动过速效应。6。 PHE对PADH和AP的影响通过事先应用于AV3V中的α-肾上腺素拮抗剂苯氧苯甲胺。仅这些拮抗剂的应用不会影响任何变量，包括PADH.7。苯氧苯甲胺，SCH23390和硫磺的AV3V输注对PADH，AP和HR.8的PGE2验反应没有显着影响。这些结果表明，在AV3V中形成的PG可能在调节ADH释放AP和HR中起重要作用，并且PGE2对这些因素的影响可能不会由AV3V Catecholamine受体介导，从而影响了这些因素。较少的