Analysis of the intracellular signal pathway in the pancreatic b-cells by direct observation of exocytosis

通过直接观察胞吐作用分析胰腺 b 细胞的细胞内信号通路

• 批准号: 08670046
• 负责人: TERAKAWA Susumu
• 金额: $ 1.41万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670046/
• 关键词: beta-cell; exocytosis; insulin; super oxide; chemiluminescence; video microscopy; vitamin E; MCLA; ラジカル

The purpose of the present study is to clarify the involvement of major radical species on the insulin release from the pancreatic beta-cells. To this end, we applied NO,O_<2">, and alpha-tochopherol on the islet cells. These radicals induced degranulation of the beta-cells. We also observed chemiluminescene in the pancreatic tissue in vivo perfused with MCLA containing medium. Stimulation of the tissue with glucose significantly increased the MCLA-dependent chemiluminescence especially in the vicinity of the islet. Therefore, it was concluded that elevation in the glucose concentration results in radical production in the tissue and radicals induces degranulation in the beta-cells. alpha-tocopherol also induced degranulation in the same tissue. This effect of alpha-tocopherol was blocked by some types of bovine serum albumin. The present study clarified that radicals have not only adverse effects on the beta-cell but also physiologically important role in signal pathway leading to insulin release. It should be also emphasized that the beta-cells has a special sensitivity to radicals, and some radical scavengers act as radical species in the b-cells,

本研究的目的是阐明主要自由基种类对胰腺β细胞释放胰岛素的影响。为此，我们在胰岛细胞上应用NO、O_<2">和α-生育酚。这些自由基诱导β细胞脱粒。我们还在体内灌注含MCLA培养基的胰腺组织中观察到化学发光。用葡萄糖处理组织显着增加了 MCLA 依赖性化学发光，特别是在胰岛附近。因此，得出结论，葡萄糖浓度的升高导致组织中自由基的产生。自由基会诱导 α-生育酚在同一组织中发生脱粒，而某些类型的牛血清白蛋白会阻断α-生育酚的这种作用。本研究阐明，自由基不仅对 β 细胞有不利影响。 β细胞在导致胰岛素释放的信号通路中也发挥着重要的生理作用。还应该强调的是，β细胞对自由基具有特殊的敏感性，并且一些自由基清除剂充当自由基种类。 B细胞，

项目成果

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Terakawa, S.: "Dynamics of exocytotic release of catecholamine measured simultancously by VEC-DIC microscopy and DC-amperometry" Jpn.J.Physiol.46. S7- (1996)

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T.Sakurai and S.Terakawa: "Glucose-induced superoxide production in pancreatic tissues and superoxide-induced degranulation." Photomedicine and Photobiology. 18. S9 (1997)

T.Sakurai 和 S.Terakawa：“胰腺组织中葡萄糖诱导的超氧化物产生和超氧化物诱导的脱颗粒。”

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