Studies on the genome heterogeneity and its host passage effect of apple stem grooving capillovirus

苹果茎沟毛细病毒基因组异质性及其宿主传代效应研究

• 批准号: 08660047
• 负责人: YOSHIKAWA Nobuyuki
• 金额: $ 1.41万
• 依托单位: IWATE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660047/
• 关键词: Capillovirus; apple stem grooving virus; sequence variants; genome hetetogenity; host passage effect; SSCP; ASGV

The 3'-terminal regions of the genomes of apple stem grooving virus (ASGV) isolated from apple, Japanese pear and European pear plants and citrus tatter leaf virus (CTLV) from citrus plants were amplified by the reverse transcription-polymerase chain reaction and then the DNA products were sequenced. The results indicated that all ASGV isolates from apple, Japanese pear and European pear comprise at least two to four 'sequence variants' which significantly differ in nucleotide sequence from each other. Comparison of the amino acid (aa) sequences of the ORF1 and ORF2 proteins among isolates or sequence variants including isolates of ASGV and CTLV reported previously showed that the aa sequences of the ORF2 protein (320 aa) and the putative coat protein (CP) (237aa) equivalent to aa positions 1868 to 2105 of the ORF1 protein of ASGV (P-209) were highly conserved among isolates or sequence variants.Similarities ranged from 92.8 to 100% for the ORF2 protein among 15 isolate or sequence variants and 93.2 to 100% for the CP among 21 isolates or sequence variants. On the contrary, the aa sequence similarities of a284 aa region (named 'V-region' in this paper) equivalent to aa positions 1584 to 1868 of the ORF1 protein which encodes ORF2 protein in another frame were highly variable (53.5 to 99.3%) among 15 isolates and sequence variants. The percentage of conserved V-region aa in all 15 was only 20.4%. Phylogenetic trees constructed from the aa sequences of CP,ORF2 or V-region proteins showed that isolates or sequence variants were separated into several clusters, regardless of the source host plant species (apple, Japanese pear, European pear, citrus and lily). In a tree constructed by V-region proteins, two CTLV isolates were grouped into two different clusters each of which contained isolates or sequence variants from apple and Japanese pear. These results demonstrate that CTLV from citrus cannot be distinguished from ASGV from Rosaceous plants at the molecular level.

通过逆转录聚合酶链式反应扩增从苹果、日本梨和欧洲梨植物中分离的苹果茎沟病毒（ASGV）和从柑橘植物中分离的柑橘碎叶病毒（CTLV）的基因组3'端区域，然后DNA产物被测序。结果表明，来自苹果、日本梨和欧洲梨的所有ASGV分离株都包含至少两到四个“序列变体”，这些变体在核苷酸序列上彼此显着不同。之前报道的分离株或序列变体（包括 ASGV 和 CTLV 分离株）之间 ORF1 和 ORF2 蛋白的氨基酸（aa）序列的比较表明，ORF2 蛋白（320 个氨基酸）和推定外壳蛋白（CP）的氨基酸序列（ 237aa) 相当于 ASGV (P-209) ORF1 蛋白 1868 至 2105 位氨基酸，在分离株或序列中高度保守15 个分离株或序列变体之间的 ORF2 蛋白相似性范围为 92.8% 至 100%，21 个分离株或序列变体之间的 CP 相似性范围为 93.2% 至 100%。相反，相当于另一框架中编码ORF2蛋白的ORF1蛋白的氨基酸位置1584至1868的a284氨基酸区（本文中称为“V区”）的氨基酸序列相似性高度可变（53.5%至99.3%） 15 个分离株和序列变体。 15个氨基酸中保守的V区氨基酸比例仅为20.4%。由 CP、ORF2 或 V 区蛋白的 aa 序列构建的系统发育树表明，无论来源宿主植物种类如何（苹果、日本梨、欧洲梨、柑橘和百合），分离株或序列变体都分为几个簇。在由 V 区蛋白构建的树中，两种 CTLV 分离株被分为两个不同的簇，每个簇都包含来自苹果和日本梨的分离株或序列变体。这些结果表明，来自柑橘的 CTLV 无法在分子水平上与来自蔷薇科植物的 ASGV 区分开。

项目成果

Ysihikawa, N., Sasamoto, K., Sakurada, M., Takahashi, T.and Yanase, H.: "Apple stem grooving and cotrus tatter leaf capilloviruses obtained from a single shoot of Japanese pear (Pyrus serotina)." Annals of the Phytopathological Society of Japan. vol.62, N

Ysihikawa, N.、Sasamoto, K.、Sakurada, M.、Takahashi, T. 和 Yanase, H.：“从日本梨 (Pyrus serotina) 的单芽中获得苹果茎沟和柑橘碎叶毛状病毒。”

Magome, H., Yoshikawa et al.: "Molecular Variability of the genome of capilloviruses from apple,Japanese pear,European pear, and citrus trees" Phytopathology. 87・4. 389-396 (1997)

Magome, H., Yoshikawa 等：“来自苹果、日本梨、欧洲梨和柑橘树的毛状病毒基因组的分子变异”植物病理学 87・4 (1997)。

Magome, H.Yoshikawa, N., Takahashi, T., Ito, T., Miyakawa, T.: "Molecular variability of the genomes of capilloviruses from apple, Japanese Pear, European pear, and citrus trees." Phytopathology. vol.87, N0.4. 389-396 (1997)

Magome, H.Yoshikawa, N.、Takahashi, T.、Ito, T.、Miyakawa, T.：“来自苹果、日本梨、欧洲梨和柑橘树的毛状病毒基因组的分子变异性。”

Yoshikawa, N.et.al.: "Apple stem grooving and citrus tatter leaf capillovirus obtained from a single shoot of Japanese pear (Pyrus serotina)" Annals of the Phytopathological Society of japan. 62・2. 103-108 (1996)

Yoshikawa，N.et.al.：“从日本梨（Pyrus serotina）单枝中获得的苹果茎沟和柑橘碎叶毛状病毒”，日本植物病理学会年鉴 62·2（1996）。

H.Magome,N.Yoshikawa,et al.: "Molecular ariability of the genome of capilloviruses from apple,Japanese pear,European pear and citrus frees" Phytopathology. 87(印刷中). (1997)

H. Magome、N. Yoshikawa 等人：“来自苹果、日本梨、欧洲梨和柑橘的毛细病毒基因组的分子变异性” 植物病理学 87（出版中）。