Studies on degradation mechanism of petroleum under anoxic condition.

缺氧条件下石油降解机理研究。

基本信息

批准号：
08650942
负责人：
MORIKAWA Masaaki
金额：
$ 1.47万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08650942/
关键词：
anoxic condition petroleum degrading bacteria alkane alkene HD-1 TK-122 Molibdenate electron acceptor 嫌気条件不飽和結合ノードデセン飽和炭化水素 ^1H-NMR GC-MS n-テトラデカン

项目摘要

Bacterial strain HD-1, isolated from an oil field, is able to degrade both aliphatic and aromatic hydrocarbons anaerobically. In this project, bacterial degradation mechanisms of petroleum under anoxic condition was studied Utilizing tetradecane (alkane) as a substrate, two kinds of alkene were accumulated as degradation intermediates in HD-1 cells. Finally a main component of the alkenes was identified as 1-dodecene based on analyzes data from FT-IR,1H-NMR,and GC/MS.This result shows that alkane is dehydrogenated by the strain HD-1 under anoxic condition. Although one example was reported in 1992 from a research group in Germany about alkane degradation under anoxic condition, this is the first report on the structural analysis of a degradation intermediate. Moreover, strain HD-1 were found to be able to grow on CO2 as a sole carbon source and produce alkane in the cells. Its production yield was 0.1% in dry cell weight. The metabolic pathway from CO2 to alkane needs to be elucidated in a future.Recently, a new bacterial strain TK-122 was isolated oil-reservoir tank. Its oil degradation ability is superior to HD-1 under both oxic and anoxic conditions. About 70% of alkanes (from octane to hexadecane) in petroleum, was degraded in 3 days under anoxic condition. Molibdenate (MoO4^<2->) was used as an electron acceptor in the anaerobic degradation of alkane. This result indicates a finding of a new petroleum degradation pathway. According to the analysis on 16S rRNA nucleotide sequence of the strain TK-122, Escherichia or Citrobacter was most probable genus. However, physiological characterization of the strain matched with none of these genera. It is indicated that the strain TK-122 belongs to a new genus.

从油田分离出来的细菌菌株 HD-1 能够厌氧降解脂肪族和芳香族碳氢化合物。本项目研究缺氧条件下细菌降解石油的机理，以十四烷（烷烃）为底物，在HD-1细胞中积累两种烯烃作为降解中间体。最后根据FT-IR、1H-NMR和GC/MS的分析数据，鉴定出烯烃的主要成分为1-十二碳烯。该结果表明菌株HD-1在缺氧条件下将烷烃脱氢。尽管德国的一个研究小组在1992年报道了缺氧条件下烷烃降解的例子，但这是第一篇关于降解中间体结构分析的报告。此外，菌株HD-1被发现能够以二氧化碳作为唯一碳源生长并在细胞中产生烷烃。其产率为细胞干重的0.1%。从CO2到烷烃的代谢途径有待进一步阐明。最近，在储油罐中分离出一株新菌株TK-122。在有氧和缺氧条件下其油降解能力均优于HD-1。石油中约70%的烷烃（从辛烷到十六烷）在缺氧条件下3天内被降解。钼酸盐(MoO4^2-)被用作烷烃厌氧降解中的电子受体。该结果表明发现了新的石油降解途径。根据菌株TK-122的16S rRNA核苷酸序列分析，最可能的属是埃希氏菌属或柠檬酸杆菌属。然而，该菌株的生理特征与这些属都不匹配。这表明菌株TK-122属于一个新属。