Studies on degradation mechanism of petroleum under anoxic condition.
缺氧条件下石油降解机理研究。
基本信息
- 批准号:08650942
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Bacterial strain HD-1, isolated from an oil field, is able to degrade both aliphatic and aromatic hydrocarbons anaerobically. In this project, bacterial degradation mechanisms of petroleum under anoxic condition was studied Utilizing tetradecane (alkane) as a substrate, two kinds of alkene were accumulated as degradation intermediates in HD-1 cells. Finally a main component of the alkenes was identified as 1-dodecene based on analyzes data from FT-IR,1H-NMR,and GC/MS.This result shows that alkane is dehydrogenated by the strain HD-1 under anoxic condition. Although one example was reported in 1992 from a research group in Germany about alkane degradation under anoxic condition, this is the first report on the structural analysis of a degradation intermediate. Moreover, strain HD-1 were found to be able to grow on CO2 as a sole carbon source and produce alkane in the cells. Its production yield was 0.1% in dry cell weight. The metabolic pathway from CO2 to alkane needs to be elucidated in a future.Recently, a new bacterial strain TK-122 was isolated oil-reservoir tank. Its oil degradation ability is superior to HD-1 under both oxic and anoxic conditions. About 70% of alkanes (from octane to hexadecane) in petroleum, was degraded in 3 days under anoxic condition. Molibdenate (MoO4^<2->) was used as an electron acceptor in the anaerobic degradation of alkane. This result indicates a finding of a new petroleum degradation pathway. According to the analysis on 16S rRNA nucleotide sequence of the strain TK-122, Escherichia or Citrobacter was most probable genus. However, physiological characterization of the strain matched with none of these genera. It is indicated that the strain TK-122 belongs to a new genus.
从油田中分离出的细菌菌株HD-1能够厌氧地降解脂肪族和芳烃。在该项目中,利用四烷(Alkane)作为底物研究了石油在缺氧条件下的细菌降解机制,在HD-1细胞中积累了两种烯烃作为降解中间体。最终,基于FT-IR,1H-NMR和GC/MSS的分析,烯烃的主要成分被鉴定为1二十烯,这表明烷烃在缺氧条件下被HD-1菌株脱氢。尽管1992年,德国的一个研究小组报告了一个例子,涉及在缺氧条件下烷烃降解,但这是关于降解中间体结构分析的第一个报告。此外,发现HD-1菌株能够在CO2上生长为唯一的碳源并在细胞中产生烷烃。其生产产量为干细胞重量为0.1%。从二氧化碳到烷烃的代谢途径需要在将来阐明。绝对,新的细菌菌株TK-122被分离为油砂罐。在氧和缺氧条件下,其油脂降解能力优于HD-1。在石油上约有70%的烷烃(从辛烷到十六进制)在缺氧条件下3天降解。在烷烃的厌氧降解中,将molibdenate(MOO4^<2->)用作电子受体。该结果表明发现了新的石油降解途径。根据菌株TK-122菌株的16S rRNA核苷酸序列的分析,大肠杆菌或柠檬酸菌是最可能的属。然而,与这些属匹配的菌株的生理表征。据表明,TK-122菌株属于新属。
项目成果
期刊论文数量(14)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
森川 正章 ら: "炭酸ガスからの石油生成" 農芸化学会誌. 72・4. 532-534 (1998)
Masaaki Morikawa 等:“从二氧化碳生产石油”日本农业化学学会杂志 72・4(1998 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Imanaka,M.Morikawa: "A New Mixotrophic Bacterium That Can Fix Co_2 and Assimilate Aliphatic and Aromatic Hydrccarbons Anaerobically" In Molecular Biology of Pseudomonads. Chapter25. 289-297 (1996)
T.Imanaka,M.Morikawa:“一种可以固定 Co_2 并厌氧同化脂肪族和芳香族烃的新型混合营养细菌”,《假单胞菌分子生物学》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Morikawa, T.Iwasa, S.Yanagida and T.Imanaka.: "Production of alkane and alkene from CO2 by a petroleum degrading bacterium." J.Ferment.Bioeng.85. 243-245 (1998)
M.Morikawa、T.Iwasa、S.Yanagida 和 T.Imanaka.:“通过石油降解细菌从 CO2 生产烷烃和烯烃。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Morikawa,M.Kanemoto,T.Imanaka: "Biological Oxidation of Alkane to Alkene under Anaerobic Conditions" Journal of Fermentation and Bioengineering. 82・3. 309-311 (1996)
M.Morikawa、M.Kanemoto、T.Imanaka:“厌氧条件下烷烃生物氧化为烯烃”《发酵与生物工程杂志》82・3(1996)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Imanaka and M.MORIKAWA: "A new mixotrophic bacterium that can fix CO_2 and assimilates aliphatic and arohratic hydrocarbons anaerobically" Molecular Biology to Pseudomonads. 289-297 (1996)
T.Imanaka 和 M.MORIKAWA:“一种新型混合营养细菌,可以固定 CO_2 并以厌氧方式同化脂肪族和芳香族碳氢化合物”假单胞菌的分子生物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MORIKAWA Masaaki其他文献
MORIKAWA Masaaki的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MORIKAWA Masaaki', 18)}}的其他基金
Discovery and understanding of novel aquatic plants-microbial interactions
新型水生植物-微生物相互作用的发现和理解
- 批准号:
16K14844 - 财政年份:2016
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Studies on the primitive alkane metabolisms and ecology of extremely thermophilic petroleum bacteria
极高温石油菌原始烷烃代谢及生态学研究
- 批准号:
22380175 - 财政年份:2010
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analyses on the molecular mechanisms of biofilm formation that reveal unknown functions of microorganisms
分析生物膜形成的分子机制,揭示微生物的未知功能
- 批准号:
19380189 - 财政年份:2007
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
相似国自然基金
Rpf调控VBNC细菌复苏介导土壤石油污染物高效降解的微生态效应
- 批准号:42267017
- 批准年份:2022
- 资助金额:34 万元
- 项目类别:地区科学基金项目
食细菌线虫调控细菌群落影响污染土壤石油降解的机理研究
- 批准号:31460149
- 批准年份:2014
- 资助金额:50.0 万元
- 项目类别:地区科学基金项目
海洋石油细菌T7-7降解烷烃的分子机制的研究和功能优化
- 批准号:31270133
- 批准年份:2012
- 资助金额:80.0 万元
- 项目类别:面上项目
相似海外基金
Degradative matrix construction with aromatic condensed heterocyclic compounds by petroleum desulfurizing and PAH degrading bacteria, and its application for bioremediation.
石油脱硫多环芳烃降解菌构建芳香稠合杂环化合物降解基质及其生物修复应用
- 批准号:
17K08173 - 财政年份:2017
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Detection and quantification of petroleum hydrocarbon degrading bacteria targeting catechol cleavage genes
针对儿茶酚裂解基因的石油烃降解菌的检测和定量
- 批准号:
10680544 - 财政年份:1998
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)