Studies on degradation mechanism of petroleum under anoxic condition.

缺氧条件下石油降解机理研究。

• 批准号: 08650942
• 负责人: MORIKAWA Masaaki
• 金额: $ 1.47万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08650942/
• 关键词: anoxic condition; petroleum degrading bacteria; alkane; alkene; HD-1; TK-122; Molibdenate; electron acceptor; 嫌気条件; 不飽和結合; ノードデセン; 飽和炭化水素; ^1H-NMR; GC-MS; n-テトラデカン; anoxic condition; petroleum degrading bacteria; alkane; alkene; HD-1; TK-122; Molibdenate; electron acceptor; 嫌気条件; 不飽和結合; ノードデセン; 飽和炭化水素; ^1H-NMR; GC-MS; n-テトラデカン

Bacterial strain HD-1, isolated from an oil field, is able to degrade both aliphatic and aromatic hydrocarbons anaerobically. In this project, bacterial degradation mechanisms of petroleum under anoxic condition was studied Utilizing tetradecane (alkane) as a substrate, two kinds of alkene were accumulated as degradation intermediates in HD-1 cells. Finally a main component of the alkenes was identified as 1-dodecene based on analyzes data from FT-IR,1H-NMR,and GC/MS.This result shows that alkane is dehydrogenated by the strain HD-1 under anoxic condition. Although one example was reported in 1992 from a research group in Germany about alkane degradation under anoxic condition, this is the first report on the structural analysis of a degradation intermediate. Moreover, strain HD-1 were found to be able to grow on CO2 as a sole carbon source and produce alkane in the cells. Its production yield was 0.1% in dry cell weight. The metabolic pathway from CO2 to alkane needs to be elucidated in a future.Recently, a new bacterial strain TK-122 was isolated oil-reservoir tank. Its oil degradation ability is superior to HD-1 under both oxic and anoxic conditions. About 70% of alkanes (from octane to hexadecane) in petroleum, was degraded in 3 days under anoxic condition. Molibdenate (MoO4^<2->) was used as an electron acceptor in the anaerobic degradation of alkane. This result indicates a finding of a new petroleum degradation pathway. According to the analysis on 16S rRNA nucleotide sequence of the strain TK-122, Escherichia or Citrobacter was most probable genus. However, physiological characterization of the strain matched with none of these genera. It is indicated that the strain TK-122 belongs to a new genus.

从油田中分离出的细菌菌株HD-1能够厌氧地降解脂肪族和芳烃。在该项目中，利用四烷（Alkane）作为底物研究了石油在缺氧条件下的细菌降解机制，在HD-1细胞中积累了两种烯烃作为降解中间体。最终，基于FT-IR，1H-NMR和GC/MSS的分析，烯烃的主要成分被鉴定为1二十烯，这表明烷烃在缺氧条件下被HD-1菌株脱氢。尽管1992年，德国的一个研究小组报告了一个例子，涉及在缺氧条件下烷烃降解，但这是关于降解中间体结构分析的第一个报告。此外，发现HD-1菌株能够在CO2上生长为唯一的碳源并在细胞中产生烷烃。其生产产量为干细胞重量为0.1％。从二氧化碳到烷烃的代谢途径需要在将来阐明。绝对，新的细菌菌株TK-122被分离为油砂罐。在氧和缺氧条件下，其油脂降解能力优于HD-1。在石油上约有70％的烷烃（从辛烷到十六进制）在缺氧条件下3天降解。在烷烃的厌氧降解中，将molibdenate（MOO4^<2->）用作电子受体。该结果表明发现了新的石油降解途径。根据菌株TK-122菌株的16S rRNA核苷酸序列的分析，大肠杆菌或柠檬酸菌是最可能的属。然而，与这些属匹配的菌株的生理表征。据表明，TK-122菌株属于新属。